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Biomedical subjects

V E Pigarevskiĭ

Publications and source records attributed to V E Pigarevskiĭ.

At least 19 recordsLinked to original sources
PubMed1992

[A hypothesis about resorptive cellular resistance as a special form of antimicrobial host protection].

The ability of macrophages to phagocyte granulocyte debris ("cleaning" reaction) is evolutionary developed and represents the mechanism of cellular resistance preceding immunity. Activation of the mononuclear phagocyte system and acquisition by the macrophages of the capacity to the intracellular chlamydia destruction as well as other infectious agents parasitizing in the intact cells occurs in the process of the granulocyte debris phagocytosis. This phenomenon termed by the author as a resorptive cellular resistance is one of host barrier mechanisms.

Animals↗
PubMed1990

[Age-related immunodeficiencies of the neutrophilic granulocyte system].

Transitory age-associated deficiency of the granulocyte cationic proteins as a cause of a phagocytosis disturbance, loss of the phagocyte cell ability to kill phagocytosed bacteria is established. Quantitative indexes of myeloperoxidase content, elastase and catepsin G in the neutrophil granulocytes of newborns and laboratory animals are determined. Hypothesis is developed on the transitory age-associated immunodeficiencies of the neutrophil granulocyte system as one of the endogenic risk factors in the development of infectious diseases during early postnatal period.

Aging↗
PubMed1990

[The molecular level of general pathological processes].

The knowledge of the molecular level of the general pathologic processes is a prerequisite for new concepts realized in the practice. The molecular destructive processes, programmed cell death and inflammation are considered as examples. The hypothesis of the catastrophe of errors is reviewed, the results of studies on the limited proteolysis reaction as well as intracellular destructive processes compatible with the life of cell are summarized. The concept of nonphagocytic tissue resistance and resorptive cellular resistance as a peculiar type of nonspecific protective reactions in the infectious inflammation is substantiated.

Cell Survival↗
PubMed1989

[The role of polymorphonuclear leukocytes in the mechanisms of ulcerogenesis].

The functional activity displayed by polymorphonuclear leukocytes (PL) of the peripheral blood and gastric mucosa, including ulcer margins, was studied in 50 rabbits with ulcer experimentally induced by the Okabe method and in 25 intact animals. The peripheral blood PLs were found to show substantially increased lysosomal cation proteins (LCP), enhanced alkaline phosphatase and decreased peroxidase activity. The changes in the gastric mucosa, particularly in its damaged area, were characterized by a marked microcirculatory bed response and formation of lymphocytic and, later on, pronounced neutrophilic infiltrates. The tissue PL therewith contained abundance of glycogen and LCP and intensively secreted granules. The maximum of these manifestations as viewed from PL coincided with the ulcer formation period (on days 2-3 of the experiment). When the ulcer had developed, the levels of LCP and glycogen in PL became lower. It was concluded that PL made an important contribution to ulcerogenesis.

Acetates↗
PubMed1989

[A method for evaluating a diagnostic inflammatory focus by using the "cold test"].

Clinico-morphological test for the quantitative evaluation of exudative and cell response in the diagnostical inflammatory focus in various forms of human pathology is developed. The values in practically healthy persons are established. The possibilities of the test use for the prognosis of disease and the development of postoperative complications in patients with cold trauma are shown.

Adult↗
PubMed1988

[Effect of UV radiation and the autotransfusion of UV-irradiated blood on the content of cationic proteins in the neutrophilic granulocytes of calves].

A study was made of the influence of UV-irradiation (254 nm) of blood in vitro, of the autotransfusion of UV-irradiated blood (AUVIB), and of the mixture of UV-irradiated and intact blood in vitro on the content of bactericidal cation proteins (CP) in blood neutrophil of calves suffered from dyspepsia and broncho-pneumonia. Age differences were noticed in CP contents and their decrease in neutrophils following AUVIB in vivo and administration of the mixture of blood in vitro. The decrease in cell CP contents is presumably due to neutrophil degranulation and CP release into the blood plasma. Since the initial mechanisms of neutrophil degranulation are located on the cell surface, the CP release is supposed to result from a membranotropic effect of UV-irradiated blood on the intact autologous blood. This effect may explain the increase in nonspecific resistance of organism after the AUVIB, being one of the main therapeutic phenomena of the AUVIB-therapy.

Animals↗
PubMed1988

[A method for assessing the lysosomal secretory activity of neutrophilic granulocytes using a diagnostic focus of inflammation].

Lysosomal secretion of neutrophilic granulocytes has been studied in normal subjects (healthy males and females aged 20-50), pregnant women and burnt patients. A significant rise in lysosomal secretory activity of neutrophilic granulocytes was recorded in the 3rd trimester of pregnancy. Low lysosomal secretion can serve an indicator of poor prognosis in patients with a severe burn trauma.

Adult↗
PubMed1988

[Degree of structural homology of lactoferrins from milk and neutrophilic granulocytes].

Some physico-chemical properties of human and pig lactoferrins from milk and neutrophilic granulocytes were compared. It was shown that the lactoferrins from different cell and tissue sources of the same species (humans or pigs) are identical in terms of electrophoretic mobility, molecular weight, iron-binding capacity, absorbance spectra, amino acid and sugar compositions and peptide maps. Human and pig lactoferrins show a high degree of structural homology (approximately 50%), but are immunochemically different.

Amino Acids↗
PubMed1987

[Express evaluation of the severity of the course of lactation mastitis].

The authors consider that quantitative and qualitative characteristics of phagocytosis can be given with the help of lysosomal-cationic test (LCT) at different periods of acute purulent lactation mastitis. LCT gives prognosis of the course of the disease and a reliable estimation of the treatment efficiency.

Blood Bactericidal Activity↗
PubMed1987

[Clinico-morphological tests to assess the function of neutrophilic granulocytes].

Clinico-morphological tests for the estimation of neutrophil granulocyte function were compared by their reproducibility, reliability and availability for standard clinical laboratories, and the results were analysed. Lysosomal-cationic test was offered for clinical practice, as it gives qualitative and quantitative assessment of the phagocytosis process, allows prediction of complications before their clinical onset, performance of rapid evaluation of the therapy used, reveals shifts in the level of non-specific resistance of the body. Methods of application of lysosomal-cationic test in the pathoanatomic practice have been developed for the study of intravital biopsy samples and surgical material.

Cations↗
PubMed1986

[Method of staining for lysosomal cationic proteins in histological paraffin sections of biopsies].

A method is suggested for a histochemical and immunofluorescent detection of lysosome cation proteins in the histologic paraffin sections of biopsies. The method is based on the staining of histologic sections obtained from the material fixed in glutaraldehyde buffered with an alcohol solution of a fast green (pH 8.1-8.2) and on the detection myeloperoxidase and lactoferrine by means immune serum by Coons's method. 0.25% solution of azur A (B, C) is used for staining the cell nuclei and viable bacteria.

Biopsy↗
PubMed1986

[Detection of cationic proteins and peroxidase in semithin sections].

A method of histochemical identification of cationic proteins, peroxidase, viable and killed bacteria in semithin sections of biopsy samples is suggested. The method is based on dying of araldite-embedded semithin sections with a buffered alcohol solution of fast green (pH 8.15) and on peroxidase identification with O-dianisidine. The method suggested can be used for distinguishing occult forms of infectious gastritis, evaluation of secretory and barrier function of gastric epithelium, selective dying of leucocytes in tissues, revealing of viable and non-viable bacteria in the infective foci.

Adult↗
PubMed1985

[Isolation and physico-chemical properties of lactoferrin from swine neutrophils].

Lactoferrin, a non-heme iron-binding protein was isolated from pig neutrophils. The purification procedure included initial extraction of the protein in the presence of cetyltrimethylammonium bromide followed by chromatography on carboxymethyl-cellulose and Sephadex G-100. The thus obtained protein was found to be homogeneous on polyacrylamide gel (PAAG) electrophoresis at acidic values of pH. PAAG electrophoresis in the presence of sodium dodecyl sulfate revealed a single component with a molecular weight of 75 000-80 000. The resulting protein is capable of binding two atoms of iron molecule. The absorbance spectra for the pig neutrophil lactoferrin are identical to those for cow milk lactoferrin in the visible region and have a maximum at 465 nm. The amino acid composition of pig lactoferrin was determined. Isoelectric focusing of the protein obtained in a PAAG stabilized pH gradient revealed a component with pI of about 6.8. A single precipitin line was observed with rabbit antipig lactoferrin when examined by immunodiffusion. No immunological cross-reactions were observed between pig lactoferrin and bovine lactoferrin.

Amino Acids↗