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Biomedical subjects

V E Komar

Publications and source records attributed to V E Komar.

46 records · Page 3Linked to original sources
PubMedDate not supplied

[Endonuclease activity in the blood serum of rats undergoing nonuniform gamma irradiation].

During the first hours following nonuniform gamma-irradiation (the nonuniformity coefficient was 3:1 and 5:1 with a dose change along the axis directed cranio-caudally and caudo-cranially), endonuclease activity (pH 5.75) in the blood serum increased with the dose which was estimated with a reference to total utilization of the substrate. Supercoiled plasmid DNA served as the substrate. The transformation of a supercoiled form of plasmid DNA into an open circular form was the criterion of estimation of nuclease activity. It was shown that the increase in the nuclease activity correlated with the increase in the nonuniformity coefficient.

Animals↗
PubMedDate not supplied

[Change in the metabolic reserve of free amino acids in the peripheral blood and spleen during exposure to whole body uniform gamma-radiation].

A study was made of the dynamics of changes in the amino acid composition of erythrocytes, plasma, whole blood and spleen of albino rats during 3 days after total-body uniform gamma-irradiation with doses of 3, 6 and 12 Gy. The change in the amino acid pool of blood and spleen and the ratio between the amino acid concentration in erythrocytes and plasma were shown to depend upon the degree of radiation injury.

Amino Acids↗
PubMedDate not supplied

[Effect of novobiocin on degradation and repair of the superhelical structure of nuclear DNA from rat thymocyte fractions].

A study was made of the action of novobiocin on degradation and repair events in supercoiled nuclear DNA from three thymocyte fractions obtained by ficoll-paque gradient sedimentation. When added before gamma-irradiation novobiocin (1.9 mg/ml) exerted a radioprotective effect during the "second wave" of supercoiled DNA degradation. It is suggested that this effect may be due to the inhibition of DNA topoisomerase II.

Animals↗
PubMedDate not supplied

[Radiation damages in human lymphocytes studied by micronucleus and chromosomal analysis].

It was shown that the dependence of the micronucleus number and chromosome aberration on irradiation dose is linear quadratic, the values of linear coefficient being different. The distribution of ameliorators and children from the areas with higher irradiation level by micronucleus test conforms to Poisson distribution, while the distribution by chromosomal analysis approaches to binomial distribution.

Adult↗
PubMedDate not supplied

[A cytogenetic study of patients with disordered thyroid homeostasis].

The cytogenetic disturbances in peripheral blood lymphocytes from patients with various thyroid diseases were studied. It was found that hormone administration results in chromosome aberration and micronucleus (MN) rate increase. The changes in the chromosome aberration spectrum correspond with radiation induced changes in the spectrum. The additional irradiation of cells in vitro caused the lower level of MN in patients treated with hormones than in control donors group.

Blood Donors↗
PubMedDate not supplied

[A decrease in the damaging action of gamma irradiation on human lymphocytes by postradiation treatment with the preparation MDI].

The effect of MDI, the agent of plant origin, on the cell cycle and the number of micronuclei in human lymphocytes after gamma-irradiation has been studied. It has been found that the treatment of lymphocytes with MDI stimulates DNA synthesis and reduces the delay of irradiated cells in (G2 + M) phase. Moreover post-irradiation cell treatment with MDI reduces the number of damaged cells.

Cells, Cultured↗