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V A Guliaev

Publications and source records attributed to V A Guliaev.

51 records · Page 3Linked to original sources
PubMed1985

[Relative biological effectiveness of highly active mixed gamma-neutron 252Cf radiation].

Comprehensive radiobiological studies of the relative biological and genetic efficacy (RBE and RGE) of powerful 252Cf radiation (the ANET-B unit) were conducted using research tools of various radiosensitivity (bacteria, Drosophila, Chinese hamster cells, murine thymocytes, human and murine bone marrow stem cells, human peripheral blood lymphocytes, Lewis lung carcinoma cells). It was shown in the tests of reproductive or interphase death and chromosome aberrations that the RBE and the RGE values of a 252Cf new source varied within the same limits from 1.3 to 3.0 whereas in the tests of gene mutations the RGE of the source did not exceed the efficacy of 60Co gamma-radiation and in some cases it was much lower. Thus the RBE of the new source in induced lethal and chromosome damages was 2-4 times lower than the efficacy of a low-activity 252Cf source used now in radiotherapy.

Animals↗
PubMed1978

[Early death of pancreatic A-cells after irradiation with supralethal doses].

For investigation of postirradiation reactions, of pancreatic insular cells, male Wistar rats were subjected to 1.5, 3, 7, and 15 kR of Co60 gamma-radiation. It was found that after application of doses of 7 kR or higher, interphase death of all the insular A-cells occurred within 6 hours. The cell death was demonstrated to be characterized by chromatin pycnosis, dilatation of the endoplasmic reticulum and perinuclear space, mitochondrial swelling, and desintegration of A-granules. Macrophages took no part in resorption of the dead cells. A-cell necrosis was not accompanied by morphologically observable changes in acinar cells or insucar B-cells. Some possible explanations of the more pronounced radiosensitivity of A-cells are discussed.

Animals↗
PubMedDate not supplied

[The effect of chronic butyphos poisoning on protein and nucleic acid synthesis in various rat organs and activity of blood cholinesterase].

Two doses of butyphos (1/10 and 1/50 LD50) were administered into rats within 6 months. Content of DNA was distinctly decreased in liver and kidney tissues within the first 2 weeks of the pesticide administration. Then DNA synthesis was increased 2-fold in liver tissue and remained high during all the 6 months of intoxication. Protein synthesis was increased in liver tissue within 3 months of the administration and remained elevated up to the end of experiment. High rate of protein synthesis, found in kidney and spleen tissues at the initial steps, was markedly decreased within 6 months. Content of DNA and RNA was decreased in the tissues studied within 1 month of the intoxication and restored within 3 months, while it remained at considerably lower level in liver and spleen tissues as compared with control values. Cholinesterase activity was lowered by 90% in blood within 11 weeks with the subsequent increase; but in the experimental group intoxicated with butyphos at 1/10 LD50 the enzymatic activity constituted only 60% of control values within 6 months. Histological study showed development of necrodystrophy in liver tissue and of fibroplastic glomerulonephritis in kidney. The deteriorating effect of butyphos on cellular genome functions appears to relate not only to its cytotoxicity but also to the cancerogenic and mutagenic properties of the pesticide.

Animals↗