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V Bergroth

Publications and source records attributed to V Bergroth.

58 records · Page 4Linked to original sources
PubMed1981

Skin lesions in Waldenström's macroglobulinaemia. Characterization of the cellular infiltrate.

A patient with Waldenström's macroglobulinaemia presenting with numerous red or brownish-red, round, papulous lesions of the skin is reported. Skin biopsy revealed a massive infiltrate of immature lymphatic cells of the dermis down to the subcutaneous fat. Approximately 80% of these cells were positive for intracellular IgM and kappa light chains as shown by the immunoperoxidase method. This finding established the skin lesions as part of Waldenström's macroglobulinaemia.

Aged↗
PubMed1980

Sensitivity and nonspeicific staining of various immunoperoxidase techniques.

Optimally fixed paraffin enbedded tissue sections and cytocentrifuged cell smears were used to test the sensitivity and nonspecific staining with the enzyme-bridge, PAP, indirect and direct immunoperoxidase methods using human immunoglobulins and lysozyme as antigens. With the enzyme-bridge method positive staining was seen with primary antiserum dilutions up to 1:20,000. The least background staining was observed with this method. The PAP method was equally sensitive, although false-negative results with low primary antiserum dilutions were seen. Some nonspecific background staining always persisted using the PAP method even with high primary antiserum dilutions. The indirect method was not as sensitive as the enzyme-bridge method and some nonspecific staining always persisted. The direct method was too insensitive with paraffin embedded tissue sections.

Animals↗
PubMedDate not supplied

Immunohistochemical identification of inflammatory cells in secretory and chronic otitis media and cholesteatoma using monoclonal antibodies.

Monoclonal antibodies (OKT 3,4,6,8 and OKIa) were used in conjunction with the avidin-biotin-peroxidase complex method to classify inflammatory cells in 6 biopsies of the middle ear mucosa in patients with secretory otitis media (SOM) and in 19 middle ear or mastoidal biopsies in patients with chronic otitis media (COM). Mononuclear cell infiltrates under the mucosal epithelium were found to consist mainly of T4 positive (helper-inducer) T-lymphocytes (50-60%). T8 positive (suppressor-cytotoxic) T-lymphocytes accounted for 20-30% of the cells. T4 positive cells were confined to the round cell infiltrates, whereas T8 positive cells were also located under the mucosal epithelium and seemed to penetrate it. There were no differences between SOM or COM with regard to the distribution or localization of T4 and T8 cells. T6 and Ia positive Langerhans cells were found in the ingrowing tympanic membrane squamous epithelium and in the cavity skin. The number and distribution of these T6 positive cells were similar to those observed in the skin. In the thick cholesteatoma epithelium these cells were somewhat unevenly distributed but were more numerous than in other sites studied. The results are indicative of a normal cell-mediated mucosal response to infection.

Antibodies, Monoclonal↗