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Biomedical subjects

V Bouropoulou

Publications and source records attributed to V Bouropoulou.

16 recordsLinked to original sources
PubMed1997

Computerized determination of proliferating cell nuclear antigen expression in meningiomas. A comparison with non-automated method.

Proliferating cell nuclear antigen (PCNA) expression has been proven to be a significant marker of cell proliferation in meningiomas, which correlates with growth rate and, as shown by several authors, possibly provides prognostic information concerning biologic behavior. However, the current method for determining PCNA labeling index (LI) is tedious and time consuming like all the nonautomated methods for evaluating cell kinetics, presenting high interobserver and interlaboratory variability and low reproducibility. In the present study, we introduce a semi-automated computer-assisted image analysis method for determining PCNA LI in 38 meningiomas, in parallel with the current nonautomated method. Image analysis technique permits unbiased cell counting, standardizes the degree of staining intensity and provides instant results. By calculating coefficient of variability, the method proved to be highly reproducible. The correlation between the results provided by the nonautomated and the semiautomated image analysis method showed a high agreement between them, with a correlation coefficient, r, of 0.82. In conclusion, we consider that image analysis contributes to the accuracy, reproducibility, and practicality of PCNA LI determination so that along with other useful parameters this significant marker may serve to predict the clinical behavior in meningiomas.

Histological Techniques↗
PubMed1991

Ag-NORs in giant cell tumor of bones: are they useful in the estimation of tumor's behavior?

The number of nucleolar organizer regions (NORs) in giant cell tumors of bones was measured using the one-step silver staining technique (AgNOR) with black and white photographs and semiautomatic image analysis. The purpose of the study was to determine whether this method provides useful information on the biological behavior of bone GCTs. The number of AgNORS in bone CGTs (mononuclear cells: 3.45 +/- 1.17, and multinucleate cells: 2.52 +/- 0.97) was found to be higher than in NOF cells (2.16 +/- 1.02 and 2.01 +/- 0.78) but lower than in MFH cells (4.17 +/- 1.3 and 3.86 +/- 1.24). Statistical analysis showed that differences between GCTs and MFH were highly significant for both mononuclear and multinucleate cells, whereas differences between GCTs and NOF were significant only for mononuclear cells.

Bone Neoplasms↗
PubMed1990

Primary vascular bone sarcomas. A clinicopathological and immunohistochemical study of two cases.

Two cases of primary vascular bone sarcomas are reported. The first case presented a rather slow clinical course and a non-aggressive histological picture and was classified as hemangioendothelioma. The second was a frankly malignant tumor, already metastatic at the time of diagnosis, which was classified as angiosarcoma. Views concerning the nomenclature and the behavior of primary vascular bone sarcomas are also discussed.

Aged↗
PubMed1990

Follicular thyroid adenoma: signet ring cell variant.

An unusual case of a microfollicular adenoma of the thyroid gland with signet-ring cells in a 36-year-old euthyroid man is described. Mucin stains demonstrated the presence of mainly neutral glycoproteins with focal participation of acidic groups. Immunohistochemical staining for thyroglobulin was strongly positive. The apparent "mucin secretion" described in these rare neoplasms could be due to the histochemical staining of carbohydrate components or breakdown products of thyroglobulin and colloid. The published work on primary mucin secreting tumors of the thyroid gland is reviewed.

Adenoma↗
PubMed1990

Echinococcosis of bone: clinico-laboratory findings and differential diagnostic problems.

Twenty cases of echinococcosis affecting the skeleton are reported. These cases were diagnosed at the Pathology Department of KAT General Hospital over a 12 year period (1976-1988). The parasitosis involved individual bones or skeletal parts (spine 6, pelvis 6, femur 4, tibia 1, scapula 2 and maxilla 1). The age of the patients ranged from 20-74 years (mean age: 52 years) with preponderance of men. The diagnostic and differential diagnostic problems as well as the therapy and prognosis of this infestation are discussed.

Adult↗
PubMed1989

Immunohistochemical investigation of chordomas: histogenetic and differential diagnostic aspects.

Chordomas are rare tumors of neuroectodermal origin and often show a very heterogeneous histological picture. In a combined histochemical and immunohistochemical study of 32 chordomas collected in the Bone Tumor Registry of Westphalia we were able to show that the immunoreactivity of the cells in both chordoma and notochordal structures are in close relationship with the extracellular matrix and depends more on the metabolic activity of these cells than on the origin of the cells of the neuroectoderm. All tumor cells show a bimodal immunoreaction with cytokeratin and vimentin, as well as a strong immunoreaction with the oncofetal markers CEA and AFP. The differentiation of chordomas from other malignant tumors, mainly the myxoid variant of chondrosarcoma, may cause major difficulties, especially if only a little biopsy material is available. Here we can see that in tumors with bimodal immunoexpression of vimentin and cytokeratin, as can be found in chordomas, the further use of antibodies offers a reliable differential diagnostic tool. The positive reaction of chordomas with all epithelial tumor markers offers a clear differentiation from chondrosarcomas, which, unlike chordomas, do not express cytokeratin. The identification of a marker profile by employing common antisera is of major value in the differentiation of chordoma from other epithelial or mesenchymal tumors.

Adult↗
PubMed1986

Tubal conjoined twin pregnancy.

A unique case of tubal conjoined twin pregnancy is reported. The embryos presented a lateral type of conjunction, which also is extremely rare, and were classified as symmetrical iliothroacopagus conjoined twins.

Adult↗
PubMed1985

A histological and immunoenzymatic study on the histogenesis of "giant cell tumor of bones".

We carried out a prospective histologic and immunoenzymatic study, using lysozyme and AI-antichymotrypsin, of 15 well documented cases of giant cell tumors of bones. The histologic appearance of the majority of the tumors was characterized by great pleomorphism. The predominant histologic patterns could be classified as either fibroblastic or histiocytic. Mitoses were seen exclusively on stromal mononuclear cells. All tumors showed positive marking with both lysozyme and AI-antichymotrypsin. The enzymatic activity was more pronounced in areas of conventional histology and appeared as coarse orange-brown granules in the cytoplasm of many mononuclear and multinucleated giant cells. Enzyme-positive cells were less frequently found in fibroblastic areas of the tumor and especially in areas with minimal differentiation. The results indicate that giant cell tumor of bones may result from the neoplastic proliferation of mononuclear cells which in many areas of the tumor differentiate to either fibroblasts or histiocytes. Thus, giant cell tumor of bones may be analogous to fibrohistiocytic tumors of soft tissues.

Adult↗
PubMed1984

Microcalcifications in breast carcinomas. A histological and histochemical study.

Microcalcification in breast carcinomas were studied histologically and histochemically in 332 prospectively collected mastectomy consecutive specimens, without previous roentgenographic examination. Microcalcifications were found in 41% of breast carcinomas. The incidence was higher in comedo-carcinomas, duct carcinomas nonotherwise specified (N. O. S.) and papillary carcinomas. In situ and infiltrative lobular carcinomas did not show any microcalcification. Histochemically, calcium was shown to be deposited in compound lipids. The morphology and localization of microcalcifications was similar to those described in fibrocystic disease. This should be ascribed to similar structural and cellular conditions in both diseases.

Breast Neoplasms↗
PubMed1984

Microcalcifications in benign breast diseases. A histological and histochemical study.

A hundred cases of fibroadenomas and 300 cases of benign breast diseases were studied without previous roentgenographic examination of the specimens. Microcalcifications were found in 19% of the fibroadenomas, in 26% of the cases of "fibrous disease" and in 57% of the cases of fibrocystic disease. Benign microcalcifications were not found in normal mammary tissue. They were steadily associated with metaplastic epithelial cells and with their abnormal secretions. Two basic types of microcalcifications were encountered: The granular microcalcifications with a predominately intracellular location and the homogeneous (with or without concentric rings) mainly associated with secreted materials. Microcalcifications were associated with metaplastic epithelial cells of the small eosinophilic variety and with their secretions, with the foam cells and with materials secreted by the apocrine cells. A common histochemical feature in cells or matrixes containing microcalcifications was the presence of compound lipids. The possible relationship of benign breast microcalcifications to the calcifications of other tissues, in connection with their association with lipids is discussed.

Adenofibroma↗
PubMed1983

Mucous metaplasia of epithelial cells in fibrocystic disease of the breast. A histochemical study.

Mucous metaplasia of breast epithelium was studied by histochemical procedures in cases of fibrocystic disease. Epithelial mucins consist almost entirely of non-sulfated sialomucins. A slight amount of sulfated sialomucins was identified occasionally while neutral mucins seems to be absent. Abundant glycogen granules are contained in the cytoplasm of metaplastic cells. The secretion of sialomucins may be associated with a loss of cell antigenicity. In this case, the survival of metaplastic epithelium supports the hypothesis of the possible immunological nature of the disease.

Epithelium↗
PubMed1979

Duct elastosis in infiltrating carcinoma of the breast.

Duct elastosis was studied in 219 patients subjected to radical mastectomy for infiltrating carcinoma of the breast, with a 10-year follow-up. Duct elastosis is a frequent finding in infiltrating breast cancer (65% of our cases). It develops in tumors of all three grades of malignancy, but it is more frequent in tumors of low grade malignancy (76% and 74% in grades I and II, respectively, and 47% in grade III tumors). In spite of their greater incidence in low malignancy tumors, the elastotic cases have a greater metastatic ratio than the non-elastotic cases (66% vs 45%). The elastotic cases also contain a significantly greater proportion of scirrhous tumors than the non-elastotic cases (86% vs. 32%). Duct elastosis and scirrhous reaction are two processes which develop in parallel, but are not related etiologically. They seem to be correlated with more advanced stages of the neoplastic disease. The influence of duct elastosis upon the ten year survival of the patients is unfavorable. this influence is not direct, and it is particularly evident in the metastatic cases. It seems to be related to the greater duration of the neoplastic disease and to the slow clinical course of tumors of low degree of malignancy.

Breast Neoplasms↗