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Biomedical subjects

V A Pavlova

Publications and source records attributed to V A Pavlova.

10 recordsLinked to original sources
PubMed1996

[Variability of satellite DNA II and IV in cattle, various representatives of the subfamily Bovinae and their hybrids].

Polymorphism of satellite DNA II and IV was studied in Bos taurus by means of Southern blotting and dot hybridization. Of primary interest is the absence of restriction fragment length polymorphism at the individual and interbreed levels. Differences in the content of satellite II in the genome are demonstrated for animals of the Kholmogorskaya and Yakutian breeds. The specific features of satellite IV organization in the bison, banteng and yak are revealed, allowing the use of the satellite as a specific genetic marker. Superposition of parental organizational types of this class of repeats is detected for the interspecies hybrids yak x cattle, banteng x cattle, and bison x cattle. At the same time, several cases of deviation from classic inheritance of such parental types in the interspecies hybrids were found.

Animals↗
PubMed1981

[Comparative antibiotic sensitivity characteristics of the staphylococci isolated from ambulatory and hospitalized patients].

Sensitivity of staphylococci isolated from the pathological material of outpatients with purulent inflammatory infections and inpatients was studied with respect to 10 antibiotics. The agar diffusion method with standard paper discs impregnated with the antibiotics was used. It was shown that the majority of the staphylococcal cultures isolated from the outpatients was sensitive to the aminoglycoside antibiotics: neomycin 89.7 per cent, monomycin 87.3 per cent and kanamycin 82.9 per cent. The sensitivity of the isolates to benzylpenicillin, levomycetin, erythromycin and oleandomycin was lower and amounted to 63.5, 64.6, 66 and 69 per cent respectively. The sensitivity of the cultures isolated from the inpatients to benzylpenicillin, erythromycin, oleandomycin and levomycetin amounted to 43.4, 45.5, and 49.5 and 53.3 per cent respectively. The comparative study on the antibiotic resistance pattern showed that more than a half of the cultures isolated from the inpatients were polyresistant (resistance to 4--10 antibiotics). The number of such cultures isolated from the outpatients amounted to 32.8 per cent. Cultures sensitive to all of the antibiotics tested and resistant to 1, 2 or 3 of them predominated in this group of the isolates.

Ambulatory Care↗
PubMedDate not supplied

[The characteristics of chromosomal distribution and of the variability of the multiply repeating DNA fraction of the genome in Bos taurus L].

The uniform distribution of satellite DNA II and IV has been revealed using in situ hybridization and differential staining in centromeric regions of autosomes. The sex chromosomes have not found such nucleotide blocks. There is only minor satellite IV block inside Y chromosome short arm. The Y chromosome has got some (TG)n enriched blocks distributed also among other parts of genome and one copy of sequences like human ZFY gene. The high repetitive fraction of bovine genomic DNA have not revealed RFLP. However, the difference has been found by blot hybridization between genomic organization of satellite IV in cattle and yak chromosomal DNA. Non-Mendelian distribution of some such nucleotide blocks has been obtained for interspecies crosses of cattle and yak.

Animals↗