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V A Agranenko

Publications and source records attributed to V A Agranenko.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Effect of pH on the regulatory characteristics of energy metabolism in human erythrocytes].

The glucose consumption rate versus ATP content in human red cells (regulatory patterns of glycolysis) and ATP concentration versus glucose uptake rate in red cell suspension (regulatory patterns of total ATPases), when the rate of glucose uptake is constant and lower than the rate of glucose consumption at physiological conditions, were measured at different pH values. The shape of both types of kinetic curves was found to be dependent on the pH of the incubation medium but the same for the red cells taken from different donors. It is supposed that at alkaline pH, glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase reactions become the rate-limiting steps of glycolysis instead of hexokinase and phosphofructokinase under physiological conditions.

Adenosine Triphosphate↗
PubMed1985

ADP-induced refractoriness of platelets preserved in different temperature conditions.

Storage of platelets is known to cause significant cell alterations dependent on temperature. The ability of developing the refractory state in platelets exposed to different temperature conditions of preservation was investigated. PC storage at 4 degrees C was shown to produce a decrease of platelet ability to transform to the refractory state under action of ADP and a storage at 22 degrees C resulted in an increase of this ability. The inverse correlation between refractoriness and aggregation was found in platelets preserved at 22 degrees C and in combined temperature conditions. The effects observed were supposed to be related to changes in the platelet adenylate cyclase system.

Adenosine Diphosphate↗
PubMed1984

Transformations of glycolysis control characteristics in human erythrocytes during blood storage.

Changes in glycolysis control characteristics (dependence of glycolysis rate on ATP concentration) in erythrocytes were studied during the storage of donors blood with glucose citrate hemoconservant. During the first two weeks of storage the shape of glycolysis control characteristics in the erythrocytes could be shown to remain practically unchanged, which was represented by a bell-shaped curve such as in fresh erythrocytes. During this period the physiological point of glycolysis will move along the glycolysis control characteristics towards the maximum of the curve. Once the maximum of the physiological point has been reached, the shape of the curve can be seen to change. The maximum on the curve becomes less evident, moving down and to the left from its initial position. These changes will occur after two to four weeks of storage. In some cases the maximum on glycolysis control characteristics will disappear at the latest stages of storage. The changes observed will occur in blood of different donors at different moments of storage. The nature of the changes observed and their influence on erythrocyte viability are discussed.

Adenosine Triphosphate↗
PubMed1983

Preparing platelet concentrates from banked blood stored for 1-5 days by using tetracycline antibiotics.

It was shown that storage of banked blood up to five days did not change number of platelets and their functional integrity was retained rather well. That made possible the development of a method for preparation of viable platelet concentrates (PC) from stored blood. This method is based on the reversibility of the tetracycline antibiotics inhibitory effect on platelet activation process. According to the results of our in vitro studies PC from stored blood seem to be suitable for clinical usage. This tetracycline method of PC preparation from stored blood may provide a more efficient utilization of available donor blood to meet the ever-expanding needs for PC transfusions.

Blood Platelets↗