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V G Bogush

Publications and source records attributed to V G Bogush.

At least 19 recordsLinked to original sources
PubMed1985

[Modular structure of the genes of phages-transposons of Pseudomonas aeruginosa].

The basic criterion to confirm the recombinational origin of bacteriophages belonging to the same phage family is revealing several different combinations of differentiated segments in phage genomes which determine specific functions (modules). The results of phage-to-phage comparison of several regions in genomes of closely related transposable phages of Pseudomonas aeruginosa D3112, B39, PH2, PH51, PH93, PH132 have supported the modular hypothesis for this group of phages.

Attachment Sites, Microbiological↗
PubMed1984

[Wide distribution of transposable phages in natural Pseudomonas aeruginosa populations].

Five phages (PH2, PH51, PH59, PH93 and PH132) which have some characteristics common with D3112, the transposable phage of Pseudomonas aeruginosa, were isolated from clinical P. aeruginosa isolates. The phages were distributed into 4 different immunity groups. The basic criteria used for selection of transposable phages have been: 1) Morphology of a phage particle, host range, similar inactivation with antiserum; 2) Similar sizes of phage genomes; 3) The presence of a variable non-phage nucleotide sequences covalently linked to phage genome DNA, which could be identified using restriction endonucleases or by heteroduplex analyses. The DNAs of the new phages are resistant to treatment with BamH1 endonuclease, like the DNAs of phages D3112, B39 and B3 described earlier. The restriction maps of the phage genomes are constructed.

Bacteriophages↗
PubMed1983

[Use of deletion mutants of plasmid RP4::D3112 for the genetic analysis of Pseudomonas aeruginosa bacteriophage D3112].

The hybrid plasmid RP4::D3112 becomes unstable in Escherichia coli K-12 cells under certain growth conditions. The deletion mutants of this plasmid are formed at a high frequency. All the deletions selected have a specific feature: they start in the left end, at the point of joining of plasmid and phage DNA, and remove different portions of the phage genome. The deletion mutants have been used for genetic mapping of D3112. We have localized the repressor gene cI (0-1.3 kb), 3 early genes (1.3-14.2 kb) and two groups of late genes (14.2-29.9 and 29.9-38 kb). Electron microscope studies of RP4::D3112 DNA and its deletion derivatives have shown that integration of D3112 genome in RP4 occurs through the ends of the genome, without permutations. It appears that bacterial nucleotide sequences joined to DNA from mature D3112 particles, to the right end of D3112 genome, are lost. Thus, transposable phages D3112 of Pseudomonas aeruginosa and E. coli Mu phage have some similarities in the genome organization and in the way of their integration into the host DNA.

Bacteriophages↗
PubMed1982

[Interaction of Pseudomonas aeruginosa plasmids and mu-like phages: the suppression of the early stages of cell infection by phage D3112 in the presence of plasmid RPL11].

The growth of Mu-like, D3112, B39 and B3 bacteriophages of Pseudomonas aeruginosa on bacterial strains containing R plasmids was studied. Plasmids RPL11, Rms148 and Rms163 were shown to interfere with phage growth: 1) D3112 and B39 phages do not produce plaques on a lawn of PAO1 (Rms148) giving e.o.p. less than 10(-9); 2) RPL11 plasmid restricts phage D3112 growth (e.o.p. less than 10(-9), the growth of phage B3 being also restricted by this plasmid, though in considerably less extent; 3) phage B39 makes small and very turbid plaques on a lawn of PAO1 (Rms163) with e.o.p. 0,13, while c mutants form clear plaques on this lawn and grow with e.o.p. 1,0. The interference of plasmid RPL11 with phage D3112 growth was examined in detail. The plasmid did not affect phage D3112 adsorption and no restriction of phage DNA in R+ cells was found. However, phage genes controlling establishment of lysogeny and the lytic cycle were not expressed after infection. It was observed though, that if a cell contains both prophage D3112 and plasmid RPL11, no interference with repressor synthesis or phage development takes place after induction of prophage. The results obtained allow to conclude that: 1) RPL11 plasmid interference with phage D3112 growth is caused by the plasmid effect on one of the early stages in the development preceding phage DNA integration; 2) the process of primary integration after infection and that of reintegration of DNA after prophage induction are likely to differ.

Adsorption↗
PubMed1981

[Artificial Tn2551 transposon with replicative properties].

A hybrid plasmid, pBE10 was constructed. It consists of DNAs of RSF2124 (ColE1 :: Tn3) plasmid and pUB110 plasmid of Staphylococcus aureus. The latter can be stably maintained in Bacillus subtilis. BamHI cleaved pUB110 was introduced into the BamHI site of transposon Tn3 and the resulting enlarged Tn3 (Tn2551) was transposed from pBE10 onto phage lambda and than to pMB9 (Tc) and RSF1010(Sm Su) plasmids. Restriction and heteroduplex analysis of pMB9 :: Tn2551(pBE21) and RSF1010 :: :: TN2551(pBE32) was carried out. Plasmids pBE10, pBE21 and pBE32 demonstrated some kind of molecular instability when introduced by transformation into Bacillus subtilis.

Bacillus subtilis↗
PubMed1981

[Pseudomonas aeruginosa phages whose DNA structure is similar to the DNA structure of phage Mu 1. III. The isolation and analysis of the hybrid phages D3112 and B39: localization of the immunity region and of certain genetic factors].

Several different hybrids have been isolated in a cross between heteroimmune Mu-like Pseudomonas aeruginosa bacteriophages D3112 and B39. Analysis of hybrid phage DNAs treated with specific endonucleases and heteroduplex studies have permitted to map the immunity region of phages in the left part of their genomes. Also, a ts-mutation in the wild-type B39 and some genetic factors influencing the growth of D3112 in P. aeruginosa strain PA0 (B39) and in bacteria harbouring RPL11 plasmid, have been localized. All recombinants studied have arisen by double crossingover and can be considered as substitutions of different continuous parts of the D3112 genome by fragments of the B39 genome. A comparison of distribution of non-homologous regions in B39 and D3112 genomes as well as locations of endonuclease-sensitive sites have given some unexpected results. In contrast to the B39-D3112 pair, the other pair of related Mu-like phages, B3-D3112, have no visible homology within the most part of their genomes.

Bacteriophage mu↗
PubMed1981

[Identification and the physicochemical characteristics of the extrachromosomal DNA from a Streptomyces antibioticus strain].

The data on centrifugation of DNA in the density gradient of caesium chloride-ethidium bromide, electron microscopy and electrophoresis of the DNA restricts from the satellite gradient band indicate that Str. antibioticus, the oleandomycin-producing organism possessed extrachromosomal cyclic DNA with the molecular weight of 21.3. 10(6) +/- +/- 0.3 10(6) daltons. The extrachromosomal cyclic DNA had 7 identification sites for endonuclease BamHI, 5 sites for PstI, 12 sites for PvuII, more than 14 sites for SmaI and non for endonucleases Eco RI and HindIII.

Chemical Phenomena↗
PubMed1981

[Physicochemical characteristics of transfer derepressed pKMR plasmids].

A group of transfer derepressed R factors (pKMR plasmids) was identified with the methods of conjugation and transformation in 2 antibiotic resistant strains of the dysentery bacillus, i. e. Shigella flexneri 3c and Sh. sonnei isolated from patients with acute dysentery. The antibiotic resistance in S. flexneri was controlled by plasmid pKMR 202-2 (Sm Tc Cm Km Su) with a molecular weight of 59 MD and that in Sh. sonnei was controlled by 2 plasmids, i. e. pKMR 203-2 (Ap Sm Tc Cm Km Su) and pKMR 203-3 (Ap Tc Cm Su) with molecular weights of 99 and 65 MD, respectively. When treated with restriction endonuclease BamH 1 plasmids pKMR 203-2 and pKMR 203-3 had each only one fragment with the similar molecular weight (7.7 MD). At the same time plasmid pKMR 202-3 differed from plasmid pKMR 202-2 only by the presence of an additional fragment BamH 1 with a molecular weight of 7.7 MD. The other 6 fragments of both plasmids had similar molecular weight. The data suggest that though plasmids pKMR 202-2 and pKMR 203-3 differ in their phenotypic features, they are closely related and possible belong to the same Inc-group. It was also shown that plasmid pKMR 202-2 segregated on transformation with formation of a nonconjugative plasmid pKMR 202-1 with a molecular weight of 16.6 MD.

Anti-Bacterial Agents↗
PubMed1980

[Pseudomonas aeruginosa bacteriophages with DNA structure similar to the DNA structure of Mu1 phage. II. Evidence for similarity between D3112, B3, and B39 bacteriophages: analysis of DNA splits by restriction endonucleases, isolation of D3112 and B3 recombinant phages].

It is found that bacteriophages B3 and B39 specific for Pseudomonas aeruginosa have the same genome structure as previously described phage D3112. On the right (S) end of their genomes a variable non-phage DNA is located (approximately 0.9-2.5 kilobases for different phages). It is probable that this variable DNa has its origin from different regions of bacterial chromosome. In genome of one of the phages, B3 phage, such variable DNA (not more than 150 base pairs) was found on the left end of DNA molecule. Isolation of a viable B3XD3112 recombinant phage and analysis of its genome with restriction technique and with studies of homo- and heteroduplex molecules had confirmed genetical relationship of B3 and D3112. Some essential non-homology of B3 and D3112 DNAs have been found on the right ends of genomes of the phages.

Bacteriophages↗
PubMed1978

[Construction and study of a new type of phage lambda DNA vector molecule].

A group of lambda mutants (mutants lambda 0) harbouring lesser number of EcoRI restriction sites on DNA molecules was selected. lambda3-1 recombinant (genotype lambdab221amgamma210Sr1lambda3+c-Px) was created by crosses of lambda02 phage with other lambda mutants. This phage DNA may be used as a vector molecule which makes it possible to select easily phages harbouring insertions of EcoRI DNA fragments. The maximal size of DNA fragment, the insertion of which would not decrease lambda3-1 viability, is 7.7 megadaltone. Lambda3-1 DNA has three regions heterological to lambda DNA, two of which probably include sites SRIlambda4 and SRIlambda5 and some juxtaposed genes. For example, Ptgene of lambda phage in juxtaposition with site SRIlambda4 is substituted by Px gene on the lambda3-1 DNA molecule.

Coliphages↗
PubMed1977

A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome.

A convenient procedure for the isolation of specific EcoRI-fragments of E. coli genome and their amplification on Km-resistance plasmid victor CK delta11 is described. The hybrid molecules were constructued in vitro using EcoRI-digestion, followed by ligation. Then appropriated E. coli strain was transformed with ligated DNA mixture and hybrid plasmids CK delta11-arg+, CK delta11-his+, CK delta11-thr+ and CK delta11-leu+ containing loci of E. coli genome were selected by molecular cloning. The hybrid plasmids obtained consisted of one EcoRI-fragment of initial plasmid CK delta11 and one respective specific portion of E. coli genome.

Arginine↗
PubMedDate not supplied

[Physicochemical characteristics of the Penicillium-bacterial virus type 5 and its nucleic acid].

Physico-chemical characteristics of PBV-5 virus and its nucleic acid were studied. Morphologically, the virus is a rectilinear polyhedron 270 A in diameter, without a process. The sedimentation constant of a virus particle is 117S, the buoyant density of the virus in cesium chloride density is 1.437 g/cm3. As shown by qualitative reactions with orcinol and diphenylamine, the virus contains DNA. The viral DNA preparation in CsCl gradient solution showed the buoyant density 1.719 g/cm3. Electron microscope examination of a PBV-5 DNA preparation showed the nucleic acid molecules to be single-stranded rings.

Centrifugation, Density Gradient↗
PubMedDate not supplied

[Construction of the synthetic genes for protein analogs of spider silk carcass spidroin 1 and their expression in tobacco plants].

To obtain transgenic tobacco plants expressing recombinant analogs of spider dragline silk spidroin 1, artificial 1f5 and 1f9 coding for spidroin 1 analogs were 3'-fused in-frame with the reporter lichenase gene. The Tr2' weak constitutive promoter of Agrobacterium tumefaciens T-DNA and the strong constitutive promoter of the cauliflower mosaic virus 35S RNA gene were used as regulatory elements. The expression cassettes were used to transform agrobacteria and then introduced in tobacco leaf disks. On evidence of Southern hybridization, transgenic plants each carried a single copy of a hybrid gene, which corresponded in size to the constructed one. Zymography and Western blotting revealed full-length hybrid proteins in leaf extracts of transgenic plants. The results testified that plants can maintain and express synthetic genes for spider silks and, consequently, may be used as a convenient producer of recombinant silk analogs.

Agrobacterium tumefaciens↗
PubMedDate not supplied

[A study of periodicity in the primary structure of spidroin 1 and spidroin 2 from spiders belonging to various species].

Sequences of spidroin 1 and 2 from spiders belonging to various species were analyzed by Fourier analysis. Specific periodical patterns were found in various segments of the sequences. These characteristic periodicities vary within the same proteins as well as between spidroin 1 and spidroin 2 sequences. It is possible that alterations in periodicity help to recognize contact sites between the molecules. Spidroins of 2 type have similar sequence structure consisting of four parts with a particular periodical pattern. These parts are a constant C-terminal part, a long-periodical part, a short-periodical part, and a constant N-terminal part.

Amino Acid Sequence↗
PubMedDate not supplied

[An analysis of the secondary structure of spider spidroins I and II belonging to different species].

We have analyzed the secondary structure of spidroin proteins of I and II types, related to spiders of different species. We used standard methods of secondary structure prediction NNPREDICT and JPRED and also analyzed the occurrences of oligopeptides with a preferred secondary structure with the help of the OLIGON program. We have demonstrated that local segments of the polypeptide chain can adopt alpha- and beta-conformations as well as the left-handed helix of polyproline II type. Periodical patterns found in the amino acid distribution indicate that there is a possibility of development of a macroscopic order accompanied by local conformational transitions.

Amino Acid Sequence↗
PubMedDate not supplied

[Construction of partial denaturation charts for DNA with random base distribution].

By measuring DNA temperature transition profiles the evidence was obtained that DNA of phages Tg9 and OB infecting Bacillus thuringiensis and Brevibacterium flavum respectively have a random base distribution. After alkaline treatment in the presence of 10 per cent formaldehyde at room temperature partially denatured molecules of phage Tg9 and OB DNA's with different degrees of denaturation were obtained. Using electron microscopy the maps of distribution of melted regions along these DNA's were obtained. The location of the main peaks on histograms did not vary with the increase of the degree denaturation and changes of pH values. The map of Tg9 DNA is characterized by four main peaks located at the positions 0.031, 0.075, 0.145 and 0.885 but the map of OB DBA is characterized by seven main peaks located at positions: 0.09, 0.20, 0.34 0.42,0.49, 0.80 and 0.88 from the left end (in relative units). The increase of denaturation up to a certain value did not change the half-width of the main peaks at the maps of these DNA's. It may be due to the presence in the DNA's some "heat stolle" sequences limiting early melting DNA regions.

Bacillus thuringiensis↗
PubMedDate not supplied

[Physico-chemical properties of several phages of Bacillus thuringiensis].

A study was made of biological and physico-chemical properties of phages of Bac. thuringiensis as well as of a number of parameters of nucleic acids isolated from these phages. The phages contain double-stranded DNA. Molecular weights of DNA from three phages--Tg9, Tg10 and Tg13 have been determined by two independent methods: by measuring the contour length of DNA, from the sedimentation constant and for DNA of phage Tg10 also by endonuclease EcRI hydrolysis. These methods gave similar results. On the basis of the temperature of DNA melting the content of GC pairs was found equal to 37.9, 33.4 and 35.1 mole% for DNA's of phage Tg9, Tg10 and Tg13, respectively. On the basis of measuring the intervals of DNA melting a conclusion was made that DNA of the Tg9 and Tg13 phage has a random distribution of base pairs, while DNA of phage Tg10 displays some clustering of base distribution along the molecule. It has been shown that restrictase EcoRI hydrolyses phage Tg10 DNA into 6 fragments of different molecular weights; DNA's of Tg9 and Tg13 phages are not hydrolyzed. A possibility of existance of phage Tg10 DNA in linear and ring forms has been established. The characteristics of phage particles have been determined by electron microscopy.

Bacillus thuringiensis↗