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Biomedical subjects

V A Liashenko

Publications and source records attributed to V A Liashenko.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

[Immunodepressive effect of measles and parotitis virus in vitro].

Mechanisms of suppressive effect of measles and parotitis virus on lymphocyte proliferation and cytolytic activity of natural killers are studied in vitro. Both viruses exert a weak mitogenic effect on human blood lymphocytes and at the same time suppress their response to phytohemagglutinin stimulation in vitro. Suppressive effect of measles virus is more expressed than of parotitis virus. Moreover, measles virus suppresses lysis of human blood killer target cells. The suppressive effect manifests as early as in 1 h and increases after 3 h of natural killer cell contact with measles virus.

Cell Division↗
PubMedDate not supplied

[Intranasal revaccination of children with live measles vaccine: development of local immunity].

Thirty-eight children aged 6-7 years were intranasally revaccinated against measles. Children without antimeasles antibodies in the serum responded to revaccination by their appearance in protective titers. In children with antibodies their titers increased (in 60%) on day 30 after intranasal revaccination. The efficacy of subcutaneous revaccination was the same. Intranasal, but not subcutaneous, revaccination resulted in appearance of nasopharyngeal anti-measles IgA. Intranasal method of revaccination against measles is convenient and safe and is recommended for clinical trials on a wide scale and for introduction into practice.

Administration, Intranasal↗
PubMedDate not supplied

[The determination of measles antibody titers in the latex agglutination reaction].

A new latex agglutination test (LAT) for detection of antibodies to measles virus in blood sera was developed. The latex diagnosticum consists of polymeric microspheres of national make carrying on their surface covalently bound measles virus antigen. The data on the specificity and sensitivity of the test are presented, and the results of titrations of blood sera from schoolchildren by traditional HI test and LAT are compared. LAT was shown to be highly sensitive, much simpler than the available methods for detection of measles antibodies and more economic, and therefore may be recommended for mass surveys.

Animals↗
PubMedDate not supplied

[A trial of the intranasal vaccination of adults against measles: the approaches to an alternative revaccination method].

The safety and efficacy of a measles vaccine from the L-16 strain was studied after its intranasal administration to adult volunteers using an atomizer. The controls were adult volunteers given one dose of the vaccine subcutaneously. The measles vaccine given intranasally was used in the same or double dose. The intranasal administration of the vaccine caused practically no side effects. Within 14-60 days postvaccination, the volunteers in all the groups showed a significant rise in titres of antibodies to measles virus determined by hemagglutination inhibition test. The double dose of intranasally administered vaccine was approximately as effective as a single dose given subcutaneously.

Administration, Intranasal↗
PubMedDate not supplied

[Effect of myelopeptide 2 on the immunization of guinea pigs with live measles vaccine].

A model is suggested to study the effects the myelopeptide 2 (MP2) immunomodulator on the results of live measles rash immunized by vaccine. Guinea pigs were found to respond to live measles vaccine L-16 the way man does, i.e. the antibodies' level in their sera was increasing until days 28-56 after a single intramuscular vaccine injection. An intramuscular injection of the MP2 immunomodular, made alongside with vaccination, was demonstrated to enhance the immune response to the measles vaccine and to induce its action. Finally, it was for the first time, that the possibility was proven to enhance the action of the live measles vaccine by an immunomodulator.

Animals↗
PubMedDate not supplied

[Testing for the safety, reactogenicity and antigenic properties of a live thermostable mumps vaccine made from the Leningrad-3 strain].

A live mumps vaccine (LMV) from strain Leningrad-3 with a new stabilizer LS-18 was tested for reactogenicity and antigenic potency. Examinations of vaccinated children for vaccination reactions showed its complete areactogenicity and safety. LMV induced synthesis of virus-neutralizing antibodies in 78-82% of the vaccinees. Determinations of the dynamics, intensity and duration of circulation of specific antihemagglutinating, antineuraminidase and virus-neutralizing antibodies demonstrated marked antigenic potency of the LMV and established production of earliest specific antineuraminidase antibodies in 75% of the vaccinees. EIA was found to be the most sensitive test.

Antibodies, Viral↗
PubMedDate not supplied

[The specific activity and immunological safety of the intranasal method of revaccination with a live measles vaccine made from the Leningrad-16 strain].

The search for alternative routes for administration of live measles vaccine is associated both with the threat of infection with human immunodeficiency virus, hepatitis B virus, and with the development of a more physiological, natural and less traumatic mode of vaccine administration. The influence of the intranasal administration on the general condition of the immune system, its immunomodulating effect (the emergence of inducer suppressors, cell response to the inactivated virus), was studied as well as the level and intensity of secretory and general humoral immunity. The studies confirmed the immunological effectiveness and safety of the intranasal administration of a live measles vaccine and suggested its advantages for revaccination against measles.

Administration, Intranasal↗
PubMedDate not supplied

[An intranasal method of revaccination against mumps].

The effectiveness of revaccination against mumps was studied using different routes for administration of a live mumps vaccine from L-3 strain. Poor reactogenicity of the vaccine was observed after intranasal administration. The highest rise in titres of virus-neutralizing antibody was demonstrated in volunteers given the vaccine by the intranasal route. Revaccination of adults by both intranasal and subcutaneous routes induced production of antigen-specific memory cells. The results of the study attest to the usefulness of the intranasal route of vaccine administration in revaccinations against epidemic parotitis.

Administration, Intranasal↗
PubMedDate not supplied

[The immunological indices in the intranasal method of revaccination with a live measles vaccine].

Immunological parameters of the blood were studied in intranasal revaccination with a live measles vaccine (LMV) from the strain L-16. Alongside with specific immune response, LMV was shown to produce nonspecific changes in the system of immunocompetent cells the amount and duration of which were vaccine dose-dependent. The immunomodulating activity of LMV administered by different routes was different. Especially marked was the difference in the degree of side effect of the vaccine on T-lymphocytes. A dose of 10,000 TCD50 in 0.5 ml of LMV produced sufficient antigenic stimulation by the intranasal route of revaccination being quite satisfactory from the point of view of immunological safety of measles vaccine.

Administration, Intranasal↗