Biomedical subjects

V A Shelestin

Publications and source records attributed to V A Shelestin.

2 recordsLinked to original sources

PubMedDate not supplied

[Histone-nonhistone protein complex reconstruction].

A complex of chromatin proteins composed of five histon fractions and nine nonhiston fractions and stable in 2M NaCl solution was destructed by treating with urea, changing pH and decreasing the NaCl concentration, No reconstruction of the complex was observed after the said factors has been removed and the initial solvent composition restored. Nevertheless the reconstruction was detected after dissociation of the complex by desalting in the presence of DNA. The reconstruction was successful when stimulated both dimers and tetramers, the chromatin protein complex under study cannot be reconstructed by "self-organization", the only way being through the stage of nucleoproteid formation, and is similar in this respect to some other complex protein structures of the cell.

Animals↗

PubMedDate not supplied

[Studies of histone-nonhistone complex].

A chromatin protein complex was isolated from the calf thymus using hydroxyapatite without denaturants. Polyacrylamide gel electrophoresis showed that this complex consisted of five histones and nonhistone proteins. The complex is stable in 2 M sodium chloride. As the evidence of its stability the results are presented or rechromatography on hydroxyapatite, of gel-filtration on Sephadex G-150 and of polyacrylamide gel electrophoresis after crosslinking proteins by formaldehyde under mild conditions. It is suggested, that hydrophobic interactions which take place under high ionic strength due to the counteraction of protein charged groups, are among the possible mechanisms. This is supported by partial dissociation of the complex with a decrease in the sodium chloride concentrations in the solution.

Animals↗