Biomedical subjects
Publications and source records attributed to V D Popov.
Experimental estimation of properties of an activated carbonic fibrillar material "Dnipro" was conducted. Its application perspectivity while conduction of plasmo-, lympho- and hemosorption was proved.
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A universal soft-art complex tentatively named Arm-Stomatolog, covering all sections of dentistry, has been developed. It is based on individual programs in dentistry, making use of high-level algorithmic languages fitted to modern operation systems and permitting block-to-block fitting of individual programs to unite them in a complex.
A specific method of the isolation of the cholera toxin gene by the directional amplification of DNA in the polymerase chain reaction (PCR) has been developed. The product of this reaction has a molecular weight of 440 sequence pairs and is a DNA fragment located on the A-subunit of V. cholerae gene vct. The sensitivity of the method permits the detection of one bacterial cell in the reaction mixture. The method is effective when V. cholerae purified DNA, cell lysates and the DNA of total microflora isolated from the water of natural springs are used. The study of water samples from natural water bodies by the method of PRC has revealed cholera toxin genes of V. cholerae noncultivated forms ni 5 out of 7 water samples taken from natural water bodies at the regions of Azerbaijan endemic for cholera and made it possible to evaluate the number of V. cholerae. The prospects of using PCR for the control of the epidemiological situation in regions endemic for cholera are discussed.
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Investigated were the absorption spectral curves of alkaline protein hydrolysates and DNA-extracts from bull, ram and boar spermatozoa employing the method of Schmidt-Tannhauser. It was established that the double-wave method could be used to determine the content of DNA in the investigated ejaculated at the following values of the two waves and coefficients: 268 nm and 280 nm; K = 1350 for ram spermatozoa, and 268 nm and 281 nm; K = 1175 for bull and boar spermatozoa. The evaluation of the quantitative content of DNA per spermatozoid of bull, ram and boar was also carried out, establishing that the figures obtained are approximately equal.
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