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Biomedical subjects

V A Sergeev

Publications and source records attributed to V A Sergeev.

At least 19 recordsLinked to original sources
PubMed2000

[The methodological approaches to organizing the rehabilitation of servicemen with amputation defects of the extremities].

10-year experience in prosthetics of extremity stumps in conditions of the Central Military Clinical Hospital N 6 was analyzed. In the hospital during the period from 1989 to 1999 prosthetic appliance was made in 203 patients with amputating defects of extremities, 37 of them with pair stumps and 1 with lack of segments of all extremities. Most patients (80.5%) had stumps at the level of femur and crus. Basing on the analysis of modern scientific literature and experience gained in hospital the authors have formulated organizational and methodical states. Thanks to observance of the stated principles it was possible to optimize considerably the process of medico-psychological and social rehabilitation, to achieve good functional results in all patients treated in the hospital. Adequate use of experience in other military medical and prophylactic institutions will contribute to significant decrease in periods of return of servicemen to social useful labour and increase in efficiency of rehabilitation measures.

Amputation, Traumatic↗
PubMed1999

[Genetic characteristics of the KC vaccine strain of hog cholera virus: comparative analysis of the primary sequence of surface glycoprotein E(rns), E1, and E2 genes].

Primary structure of a genome fragment of attenuated strain CS of hog cholera virus (HCV) coding for three surface glycoproteins Erns, E1, and E2 (fragment size 2379 nucleotides) is analyzed. By the nucleotide sequence the homology between strain CS and ten other virulent and attenuated HCV strains in this area is 84.9-94.6%, 87.2-94.6% in gene Erns, 84.6-96.9% in gene E1, and 83.3-94.3% in gene E2. By amino acid sequence the homology is 90.9-94.3%, 92.9-95.0%, 92.3-95.6%, and 88.9-94.1%, respectively. Computer analysis demonstrated philogenetic ratios between these strains and other HCV strains and the areas of potential antigenic differences between CS strain and other HCV strains. The data indicate that strain CS used as live vaccine protecting from HCV contains unique nucleotide and amino acid positions and its evolution history is different from that of analyzed reference strains. The data will be further used for detecting the fine antigenic structure of strain CS surface glycoproteins with the aim of disclosing unique antigenic markers.

Amino Acid Sequence↗
PubMed1989

Isolation of peplomer glycoprotein E2 of transmissible gastroenteritis virus and application in enzyme-linked immunosorbent assay.

An enzyme-linked immunosorbent assay (ELISA) based on peplomer glycoprotein E2 was developed for the detection of antibodies to transmissible gastroenteritis virus (TGEV). Purified preparations of E2 were isolated by solubilization of the viral membrane with nonion detergent Nonidet P-40, followed by sucrose density gradient sedimentation. ELISA optical density values with E2 antigen significantly exceeded the indices when other TGEV protein or intact virion antigen was used. It was shown that a virus protein concentration in the E2 preparation of 500 ng per well is sufficient to sensitize the solid phase of microplates. A comparison of the ELISA and the virus neutralization test for the detection of TGEV antibodies was conducted. A significant correlation between the ELISA and the virus neutralization test was shown (r = 0.97). This serological test may be successfully used for various immunologic investigations.

Animals↗
PubMed1988

Structure of protein solutions. Part 2. Solutions of total protein of yeast Saccharomyces cerevisiae.

Water systems formed by total protein of yeast Saccharomyces cerevisiae and model systems on the basis of unfractionated casein were studied by viscosimetry and electron microscopy. Methodologically, both methods offered a possibility of distinguishing several quantitatively different concentration intervals in protein solutions under different conditions. Viscosimetry gave the most informative results at the concentration intervals where the solutions approached the true ones, while electron microscopy did so when supermolecular formations prevailed. In comparing the data obtained by these two methods the following conclusions were drawn: At low temperatures (4-10 degrees C) yeast protein was in molecular-dissociated condition. Association in the system starts at 10 degrees C, and becomes enhanced with a rise in temperature. A temperature rise to 25-30 degrees C promotes a more compact packing of polypeptide chains; further heating leads to high-temperature denaturation. Lipids induce covering of polypeptide chains and prevent associations, thus leading to an increase in the system's thermostability.

Caseins↗
PubMed1987

[Rotaviruses: structural and functional organization of the virion].

The data on the structural and functional organization of human rotaviral virion and rotaviral virions of different species of animals obtained by the native and foreign researchers for the last five years of study have been summarized. The virion ultrastructure, the genomic structure and polypeptide composition of rotaviruses are discussed. The data are presented on the transcription and molecular mapping of rotaviral genome. Special attention is paid to localization of rotaviral proteins in the virion capsid, to their function and derivation. The data on the role of endogenous enzymes in rotaviral transcription and replication are presented separately.

Genes, Viral↗
PubMed1986

[Investigation of Soviet microcarriers for cell cultivation].

Home made and foreign preparations (6 patterns in each) were tested as microcarriers for cultivation of anchorage-dependent cells in the medium supplemented with 0.25% enzymatic hydrolysate of muscles and 10-20% animal serum. The best results were obtained with microcarriers DEAE-2.5, Cytodex-1, and DEAE-cellulose. Using home microcarriers DEAE-2.5, continuous swine embryo kidney cells and primary chick embryo cells yielded, respectively, 9-11 and 3-4 times increase.

Animals↗
PubMed1984

[Action of atropine on smooth muscle contractions after administering alpha-adrenoreceptor blockaders].

In acute experiments on anesthetized cats, changes of potentials and motility of the longitudinal and circular layers of the small intestine and stomach were studied in response to atropine administration before and after blocking of alpha--adrenoreceptors (dihydroergotamine, dihydroergotoxin, phentolamine, phenoxybenzamine). The effects of these drugs both on spontaneous and evoked (histamine, i. v., sucrose and NaCl into the gut lumen) motor activity were studied. The inhibitory action of atropine on intestinal motility was partially (in intact animals) or completely (on isolated intestinal segments) abolished after alpha-adrenoreceptor blocking. The mechanism of the atropine effect is discussed in the light of the data on M--cholinoreceptors in the sympathetic postganglionic axons.

Adrenergic alpha-Antagonists↗