PubMed Health⌕ Search

Biomedical subjects

V A Filov

Publications and source records attributed to V A Filov.

At least 55 records · Page 3Linked to original sources
PubMed1984

[Gluconeogenesis during therapy of experimental tumors with hydrazine sulfate].

Experiments in vivo were made to examine the action of hydrazine sulfate on the gluconeogenesis in the liver and kidneys of rats with Zajdela's hepatoma and mice with lymphoma NK/Ly. The intensity of the gluconeogenesis in the liver descended and in the kidneys increased. These alterations did not affect blood glucose content in the test animals. The data obtained do not support the hypothesis that the mechanism of hydrazine sulfate action by an abrupt depression of the gluconeogenesis during malignant growth is the sole mechanism.

Animals↗
PubMed1982

[Selective action of hydrazine sulfate in combination with thiophosphamide on tumor cell mitochondria].

Hydrazine sulfate significantly potentiated antitumor effect of thiophosphamide in experiments on rats with Walker's tumor. The treatment with hydrazine sulfate (60 mg/kg) plus thiophosphamide (1 mg/kg) resulted in suppression of tumor growth up to 90%, the dosage of thiophosphamide being therapeutically ineffective. Following hydrazine sulfate treatment, the activity (pH: 7.0-7.5-7.75) derived from tumors doubled, as compared with control. Similar results were obtained with enzymatic preparations. The activities of DNP-stimulated ATPase and solubilized enzymes in rat liver were not influenced by treatment.

Adenosine Triphosphatases↗
PubMed1982

[Acid phosphatase isoenzyme composition of the lysosomes of normal and tumor cells].

Heterogeneity of acid phosphatase in lysosomes of the rat's liver and the Zhaidel hepatoma was studied by the DEAE-cellulose chromatography. It is found that different substrates are hydrolyzed by different molecular forms of the enzyme. It is suggested that variability in the increase of the enzyme activities, measured with different substrates under alteration of lysosomes, may be due to the heterogeneity of molecular forms of acid phosphatase.

Acid Phosphatase↗
PubMed1980

[Experimental study of the combined effect of 5-fluorouracil and cyclophosphane on growth of Zajdela hepatoma and nucleic acid metabolism].

Zajdela's ascites hepatoma of rats was treated with diverse combinations of 5-fluorouracil (5-FU) and cyclophosphane to study antitumour effect and the intensity of inhibiting the synthesis of nucleic acids. It was established that administration of 5-FU during the first three days and CP during the next three days in doses producing no effect when the drugs are given alone leads to an abrupt inhibition of tumour growth with concurrent inhibition of the synthesis of DNA and all fractions of total RNA. Administration of the drugs in the same succession but in higher doses that produce an inhibition of tumour growth (when the drugs are given alone) results in an antitumour action without eliciting pronounced side effect on bone marrow hemopoiesis.

Animals↗
PubMed1979

[Experimental data to substantiate the antitumor action of hydrazine sulfate].

It was found that under the influence of hydrazine sulfate, which in the dosage of 60 mg/kg would induce a 50% suppression of Walker carcinosarcoma in rats, there is the decreased activity of mitochondrial monoamine oxidase and a sharp reduction in the activity of some microsomal phosphatases. Vitamin B6 injection some hours before injecting hydrazine sulfate lessens the rate of suppression of the tumor growth up to 50%. The results obtained indicate that the antitumor effect of hydrazine sulfate may be related to the suppressed monoamine oxidate activity and vitamin B6 deficiency.

Animals↗
PubMed1979

[Quantitative aspects of the relationship of the antitumor activity and toxicity of diaziridinyl-sym. triazines to their physicochemical properties].

The experiments on rats have shown that there is a linear dependence of the logarithms of reciprocal molar toxic (LD50) and therapeutic (ED50) for sarcoma 45 doses of 6-oxyalkyl-amino-2,4-bis(I-aziridinyl)-sym-triazines and 6-(5-substituted-2,2-dimethyl-1,3-dioxan-5-yl)-amino-2,4-bis(I-aziridinyl)-sym-triazines upon the induction constants of substituents at tertiary carbon, the antitumor activity being more sensitive to the substituent effect compared with the toxicity. The dependence of the logarithms of reciprocal molar therapeutic (ED50 for sarcoma 45 and ED95 for Walker sarcoma) and toxic (LD50 and LD100) doses of the compounds, both synthesized by us and taken from the literature, upon the logarithms of their distribution coefficients in the octanol-water system (IgP) shows a parabolic character with the maximum effect at the extreme point of the parabola. The antitumor activity was more sensitive to a changed IgP than the toxicity.

Animals↗
PubMed1978

Effect of chemotherapy on the lymph node and bone marrow cell chromosomes in patients with Hodgkin's disease.

Chromosomal aberrations in bone marrow and lymph node cells have been investigated in 47 patients with lymphogranulomatosis 24--72 hr after administration of a single therapeutic dose of cyclophosphan and natulan. It is shown that the injurious action of cyclophosphan and natulan lasts more than 72 hr. An analysis of the dynamics of various types of chromosomal aberrations testifies that cyclophosphan and natulan affect cellular constituents found in the synthetic or post-synthetic phases of the cell cycle.

Adolescent↗
PubMed1977

[Effect of 5-fluorouracil and thiophosphamide on the activity and fractional content of phosphatases of Zajdela hepatoma].

The administration of different types of antitumor drugs is found to lead to a decrease in total activity of phosphatases in mitochondrial, lysosomal and microsomal fraction of Zajdela hepatoma cells, the free activity being enhanced. The isoenzyme pattern of phosphatases in soluble fraction is influenced too. The results suggest that different antitumor drugs cause a uniform response in tumor cells, manifested in disorders in the protein synthesizing system and membranes of subcellular particles involved.

Adenosine Triphosphatases↗
PubMed1977

[Mechanism of increasing the effect of antineoplastic agents by hydrazine sulfate].

The results of the in vivo and in vitro experiments indicated that hydrazine sulphate is a new potent inhibitor of the biotransformation of physiologically active compounds, including those with an antitumor effect. It is suggested that hydrazine sulphate renders an inhibitory effect by retarding the access of xenobiotics to microsomal enzymes active centers metabolizing them. The retardation of antitumor compounds biotransformation effected by hydrazine sulphate in vitro enabled the explanation of the enhanced effect of cytostatics, used in combination with hydrazine sulphate in vivo. This fact seems to speak in favour of the perspective clinical application of hydrazine sulphate for potentiation of the antitumor effect of cytostatics.

Animals↗