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Biomedical subjects

V A Korolev

Publications and source records attributed to V A Korolev.

At least 19 recordsLinked to original sources
PubMed2005

[Estimation of glycated hemoglobin values as a mathematical model].

By using various assays of glycated hemoglobin, the author deduced a model of this index by its level, association with major metabolic parameters from the linear and nonlinear correlations and regression. The duration of diabetes mellitus, serum total cholesterol, and postprandial glycemia were found to have the most considerable impact on the formation of different models of this index.

Adolescent↗
PubMed2004

[Strategic approach to glycohemoglobin determination].

The purpose of the case study was to design an approach involving the clinical use of glycated hemoglobin (GH) in patients with diabetes mellitus (DM). 139 patients with DM, types 1 and 2, were examined. GH was quantified by 4 methods, i.e. immunochemistry, colorimetry, by oxalic and phosphoric acids and by isoelectrofocusing. It was revealed by correlation and regression assays that the GH level made a non-linear bond and depended to a great extent on a daily glucosuria, daily proteinuria, total serum cholesterol, body weight index and disease duration. Whereas no such bond was found for accidental pre- and postprandial glycemias. The conclusion is that, with due respect to a comparative analysis of different techniques applicable to GH quantification, the discussed index still remains a reliable criterion of the profound metabolic conditions, and that argumentative methods must be used in its quantification.

Adolescent↗
PubMed2002

[Comparison of determination methods and application of a computer program using a screening coordinate system to interpret hemoglobin A1c in patients with diabetes types 1 and 2].

The aim of the work is to compare methods of hemoglobin A1c determination in ratio with basic metabolism indices using computer program with graphic interpretation of results in patients with diabetes of 1 and 2 types. It was found out that more specific hemoglobin determination by isoelectrofocusing method more completely reflects the state of carbohydrate metabolism in diabetic patients. The importance of hemoglobin A1c determination in case of 2 type diabetes is particularly emphasized.

Adolescent↗
PubMed2001

[Evaluation of colorimetric method for analysis of glycated hemoglobin].

The level of glycated hemoglobin (GH) measured by the Diabetic test colorimetric method did not depend on the compensation. On the other hand, its level was increased in diabetes mellitus decompensation in comparison with subcompensation, if isoelectrofocussing of the studies hemolysates was carried out prior to colorimetry. Study of colorimetric migration of hemolysates showed that they were represented by a background which could distort the results.

Calorimetry↗
PubMed1998

[The use of electrophoretic methods for determining modified proteins in diabetic patients].

Modified proteins were determined by isoelectric focusing in borate-polyol system with subsequent colorimetry (micromethod) and electrophoresis of blood serum on paper with subsequent TCA-ethanol treatment. Increased levels of glycated hemoglobin and modified albumin and changed light absorbance of glycated albumin were detected. The levels of glycated hemoglobin assessed by the micromethod and colorimetry without calibration did not correlate.

Blood Protein Electrophoresis↗
PubMed1991

[Use of 250 W YAG-Nd laser in endoscopic surgical treatment of cancer patients].

The clinical use of a fiber-laser device based on continuous 250-W YAG-Nd laser allows the pathological tissue to be treated by exposure in various energy regimens, from its coagulation to intensive evaporation; the conditions of treatment created in this case for the patient are more comfortable than those with the use of less powerful laser devices. The elaboration and manufacturing of a new design for guiding the beam into a fiber wave guide with a light-guiding lead of 600 and more microns in accordance with the model of the used endoscope even without gas injection ensures reliable functioning of the fiber-laser device. Overheating of the in put and out put ends of the fiber wave guide in exploitation at large power regimens is excluded despite soiling of the distal end of the wave guide which is inevitable during the operation.

Aluminum↗
PubMed1989

[Glycosylated hemoglobin (HbA1c) in blood in impaired tolerance to glucose and sugar diabetes].

Isoelectric focussing in borate-poliol system is employed for measuring the blood glycosylated hemoglobin (HbA1c) levels at the treatment and prophylaxis institutions in Yalta. Examinations of normal subjects and patients with disordered glucose tolerance and apparent diabetes mellitus have revealed a significant elevation of HbA1c level in the patients with impaired glucose tolerance vs. normal subjects; these levels have been virtually the same in the diabetics and patients with impaired glucose tolerance. Over the course of health resort treatment the diabetics develop negligible changes in the HbA1c levels, paralleled by a significant reduction of glycemia on an empty stomach.

Diabetes Mellitus↗
PubMed1989

[Effect of catamine AB on the generative function and chromosome apparatus of the bone marrow cells in white rats].

In a chronic experiment (6 months) performed on non-inbred white rats effect of cationic superficially active substance catamin AB on the structure and function of male and female gonads, state of the offspring and on the chromosome apparatus of the bone marrow cells has been studied. The preparation is intragastrically injected in doses 10, 1, 0.1 mg/kg. Catamin AB in the dose of 10 mg/kg produces an essential decrease in the specific mass of the testes, reduce in the spermatogenic index, increase in the canaliculi number with exfoliated epithelium, lowered mobility and diminished amount of alive spermatozoa. In the ovary amount of mature follicles becomes less and the number of atresic corpuscles and atresing follicles rises; plethora of the medullary substance occurs in the organ with signs of stasis in the venous bed. In the dose 1 mg/kg catamin AB results in less pronounced changes in the testes and does not produce any pathological disturbances in the ovaries. The preparation does not influence on mating in the test animals, but in the dose 10 mg/kg results in a decreasing fertility because of pre- and postimplantational death of embryos. The changes mentioned are connected with a total toxical effect of the preparation. Catamin AB does not possess any mutagenic effect in the doses tested.

Animals↗
PubMed1978

[Analysis of the steroid hormones in pig blastocysts].

The steroid hormonal activity was established in the pig blastocysts by means of histochemical methods. This is supported by the presence of hormones (pregnenolon, pregnandion), activity of 3beta-ol steroid dehydrogenase, high content of lipids (sterols) in the trophoblast. The results of thin-layer chromatography on silicagel suggest that the qualitative composition of lipids of the early embryos and the yellow bodies of the ovaries at the corresponding stage of pregnancy is the same.

3-Hydroxysteroid Dehydrogenases↗
PubMed1976

[Ovum lipids of placental mammals].

Morphocytochemical peculiarities of lipids from oocytes of some mammals and man were studied. The human oocytes, according to the structure and content of yolk, bear a close relation to a hare and a rabbit much differing from gametes of predatory animals (dog, cat), artiodactyla (sow), rodents (golden hamster). By thin layer chromatography on silicagel, a detailed lipid composition was established in the rabbit's oocytes, with neutral lipid and phospholipids (kefalin, lecitin, sfingomyelin) dominating. In addition, cholesterin was found.

Adolescent↗