[Purification of antibodies for fluorescent diagnosis in influenza].
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Ammonium Sulfate↗
Biomedical subjects
V B Matiushichev
Publications and source records attributed to V B Matiushichev.
[Additional potentialities of using blood cell characteristics for diagnosis of nonspecific ulcerative colitis].
The potentialities of increasing the resolving capacity of standard hematological parameters used for the diagnosis of chronic nonspecific ulcerative colitis were investigated. The mean volume of erythrocytes decreases and the shape of population erythrogram is modified in this disease. White blood is characterized by qualitative changes (mean cell volume and parameters of its distribution for neutrophils lymphocytes, and monocytes). Consideration for the corpuscular volume of these cells helps differentiate between slight, medium, and severe chronic nonspecific ulcerative colitis.
Adolescent↗
[Effect of strophanthin on the electrophoretic mobility of blood erythrocytes in adults and children].
As assessed by changes in the erythrograms asymmetry coefficient, selectivity of the strophantin effect on cell populations with different mobility and age specifics of reciprocal responses, was revealed in adult subjects and in children. The data obtained suggest existence of difference in quality of functional condition of the erythrocytes with normal as well as reduced mobility in children and in adults.
Adult↗
[Dependence of the size of leukocytes on their number in human blood].
An interaction was shown to exist among the quantities of neutrophiles, lympho- and monocytes in circulation and characteristics of their corpuscular volume. The correlation embraces all the leukocyte classes, being apparent in indices of separate classes as well as on the intertype level. Such a co-ordination is involved in maintenance of the normal microcirculation level and manifested in a series of intra- and intertype correlations of relative content and parameters of the corpuscular volume distribution of the cells.
Adolescent↗
[Relations between number and volume of erythrocytes and leukocytes in the human blood].
Total concentration of erythrocytes and leukocytes, a relative contents of neutrophils, lymphocytes, monocytes, average volume of these types of cells, were determined in humans. Changes in the cell indices seem to be correlated and sex-dependent. Thus, erythrocytes and leukocytes are correlated in their contents and volume characteristics. A control mechanism seems to exist in humans, involving microcirculation in capillaries.
Adult↗
[Qualitative difference in blood erythrocytes of men and women].
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Animals↗
[Dynamics of relationship of electrophoretic motility and erythrocyte volume in rats during stress].
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Animals↗
[Electrophoretic mobility and oxidizing status of rat blood erythrocytes at scald trauma].
On model of scald trauma the correlation of electrophoretic mobility of rat blood erythrocytes with intensity of lipid oxidation of erythrocyte membranes and with activity of catalase were studied. It is shown that in dynamics of scald disease development the erythrocyte mobility decreases, the velocity of oxidizing ability of their lipids increases and the protective action of blood antioxidation system is blocked. It is found that there is a close dependence between the level of oxidative processes and electrogenic properties of erythrocytes at the height of manifestation of scald toxaemia. A conclusion was made that the oppressing influence of increasing lipid oxidizing ability a on electrokinetic potential of erythrocytes is of a threshold nature and is realized through change in the membranes' state of these cells.
Animals↗
[High-energy laser therapy of stomach ulcers].
Efficacy of local therapy with copper laser was tried in 105 patients with drug-resistant gastric ulcers. The exposure to yellow-green light each other day brought about scarring of the defects for 15.4 +/- 2.0 days, on the average. In the two-times a week regimen the healing was reached for 24.1 +/- 2.3 days. Separate use of yellow and green light proved highly effective, 42.5% of patients were in remission for 5 years. Frequency of the exacerbation before the treatment and duration of the remission were unrelated. Not a single case of malignant transformation of the ulcer was registered.
Adolescent↗
[Sex differences in the electrokinetic properties of the erythrocytes in normal children and in iron-deficiency anemia].
The electrophoretic mobility of children blood erythrocytes in norm and with iron-deficiency anemia was studied. The age peculiarities and sex specificity of this index was revealed, demonstrated chiefly by dynamics of shape of its distribution curve reflecting a ratio of cell subpopulations with different electrokinetic potential. The state of anemia is accompanied with modification of histogram profiles of both individual and group cell mobility, with patterns of changes also depending on the child sex. The obtained data suggest that at children iron-deficiency anemia the structure or functional status of erythrocyte membrane are changed, but the pathological process does not eventually involve all the populations of circulating erythrocytes.
Aging↗
[The mechanisms of changes in erythrocyte electrophoretic mobility in nephropathology].
A correlation between erythrocyte electrophoretic mobility (EEM) and a complex of clinico-biochemical indices in the blood of patients with chronic pyelo- and glomerulonephritis was studied. It is established that the EEM homeostasis as a whole, comes to the redistribution of cell subpopulation parts with high and low mobility. For keeping stability of EEM, of great significance in the control patients is the total protein content in the plasma, whereas in patients with kidney pathology of primary importance is the level of neutrophils.
Cell Movement↗
[The electrophoretic mobility of the erythrocytes in rats during body adaptation to low-temperature exposure].
The action of a low temperature (-4 degrees C) on electrophoretic mobility of rat blood erythrocytes (EME) was studied. The stress provoked by the low temperature renders a considerable influence on EM of erythrocytes, but the degree and disposition of arising changes depend on the duration of temperature coercion. Two weeks after conducting cooling seances (beginning with 15 min, with an every day increase in exposition by 10 min), the response reaction was manifested in the rise of asymmetry and excess of distribution curve of EME index. The prolongation of cold adaptation to 6 weeks was accompanied with the authentic lowering of the mean mobility of cells, in addition to a subsequent sharp modification of erythrogram profile. The cooling of animals conducted against the background of thyreoidine has given, as a whole, similar results, although the use of the protector alleviated the consequences of low temperature coercion.
Adaptation, Physiological↗
[The paradoxical effect of ethanol on the protein status of a primary chick embryo myoblast culture].
Effects of 0.1-10% ethanol on creatine kinase activity and water-soluble protein amount were studied in extracts of 2 day-old culture of chick embryo myoblasts. Low concentrations of the toxicant stimulated the total creatine kinase activity of the culture whereas middle concentrations exerted no influence, and the highest concentration (10%) oppressed the enzymatic activity. The increase of protein content in extracts was observed when ethanol concentration in the incubation medium was 0.1%. Within the range of 1-5% ethanol the protein content sharply decreased at 10% concentration it did not differs from the control value. The treatment of 5 day-old culture with 1-5% ethanol did not influence either creatine kinase activity or protein content in myoblasts. The obtained data are discussed in the context of chemical hormesis scheme and in terms of unmasked changes involvement in the adaptive reactions of the cell system.
Animals↗
[The relationship of the acid-base status of the blood to the electrophoretic mobility of the erythrocytes in liver pathology].
With cholestatic hepatite, the relationship between acid-alkaline condition of blood and electrophoretic mobility of erythrocytes was studied. It is shown that changes in parameters of the individual mobility distribution are translated into reality independently, and are directed to maintain the steadiness of the index middle level. Dependence of this sign on the pH value, hemoglobin content and bicarbonate-ion concentrating in blood, an on partial pressure of oxygen and carbonic gas was discovered. The intravenous infusion of patients with hemodese restored the middle level of cell electrophoretic mobility previously reduced due to liver pathology. Membrane mechanisms controlling stability of erythrocyte electrokinetic characteristics are discussed.
Acid-Base Equilibrium↗
[The control mechanisms of the electrokinetic properties of human erythrocytes in emotional stress].
A study was made of the influence of psycho-emotional strain, experienced by students when taking examinations, on the erythrocyte electrophoretic mobility (EPM). It has been shown that students under psycho-emotional strain the mean value of EPM remains the same, but changes were observed in the form of EPM distribution, while estimated by asymmetry and excession coefficients. Similar results were obtained when adrenalin was added to the blood.
Educational Measurement↗
[The creatine kinase activity of chick myoblasts and neuroblasts in primary culture after intensive laser irradiation].
The influence of green-yellow light of copper laser (mean power of impulse-periodic emanation 3 W, working density of power in experiment is 200 mW/cm2) on biochemical indices of chick embryo myoblasts and neuroblasts in primary culture was studied. The content of water extracted protein was found to increase progressively, the total and specific activities of creatine kinase elevated considerably in both the cell cultures under irradiation with the total doses equal to 3 and 15 J. However, with dose of 30 J these parameters corresponded to the level characteristic of nonirradiated cultures. Mechanisms of stimulating action of highly intensive laser emanation on cell functions are discussed.
Animals↗
[Changes in the cell membrane permeability of chick myoblasts in primary culture when in contact with rat neutrophils].
The reaction of chicken myoblasts in competent primary culture during their contact with activated rat neutrophils was evaluated. In the incubation medium dynamics of activities were defined for proteinases and creatine kinase, protein content, as well as for DNA and protein marked precursor increase. An increase in myoblast membrane permeability has been revealed. It is shown that the culture is a sensitive model for investigation of physiological interaction of neutrophils with muscle tissue cells.
Animals↗
[Modification of biochemical changes in developing cultures of chick embryo nerve tissue cultures].
The effects of exogenous ganglioside GM1 (1 microM) from bovine brain on the morphological state and biochemical parameters (creatine kinase, acetylcholinesterase and adenylate cyclase activities as well as the protein, phospholipid and ganglioside content) have been studied in primary cultures of trypsin-treated dissociated cells of chicken embryonic brain. Ganglioside GM1 accelerated the growth and differentiation of cultured cells, increased the phospho- and glycolipid content and stimulated the activity of the enzymes.
Animals↗