Biomedical subjects
Publications and source records attributed to V A Molochkov.
The paper contains literature data and the results of author's own observations, devoted to the clinical manifestations, ethiology and pathogenesis of Kaposi's sarcoma. An algorithm of diagnostics of the disease and methods of treatment are offered, which are developed taking into account the type of the disease, its form, the extent of the pathologic process and immunological parameters.
With the aim of forming modern programs for immunogenetic therapy of epithelial tumors, such as methatypic and basal cell carcinomas, the authors of the article analyze the basics of their immunopathogenesis. Having considered the latest immunotherapy regimens, the authors present their clinical assessment.
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The complete ORF54 of the Kaposi's sarcoma-associated herpesvirus (KSHV) (human herpesvirus-8; HHV-8) was cloned and expressed in E. coli. The results show that KSHV/HHV-8 ORF54 encodes a functional dUTPase which specifically hydrolyses dUTP to dUMP. Monoclonal antibodies against the HHV-8 dUTPase detected a protein with the expected molecular mass of 35 kDa in HHV-8-infected BCBL-1 cells. Induction of the lytic replication cycle of HHV-8 by treatment of BCBL-1 cells with the phorbol ester TPA resulted in an increased expression of the protein which was not inhibited by phosphonoacetic acid, indicating that the protein is expressed early in the lytic replication cycle. Moreover, the sporadic expression of the HHV-8 dUTPase in tissue sections of Kaposi's sarcoma was detected by immunohistochemistry.
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There were observed 70 patients (21 men, 49 women) with basaliomas (cancer of the skin). Mental disorders were observed in form of affective disorders (depression, hypothymic colour of the surrounding), syndrome of dismorphophobia-dismorphomania, relatively short cancerophobic feelings, somatogenic asthenia and a development of personality disorders. A forming of the mental disorders were conditioned at basaliomas by a complex influence of both somatogenic and psychogenic factors. An influence of the psychogenic factors was relatively transitory and psychologically comprehending (dysmorphophobia, cancerophobia, depression). An influence of the somatogenic factors predetermined a stability, a duration and a small reversibility of mental disorders (asthenia, irritative weakness, pathological development of the personality).
Fifty one patients with metatypic carcinoma (MC), 10 with basalioma, and 10 with squamous cell carcinoma were clinically and immuno-morphologically studied. There were great differences in the localization of basal cell antigen in the epidermis during MC, basalioma, squamous carcinoma of the skin. Expression of fixed immunoglobulins A, M, G in tumor tissues revealed no significant differences. Fifty one patients underwent a complex treatment with prospidin in a total dose of 3,000-3,500 mg (in a daily dose of 100 mg) followed by near-focus X-ray therapy in a total dose of 3,000 Gy (a single dose being 500 Gy). Good immediate and late (the duration of the follow-up was as long as 5 years) results were obtained.
The results of photochemotherapy of 5 patients with chronic benign familial pemphigus are described. Standard scheme with puvalen and PUVA-22A chamber (Finland) were employed. A course of inpatient treatment consisted of 10-25 irradiation sessions followed by outpatient maintenance photochemotherapy (once a week or two). Erosions and fissures started epithelializing after 8-12 irradiation sessions. A remission of up to 19 months was achieved in all the patients; it was more manifest and durable than after treatment with other schemes.
Standard microlymphocytic cytotoxic test was used in 43 cases of solitary keratoacanthoma. Increased incidence of HLA-B16 and HLA-B18 antigens was established. Presence of HLA-A2 was shown to adversely influence the course of the disease.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Associations of a new human herpesvirus type 8 (HHV-8) with different forms of Kaposi's sarcoma (KS) in Russia have been studied. Search for this virus genetic information has been carried out in biopsy specimens of benign and malignant tumors other than KS, and probable sites of HHV-8 latency in human body have been checked. HHV-8 sequences were detected by polymerase chain reaction (PCR). HHV-8 sequences were most often detected in idiopathic (80.6%), AIDS-associated (80%), and immunosuppressive (100%) KS. The results indicate a selective association of HHV-8 with KS. No probable sites of the virus latency were detected in peripheral blood cells of patients with KS and in the prostate of patients with chronic prostatitis. The only exception was the husband of a patient with KS: HHV-8 sequences were detected in his prostatic secretion by nested PCR.
A case of a rare malignant tumour of the sweat glands is reported. Such tumors are often taken for visceral adenocarcinoma metastasis. The tumor was diagnosed by means of immunohistochemistry.
A case of malignant eccrine cylindroma of the skin studied immunohistochemically is reported.
Vilprafene was used in multiple-modality (immuno-, enzyme, spa, and local therapy) treatment of 15 patients with chronic chlamydial prostatitis. Clinical and etiological cure was attained in all patients without side effects.
Pagetoid reticulosis is a rare form of T-cell skin lymphoma. A case of this disease is reported.