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V A Golichenkov

Publications and source records attributed to V A Golichenkov.

At least 19 recordsLinked to original sources
PubMed1991

[Use of high velocity mechanical injection for the transfer of foreign DNA into early mouse embryos].

The possibility of high velocity mechanical transfer of foreign DNA into inner cell mass of mouse blastocyst was shown. Penetration of tungsten microparticles into early embryo cell nuclei and their localization on mitotic chromosomes was demonstrated. About 70% of developing embryos survived the bombardment. Total DNA of the mice born from bombarded embryos was analyzed by blot-hybridization and PCR with Southern hybridization. In three cases, the presence of the transferred plasmid DNA (pSV3-neo) was revealed.

Animals↗
PubMed1984

[Pigmentation of the outer cornea after removal of the eye from Rana lessonae Camerano larvae].

Dynamics and morphological peculiarities of pigmentation in the external cornea after removal of the eye in larvae of Rana lessonae Camerano have been studied in situ depending on the developmental stage during prometamorphosis. The results are compared with the previously obtained data for tadpoles of Rana temporaria (D. V. Popov, Sokolova Z. A., Kalistratova E. N. et al., 1982). The process of pigmentation in the cornea does not depend on the developmental stage, proceeds with a constant rate and accomplishes before the beginning of the metamorphous climax. In the Rana lessonae tadpoles the dermal chromatomorphous units differentiate earlier than in the Rana temporaria larvae. The period of metamorphosis in the R. lessonae is 2 times as long as in the R. temporaria. At the same time, transplantation of the epidermal melanophores during the process of the cornea pigmentation in these species is realized with the same rate. Thus, in this case, the phenomena occurring at the cellular and organism levels are rather autonomous. The differences revealed between the cornea pigmentation in the R. lessonae and R. temporaria larvae are explained as the phenomenon of interlevel heterochrony in ontogenesis.

Animals↗
PubMed1983

[Possibility of using the larvae of anuran amphibians as a test model for the quantitative determination of hypophyseal hormones].

A possibility to use the larvae of ecaudate amphibia as a test model for the quantitative determination of certain hypophyseal hormones has been shown. The physiological reactions of the pigmentary cells in tadpoles of Rana temporaria can be used for a quick and exact testing of the adrenocorticotropic hormone preparations (ACTH): corticotropin and Zn-corticotropin. Evidently the method elaborated for the biological testing of hypophyseal hormones may be used in the production of the endocrinous preparations as a quick assessment of melanocyte-stimulating hormones in integral raw and sublimated hypophyses.

Adrenocorticotropic Hormone↗
PubMed1982

[Relations between pigmentation of the cornea and the number of epidermal melanophores in Rana temporaria L. larvae].

The dynamics of the external cornea pigmentation in Rana temporaria L. larvae at the 22d developmental stage have been studied under conditions favourable for various course of certain morphological reactions in the pigment system. The cornea together with the surrounding skin is transferred on the dorsal surface of the larva body, and the piece of the dorsal surface skin is put instead of the cornea removed. When using the reciprocal transplantation method and preserving the organism's integrity (without disturbing melanocyte-stimulating source--namely, the hypophysis, and melatonine sources--namely, the pineal gland and the lateral eyes) the corneal pigmentation is observed on the background of perfect morphological reactions in the pigment system, while the larvae are maintained on the dark and light substrates, that is at various density of the pigment cells (120 larvae have been used). The pigmentation dynamics have been studied from the 6th up to the 20th day in total preparations. The epidermal melanophores density is estimated in 4 areas of each preparation. The melanin amount is estimated by means of the electron paramagnetic resonance-spectrometry according to the contents of free radicals expressed in relative units. A direct proportional dependence between the significantly higher melanin contents (1.5-fold) and a significantly quicker (1.5-fold) process of the corneal pigmentation is revealed, that agrees with an increasing number of the pigment cells per one unit of the body surface in the larvae maintained on the dark substrate. In the larvae maintained on the light substrate, the dependence is of a reverse character. It is probable that the factors forcing the pigmented cells, at cultivation the neural crest cells in vitro to reject from each other, affect the pigmentation of the larval cornea in vivo. If it is the case, the processes specific for the embryonal period, transgress during the cornea pigmentation at the larval stages of development.

Animals↗
PubMed1982

[Corneal pigmentation after removal of the eye in Rana temporaria L. larvae].

Pigmentation of the cornea has been studied in the Rana temporaria larvae at the 22d-27th stages of development macroscopically (total corneal preparations with the surrounding skin) and microscopically. Dynamics of the pigment cells transfer that fill the transparent cornea is compared to the histological rearrangement of the latter. The corneal pigmentation is demonstrated to occur with a constant velosity and is well described by means of the linear regression equation. At initial stages of transformation, the corneal pigmentation overtakes the histological processes, then the two processes run in parallel and only towards the metamorphosis period the corneal histological changes slightly overtake the pigmentation process. As a whole, the pigment cells arrangement demonstrates the transformation degree of the external corneal into the skin after the eye has been extirpated. A suggestion is made explaining the necessity of a constant inducing retinal influence on the skin in Anura larvae.

Animals↗