V Alemany | PubMed Health

PubMed1992

[Efficacy of auditing in improving a Control Program of Pregnancy].

OBJECTIVE: To compare the results obtained in two successive audits to observe the effectiveness of this control method. DESIGN: The care process followed by pregnant women attended in 1986-87 is compared with that in 1988. LOCATION: Cartuja Health Centre (Granada). PARTICIPANTS: Data referring to 20 markers with defined quality criteria are obtained by auditing clinical protocols. METHODS AND MAIN RESULTS: The qualitative markers were compared to the comparison test of two proportions for separate samples and the quantitative markers with the of student for separate samples and equal variants. The quality index improved in 9 markers and worsened in 1; the remainder did not change. The results prove the effectiveness of quality control to improve care given in a Health Centre.

Female↗

PubMedDate not supplied

New nuclear functions for calmodulin.

The data reported here summarize a series of results which reveal new functions for nuclear calmodulin (CaM). The addition of CaM inhibitors to cultures of proliferating NRK cells blocked the activity of the cyclin-dependent protein kinases 4 (cdk4) and 2 (cdk2), which are enzymes implicated in the progression of G1 and in the onset of DNA replication, respectively. CaM modulates the activity of cdk4 by regulating the nuclear location of both cdk4 and cyclin D, its associated regulatory subunit. By using CaM-affinity chromatography, we have recently identified two new nuclear CaM-binding proteins: (i) the protein La/SSB, which is an autoantigen implicated in several autoimmune diseases such as lupus erythematosus and Sjögren's syndrome (since La/SSB participates in the process of transcription mediated by RNA polymerase III, CaM could be involved in the regulation of this process); and (ii) the protein SAP145, a member of the spliceosome-associated proteins (SAPs) which is a subunit of the splicing factor SF3(b). This finding suggests the involvement of CaM in pre-mRNA splicing. Finally, a screening for new CaM-binding proteins in the fission yeast performed by using the phage display analysis, revealed that several nucleolar-ribosomal proteins associate to CaM, suggesting that CaM modulates ribosomal assembly and/or function.

Animals↗