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Biomedical subjects

V Bocci

Publications and source records attributed to V Bocci.

15 recordsLinked to original sources
PubMed1979

Studies of factors regulating the ageing of human erythrocytes. II. Metabolic depletion of erythrocytes is not accompanied by a decrease of their sialic acid content during blood bank storage.

Erythrocytes from human blood stored at 5 degrees C in anticoagulant-preservative solutions are considered, after a period from 21 to 24 days, unsuitable for transfusion. The nonviability of the cells appears due to metabolic impairment accompanied by a change of the biophysical properties of the cell but surprisingly not to a decrease of sialic acid of the membrane. The absence of proteolysis can be attributed to calcium chelation and to the stable levels of plasma protease inhibitors throughout the storage period. In fact, when erythrocytes are incubated for several days at 5 degrees C in protein-free media, release of sialopeptides from the membrane increases slowly and steadily, particularly if the medium is without glucose and adenine but contains divalent cations. Moreover, this phenomenon is strikingly amplified when incubation in protein-free media is carried out at 37 degrees C and once the ATP level has fallen below 5% of its initial value.

Adenine↗
PubMed1977

The role of sialic acid in determining the survival of circulating interferon.

Rabbit interferon has been extensively desialytated and its metabolic behaviour has been evaluated in the rabbit. The half-life of asialointerferon is significantly shorter than the native interferon and its urinary excretion becomes negligible. Moreover the rapid uptake of asialointerferon by the isolated and perfused rabbit liver, suggests a hitherto unsuspected catobolic pathway for this glycoprotein.

Animals↗
PubMed1977

Metabolism of interferon: hepatic clearance of native and desialylated interferon.

The effect of removal of sialic acid on the survival of rabbit serum and urinary interferon (IF) has been investigated in isolate, perfused rabbit liver preparations. In contrast with native IF, which may be already partially desialylated. IF freed of 80 to 90% of its sialic acid was rapidly cleared from the perfusate of normal livers, or livers pre-treated with actinomycin D. The results suggest that the mechanism of IF catabolism by the liver is similar to that reported for several other circulating glycoproteins.

Animals↗
PubMedDate not supplied

Metabolism of interferon (facts and fancy).

Although the knowledge of the interferon metabolism is still incomplete, two organs are emerging as elective sites. The kidney and probably the liver may share a major role but only future research will define quantitatively their importance. Many aspects of interferon activity upon cellular processes and its possible function as a 'chalone-type' substance are reviewed.

Animals↗