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Biomedical subjects

V A Gritsenko

Publications and source records attributed to V A Gritsenko.

At least 19 recordsLinked to original sources
PubMed2006

[Taxonomic spectrum and antibiotic resistance of Enterobacteriaceae isolated from bile of patients with cholangitis].

The data on the taxonomic structure and antibiotic resistance of Enterobacteriaceae isolated from bile of patients with various clinical variants of cholangitis are presented. It was shown that bacteriocholia was registered in 53.3-90.9% of the patients during operations and in 88.9-100% of the patients during the postoperative period. Among bilicultures enterobacteria dominated (93.4% of the cases) with predominance of E. coli and Klebsiella spp. (the total about 70%). The enterobacteria isolates were frequently resistant to ampicillin and amoxycillin/clavulanate (92.6 and 70.4% of the strains respectively), gentamicin and amikacin (74.1 and 22.2%), cefazolin and cefotaxime (88.9 and 37.0%). The resistance to imipenem and ciprofloxacin (7.4%) was rare, that should be considered while prescribing drugs for empirical antimicrobial chemotherapy.

Adult↗
PubMed2005

[Taxonomic characterization and antibiotic resistance of pathogens of perinatal infectious inflammatory pathology in infants in Orenburg].

The materials on regional features of the nosologic structure, taxonomic spectrum and antibiotic resistance of the pathogens of perinatal infectious inflammatory pathology (IIP) in infants in Orenburg are presented. From 2002 to 2005 the average level of IIP of 19.0 cases per 1000 living new borns and the dominance of skin inflammatory diseases (vesiculopustulosis in particular) in the nosologic structure among the other forms of IIP (including conjunctivitis and pneumonia) were observed. The prevalence of the gram positive cocci with the leadership of coagulase negative staphylococci among the skin and eye forms of IIP was shown. Their portion reached 62.8%. The portion of the other microorganisms was much lover. Gramnegative bacteria (Neisseria spp., E. coli, K. ozaenae) were isolated from 6.1, 4.3 and 0.6% of the cases. The Candida isolates were detected in 1% of the cases. The regional antibiotic resistance register for the pathogens of IIP was developed. Its use is obligatory in prescribing drugs for empirical therapy of such pathology and prevention of its complications.

Bacteria↗
PubMed2000

[The in-vitro effect of the leukocytic cationic protein preparation Intercide on Escherichia coli].

Intercide is a cationic protein with the molecular weight of 11.0-11.5 kD from human leukocytes. The in vitro effect of its different concentrations (0.6 to 1.8 mg/ml) on populations of Escherichia coli M17 and K12 and 120 E.coli isolates from various sources such as water, feces of healthy humans and patients with extraintestinal escherichiosis was studied. The experiments with the bacterial suspensions and broth cultures demonstrated that Intercide had an antibacterial action on both the stationary and growing cells. However, some strains of E.coli were resistant to the lethal effect of Intercide. It was observed for the first time that in a concentration of 1.8 mg/ml Intercide was able to stimulate the biomass growth of some E.coli strains in broth culture. The factor analysis showed that the Intercide stimulating effect was more often evident with respect to extraintestinal escherichiosis pathogens with high anti-Intercide and antilysozyme activities.

Anti-Bacterial Agents↗
PubMed1997

[Role of intraspecific phenotypic diversity in the ecology of escherichia coli and staphylococcus aureus].

Investigating a complex of biological characteristics, including the inactivating ability of some antiinfectious resistance agents (lysozyme, complement, immunoglobulins, the bactericide component of interferon) in 229 and 257 E. coli and S. aureus strains, respectively, isolated from various sources has revealed the phenotypical polymorphism in the populations of these microorganisms, whose degree and specific features may be characterized by the indices of biological diversity and by the spectra of dominant biological profiles. Interpopulational variability in the bacteria was found to be determined by the specific features of their colonized ecotopes and to reflect the level of their adaptation to their inhabitance. There is a view of the organizational structure of the bacterial species as a whole complex of discrete populations of microorganisms, which include representatives of phenotypically different clone lines that occupy the optimum and some marginal econiches whose relation is supported by migration processes.

Adaptation, Biological↗
PubMedDate not supplied

[Phasic changes in reproductive potential, intracellular polyamines and antilysozyme activity in Escherichia coli in periodic culture].

Growth-phase associated changes in and relationships between the specific growth rate (mu) characterizing the reproductive capacity of the cells, the contents of intracellular biogenic polyamines (BPA), such as putrescine (P), cadaverine (C), and spermidine (S), and antilysozyme activity (ALA) were studied in 37 strains of Escherichia coli grown in batch culture on solid medium. A decrease in mu upon the transition of the culture to the stationary growth phase was accompanied by a decrease in the pool of free BPA, mainly P and C, and by the appearance of ALA. The interrelations between the parameters studied were described as a complex of direct and negative correlations; the combination of low initial P and C contents, reduced P/S and C/S ratios, and a high level of ALA was designated as a factor of slight inhibition of E. coli reproduction. It is argued that BPA and ALA are integrated in a system controlling both the metabolism and stability of peptidoglycan in E. coli.

Biogenic Polyamines↗
PubMedDate not supplied

[Nonenteric escherichiosis and the problem of human reproductive health].

The contribution of nonenteric Escherichia infections (NEI) to the formation of male and female infertility is analyzed. The negative influence of NEI, and particularly pyelonephritis, on the course of pregnancy, parturition and the postnatal period is shown. The role of E. coli in the development of life-threatening meningoencephalitides, pneumonia, sepsis in newborn infants is emphasized, NEI risk factors are systematized with due regard to concrete stages in the pathogenesis of this pathology. Phenotypic differences between the causative agents of NEI and nonpathogenic and diarrhea-inducing E. coli are characterized.

Chronic Disease↗
PubMedDate not supplied

[The seroresistance of enterobacteria isolated from different sources].

395 representatives of the family Enterobacteriaceae, isolated from household and drinking water, from healthy and sick persons, were used as an example to demonstrate that their seroresistance was greatly connected with the type of the source from which these bacteria had been isolated. Experiments showed the phenotypic conversion of Escherichia coli towards an increase in their seroresistance on contact with human blood serum. The study revealed the pleiotropic effect produced by the action of serum on a number of other properties, responsible for survival in a macroorganism (anticomplement and antilysozyme activities, adhesive capacity and hydrophobic properties of the outer surface).

Bacteriuria↗
PubMedDate not supplied

[The properties of Escherichia isolated from the bodies of mice in bacterial translocation after immobilization stress].

As revealed in experiments on mice, 6-hour immobilization stress initiates the process of the translocation of intestinal flora to mesenteric lymph nodes and the blood. This process is accompanied by the infection of parenchymatous organs (the liver, the spleen, the kidneys, the lungs) and the increase of the concentration of E. coli in the proximal sections of the digestive tract (the duodenum and the jejunum). As the result of the comparative analysis of the phenotypic signs of bacterial isolates obtained from intestinal and "extraintestinal" E. coli populations, the accumulation of clones with highly pronounced seroresistance and such persistence characteristics as anticomplementary and antilysozume signs, as well as resistance to the bactericidal action of leukocytic cation protein with a molecular weight of 11.0-11.5 kD, has been found to occur in the body (the blood, parenchymatous organs and the small intestine).

Animals↗
PubMedDate not supplied

[The ecological determinacy of the intraspecies diversity of pathogenic bacteria].

The review deals with some theoretical and applied problems of the intraspecific diversity of pathogenic microorganisms. Special attention is given to the role of ecological factors in the formation of the phenotypic polymorphism of bacteria. The possibility of using the methods of mathematical analysis for the evaluation of the influence of the environment (ecotopes) on individual phenotypic characteristics of bacteria and their complex (bioprofile) is shown. The proposition on the bioprofile of microorganisms as a criterion of their ecological (ecotopic) marking is substantiated. The examples of using the data of the ecological marking of pathogenic bacteria in clinical epidemiology and ecological practice are presented.

Bacteria↗
PubMedDate not supplied

[The ecological and medical aspects of the symbiosis between Escherichia coli and man].

In this work different variants of the symbiosis of E. coli with a human body are analyzed, and the symbiotic relationships between them are shown to follow the type mutualism, commensalism, parasitism and habitation. The authors emphasize that the multiplicity of variants of bacteria-host relationships is based on the phenotypic polymorphism of E. coli clones (clone lines). Taking into account their ecological (symbiotic) features and biomedical importance, all E. coli clones are divided into 4 groups (clusters): mutualists as nonpathogenic organisms; commensals as potential pathogens (causing extraintestinal E. coli infections); parasites as real pathogens (causing acute intestinal infections); "occasional" symbionts of man. The proposition on the cluster structure of E. coli as a species is formulated.

Bacterial Translocation↗
PubMedDate not supplied

[Ecovariant differences of Escherichia coli strains with respect to colicinogenicity and colicin resistance].

The analysis of 173 Escherichia coli strains, isolated from different sources, for colicinogenicity and colicin resistance revealed that frequency of these signs increased in the following order: water in open reservoirs, intestine, extraintestinal localizations. In most cases resistance to 5 or more bacterial colicins was due to the absence of the corresponding receptors to colicins. Colicin resistance and colicinogenicity render E. coli selective advantages under the conditions of intestinal microbiocenosis.

Child↗
PubMedDate not supplied

[Characteristics of enterobacteria isolated from patients with urogenital pathology].

Species composition and a number of persistence characteristics enterobacteria isolated from urine of 42 pregnant and 22 nonpregnant women with pyelonephritis (relapse, remission), from prostatic fluid of 225 males and secretions of cervical canal of 124 women with urogenital pathology (prostatitis, salpingo-oophoritis) were studied. The study revealed that enterobacteria, including Escherichia coli, prevailed in the structure of uromicroflora (66.7-83.3%) and constituted a relatively small proportion among "genital" isolates of microorganisms (19.9-22.2%). Male and female sterility and the presence of enterobacteria in the reproductive tract of patients were found to be directly correlated. Clinical isolates of enterobacteria were shown to possess pronounced seroresistance and the complex of persistence characteristics, including antilysozyme, anti-intercidal and anticomplementary activity.

Adolescent↗
PubMedDate not supplied

[Comparative analysis of the sensitivity of enterobacteria to bile].

The analysis of the sensitivity of 195 enterobacterial cultures to bile revealed that their level of resistance decreased in the following row: Shigella > Salmonella > Klebsiella > Escherichia > Providencia. As shown on a sample of 136 E. coli isolates the level of resistance of these bacteria to bile depended on their isolation source: in E. coli isolated from bile in cholecystitis, from urine in pyelonephritis and from feces in intestinal dysbacteriosis resistance was 1.1-1.3 times higher than in E. coli isolated from the water of open reservoirs, from the feces of healthy persons and from extraintestinal foci of purulent inflammation. The level of sensitivity to bile is regarded as a property making it possible for enterobacteria to colonize biliary tracts and the proximal sections of the digestive tract.

Bile↗
PubMedDate not supplied

[Resistance of Escherichia coli to "Intercid", a leukocyte cation protein].

In vitro experiments on 120 E. coli strains isolated from different sources (43 from water, 22 from the feces of healthy persons, 55 from patients with extraintestinal E. coli infections) have demonstrated that some of E. coli isolated, mainly the causative agents of extraintestinal infections, are resistant to the antibacterial action of "intercid" and have anti-Intercid activity (AIA) and a high level of antilysozyme activity. The use of the correlation and two-factor analyses has made it possible to establish the relationship between these characteristics and to find out an even more important contribution of AIA to the determination of bacterial Intercid-resistance (fusion force h2 = 13.1-25.0%). Experiments on E. coli isogenic variants with phenotypes AIA+ and AIA- have shown that AIA is an additional factor which determines the resistance of E. coli to "intercid".

Anti-Bacterial Agents↗