Biomedical subjects

V A Babichev

Publications and source records attributed to V A Babichev.

At least 19 recordsLinked to original sources

PubMed1978

The kinetics of inhibition with methotrexate and vinblastine of the primary immune response to sheep red blood cells in mice.

Forty-eight or 72 h after immunization with sheep red blood cells, CBA mice were injected intraperitoneally with a single dose of methotrexate or vinblastine, 2.5 mg/kg and 1.5 mg/kg respectively, and then the number of direct plaque-forming cells in their spleen was estimated at 4-h intervals. The PFC growth curve showed an exponential and staircase pattern. After administration of both cytostatics, the PFC numbers in the spleen of mice changed in a wave-like fashion: the increase was accompanied by a decrease. In some parts of the curves obtained the PFC numbers increased or decreased 2 to 7 times within 4 hours. A marked increase in PFC numbers in the controls and in animals treated with both cytostatics occurred at 60, 72--76, 84--88 h of the immune response. It is suggested that methotrexate and vinblastine do not prevent recruitment of precursor cells into PFC and consequently the number of PFC increases. The decrease in PFC numbers under the influence of both cytostatics presumably results from "unbalanced growth".

Animals↗

PubMed1977

[Immunosuppressive properties of vinblastine and colchicine].

The action of vinblastine and colchicine on the populations of antibody-forming cells, their precursors and stem cells using a model of primary immune response and in the transplantation system was studied using techniques by Jerne and Nordin (1963), Kennedy et al. (1965), and Till and McCulloch (1961). It was discovered that vinblastine and colchicine, at single administration 48 hours after immunization, inhibited immune response completely. The administration of colchicine and vinblastine to a donor gave an exponential decrease of in foci-forming cell number in a recipient in the system of syngenic transfer. But at the same time, vinblastine at small doses stimulated population of antibody-forming and stem cells, whereas at large doses it was not effective. Colchicine, in contrast, strongly inhibited both antibody-forming and haemopoietic cells in the recipient.

Animals↗

PubMed1976

[Synthesis of messenger-like RNA in plasma cels producing different clases of immunoglobulins].

Electrophoretic analysis of the nuclear poly A RNA cells of the MOPC 21, MOPC 406 and MOPC 41 plasmocytomas demonstrated a similarity in their distribution by the mol wt. Kinetics of the label accumulation in the nuclear poly A RNA of different plasma cells was unitypical. Individual peculiarities of the distribution of the cytoplasmic poly A RNA were expressed for each individual line of the myeloma cells. The majority of the molecules of the heterogenous RNA in the plasma cells were subjected to posttranscription polyadenylation.

Animals↗

PubMed1976

The effect of Trichinella spiralis on graft-versus-host reaction, transplantation immunity and antibody formation.

The effect of Trichinella spiralis in different phases of infection on transplantation immunity, the ability of lymphocytes to induce graft-versus-host reaction, on antibody and plaque-forming cell production was studied. In certain phases of Trichinella spiralis beginhing from the first days of infection and during 40 days significant suppression of transplantation immunity was observed. Thus, on the 24th day of infection skin allograft necrosis occurred much later (26.2 days) as compared with non-infected mice (12.5 days). The spleen cells of C57BL/6j mice infected with Trichinella spiralis on the 22, 29, 36, 42, 56, 72 days either induced slight graft-versus-host reaction in (CBA X C57B1/6j) F1 hybrid mice or did not induce it at all (42nd day of infection). The amount of antibody forming cells in Trichinella-infected mice significantly decreased on the 20, 25, 47, 55th days of infection. Hemagglutinin production to sheep red blood cells was significantly inhibited on the 25th day of Trichinella spiralis infection. The concept of immunodepressors being released from helminths is briefly discussed.

Animals↗

PubMed1975

Analysis of the Kinetics of the immune response by means of ionizing radiation.

The inhibitory kinetics of the primary immune response was studied by means of lethal irradiation. CBA mice were immunized with sheep red blood cells and 48, 72 and 96 hours later, they were irradiated with 750R. Afterwards, the number of direct plaque-forming cells was determined in their spleens during 58 to 66 hours at 4-hour intervals. After irradiation at 48 and 72 hours of the immune response, a plateau of different duration was obtained: 48 hours in the former and 24 hours in the latter case. In both cases, reduction in the PFC number began at about 96 hours of the resonse. The PFC reduction curves in these two series of experiments and those obtained 96 hours after irradiation and in control, non-irradiated mice were parallel. The mean half-time of the PFC number reduction as determined by these curves was 6 hours. The time of functioning of PFC in the spleen is discussed with respect to recruitment of proliferating precursors.

Animals↗

PubMed1975

[RNA biosynthesis in the microsomes of splenic cells during the inductive phase of the immunological response].

RNA labeled with 3H-uridine was extracted from the microsomes of the spleen of the intact and antigen-stimulated mice. A study was made of the composition of this RNA by electrophoresis in polyacrylamide gel. Apart from rRNA, there were revealed in the microsome composition up to 10 RNA components in the mol wt range of from 0.4-10(5) to 7-10(5) dalton and 2 componnents - between 7-10(5) and 1.7-10(6) dalton. Incorporation of 3H-uridine into the rRNA was the maximal 24 hours after the administration of the antigen, whereas the RNA with the mol wt between 0.4-10(5) dalton remaining practically unchanged for a period of three days after the immunization. 3H-uridine incorporation into these RNA was resistant to the action of low antibiotic (actinomycin D) doses. Immunization was not accompanied by the appearance of new, by molecular weight, RNA components.

Animals↗

PubMed1974