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V A Ivanova

Publications and source records attributed to V A Ivanova.

At least 19 recordsLinked to original sources
PubMed1998

[EGF-dependent association of 20S-proteasome and alpha-RNP particles with the epidermal growth factor receptor in A-431 cells].

Here we demonstrate that the epidermal growth factor (EGF) induces association of prosomes (20S-proteasomes) with its receptor in A-431 cells. Additionally, ligand-dependent association of ribonucleoprotein particles (alpha-RNP), containing small ALU-like RNA, with the EGF receptor was demonstrated. A suggestion has been put forward on the involvement of prosomes and alpha-RNP in the EGF signal transmission to different stages of gene expression.

Cysteine Endopeptidases↗
PubMed1996

[The use of the antihypoxants olifen and amtizol for treating patients with chronic stage-IIB circulatory failure].

To compare efficacy of conventional therapy, antihypoxants olifen and amthizol (groups 1, 2 and 3, respectively) 49 patients with congestive heart failure stage IIb according to the Vasilenko-Strazhesko classification were treated. In hospital the pre-hospital scheme of treatment was not changed except the following positions: group 1 patients received strophanthin instead of oral digoxin, group 2 and 3 patients received adjuvant olifen and amthizol, respectively. Stabilization of the condition occurred in 10 days, on the average, in each group. In the 1st group good results were obtained due to stroke volume increase, in the 2nd and 3d due to the ability of olifen and amthizol to prevent tissue hypoxia.

Antioxidants↗
PubMed1988

[Radiometric detection of nonvisualizable immunoprecipitates formed by hepatoma-associated hetero-organic renal antigens of 33P-phosphorylated fractions of non-histone chromatin proteins].

A study was made of the antigenic properties of nuclear nonhistone proteins (NHP) from rat tissue eluted with 0.4-0.5 M NaCl gradient on phosphocellulose at very low concentrations. 33P-labeled proteins were obtained by phosphorylation of NHP fractions in the presence of [gamma-33P] ATP. Some non-visual precipitates which appeared during the reaction of specific inhibition of precipitation in agarose gel of 33P-labeled NHP-fractions from rat solid hepatoma 27 cells or rat liver after single hepatocarcinogen diethylnitrosamine injection were detected by means of a calculable radiometric method, proposed by the authors. These precipitates appear only if the rabbit immune sera against NHP-DNA complexes of intact rat kidneys exhausted with liver NHP-antigens was used, never appearing in the case of kidney NHP-antigen exhaustion. Therefore the NHP-fractions prepared from "carcinogenic" liver and rat solid hepatoma cells which possess proper phosphoprotein kinase activity and form specific immune precipitates must be identified as hetero-organic antigens of kidney origin.

Adenosine Triphosphate↗
PubMed1985

[Multicomponent detoxifying system in albino rat liver].

Experiments on white rats were made to study the system of xenobiotic detoxifying. The measurements were taken of the content of cytochromes P-450 and b5, of the activity of NADP X H- and NAD X H-dependent monoxygenases, of the levels of microsomal albumin, thiol groups, and of the mass index of the liver in intact animals and in those injected with phenobarbital and SKF-525A. The most informative indicators determining the detoxifying system functioning were identified by factor analysis. Correlations were also established between components of the system. It turned out that transformation of the correlations in question was the result of phenobarbital and SKF-525A injection. The authors discuss the importance of the character and degree of this transformation for evaluation of the tension of the xenobiotic detoxifying system functioning and measures aimed at ensuring its reliability under the effect of harmful environmental factors.

Animals↗
PubMed1981

[Correlation between hematologic and biochemical indices of Testudo horsfieldi turtles].

The morphological composition and some of biochemical factors of turtle blood, as well as correlation between the former and the latter were examined. It was revealed that the turtles and mammals differed in the factors under consideration. The data obtained are interpreted in the light of the formation of the organism's response to the environmental exposures.

Animals↗
PubMedDate not supplied

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Cell Extracts↗
PubMedDate not supplied

[Cloning and expression in Escherichia coli of reverse transcriptase coded by the mobile genetic element jockey].

The mobile element jockey is similar in structural organization and coding potential to the LINEs of various organisms. Current models of the mechanism of transposition involve reverse transcription of an RNA intermediate and utilization of element-encoded proteins. As it is demonstrated here, a 2.23 kb DNA fragment from the region of the jockey encoding the putative reverse transcriptase, was stably introduced into the expression system under inducible control of the Escherichia coli lac regulatory elements. We describe the expression of the 92 kDa protein and identify this polypeptide alone as authentic jockey reverse transcriptase based on some of its physical and enzymic properties. The jockey polymerase demonstrates RNA-directed and DNA-directed DNA polymerase activities, but lacks detectable RNase H, has a temperature optimum at 26 degrees C, requires Mg2+ or Mn2+ as a cofactor and is inactivated by sulfhydryl reagent. The enzyme prefers poly(rC) and poly(rA) as template and "activated" DNA is not effective. The results of this work suggest that the RNA-directed DNA polymerase coded by jockey elements may be involved in the transcription of the elements.

Amino Acid Sequence↗