Biomedical subjects

V B Kuznetsova

Publications and source records attributed to V B Kuznetsova.

3 recordsLinked to original sources

PubMed2006

Spatial organization and morphometric characteristics of histaminergic neurons in the rat brain.

We report here a study addressing the spatial organization, densities, numbers, sizes, and shapes of histaminergic neurons in the rat hypothalamus. Studies were performed on 50 rats using histochemical and morphometric methods, computer image analysis, and three-dimensional reconstruction of the histaminergic nuclei of the hypothalamus. The results demonstrated that the total bilateral volume of the histaminergic nuclei of the rat brain amounts to 0.5 mm(3): the E2 nucleus occupies 40% of the total volume, E4 occupies 35%, E3 occupies 13%, E5 occupies 9%, and E1 occupies 3%. The distribution density of monoamine oxidase B neurons in the histaminergic nuclei of the rat hypothalamus decreased in the order E1 > E3 > E2 (the "compact" nuclei) > E4 (an "intermediate" density nucleus) > E5 (the "diffuse part"). The mean number of histaminergic neurons in the rat hypothalamus was 37200 +/- 2800; the E2 nucleus contained 54% of these cells, E3 contained 23%, E4 contained 6%, E1 contained 7%, and E5 contained 0.32%. The E1-E3 nuclei were dominated by small and intermediate-sized neurons, round in shape, and the E5 nucleus was dominated by intermediate and large neurons, fusiform in shape. A subpopulation of giant histaminergic neurons was detected in the E4-E5 nuclei.

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PubMed2005

[Spatial organization and morphometric characteristic of histaminergic neurons of the rat brain].

The aim of the study was the estimation of the spatial organization, the density of distribution, number, sizes and shape of histaminergic neurons of the rat hypothalamus. The investigation was carried out in 50 adult Wistar rats, using the methods of histochemistry, morphometry, computer image analysis and 3D reconstruction of hypothalamic histaminergic nuclei. It was found that the total bilateral volume of rat brain histaminergic nuclei was equal to 0.5 mm3 : E2 nucleus occupied 40% of their total volume, while E4 occupied 35%, E3 - 13%, E5 - 9% and E1 - 3%. The density of distribution of monoamineoxidase B-positive neurons was decreased in a series E1 > E3 > E2 (compact nuclei) > E4 (intermediate nucleus) > E5 (diffuse part). The average number of histaminergic neurons in rat hypothalamus was equal to (372 +/- 28) x 10(2): E2 nucleus contained 54% of that amount, E3 - 23%, E4 - 16%, E1 - 7%, E5 - 0.32%. In E1-E3 nuclei, small and medium neurons round in shape were prevalent, while in E5 these were medium and large neurons of fusiform shape. The subpopulation of giant histaminergic neurons was found in E4-E5.

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PubMed2003

[The histaminergic brain neuron system].

This review describes the current concepts of distribution, morphological peculiarities and functions of histaminergic structures in human and animal brain. In adult mammals and humans, the perikarya of histaminergic neurons are localized exclusively in the hypothalamus, mainly in its tuberomamillary nuclei, where 5 subgroups are distinguished. It is only there that the syntheses of neuronal histamine takes place and histamine-synthesizing enzyme histidine decarboxylase and corresponding mRNA were found. The processes of histaminergic neurons innervate practically all the brain regions. Histamine action is mediated by three types of receptors: H1, H2 (postsynaptic) and H3 (presynaptic), which are widely distributed in the brain. Histaminergic brain system is involved in the regulation of various functions, systems and reactions of the organism and participates in the pathogeneses of many disturbances and diseases. The review provides the detailed description of the localization of histaminergic neuron perikarya in mammals, the existing classifications of histaminergic neurons of rat brain, the distribution of histaminergic fibers in various brain regions, the organization of human histaminergic brain system, histaminergic neuron ultrastructure, afferent innervation and regulation.

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