Structure and regulation of the gene encoding avian inhibitor of nuclear factor kappa B-alpha.
The Rel/NF-kappa B family of transcription factors exist in the cytoplasm as inactive complexes in association with an inhibitory protein called I kappa B-alpha. We have isolated a clone containing the avian I kappa B-alpha gene from a chicken genomic library. Avian I kappa B-alpha is devoid of any recognizable promoter elements, i.e., TATA and CAAT boxes; however, the 5'-UTR of the gene contains the initiator elements frequently found in TATA-less genes. Avian I kappa B-alpha contains seven putative Rel/NF-kappa B binding sites. A CAT reporter construct containing the 5' upstream region of I kappa B-alpha was expressed when transfected into cells which produce I kappa B-alpha. This construct, however, was not expressed in cells in which I kappa B-alpha activity was not induced, indicating that the regulatory elements which promote I kappa B-alpha expression are contained within 1000 nt of the transcription start site. Southern analysis suggests that I kappa B-alpha is present as a single-copy gene per haploid genome and is expressed in avian hematopoietic tissues, as well as lymphoid cells transformed by avian reticuloendotheliosis virus (REV-T).