Biomedical subjects

V H Pérez-Luna

Publications and source records attributed to V H Pérez-Luna.

4 recordsLinked to original sources

PubMed2001

A surface plasmon resonance array biosensor based on spectroscopic imaging.

We have developed a multi-element transduction system which combines conventional SPR spectroscopy with one-dimensional SPR microscopy to create an effective platform for monitoring binding events on macro- or micro-patterned receptor arrays created on disposable sensor chips. This creates an effective platform for monitoring simultaneous binding events on each of the regions patterned with the receptors. This system has been specifically designed with commercially available components to allow relatively easy duplication. Furthermore, this system can use a proven, simple method to compensate for changes in the bulk index of refraction of the solution containing the analytes due to changes in temperature or solute concentration with simple modifications to the sensor chips alone. Preliminary results demonstrate how this system can be used to monitor several independent biospecific binding events simultaneously.

Biosensing Techniques↗

PubMed1999

Surface-grafted, environmentally sensitive polymers for biofilm release.

Controlling bacterial biofouling is desirable for almost every human enterprise in which solid surfaces are introduced into nonsterile aqueous environments. One approach that is used to decrease contamination of manufactured devices by microorganisms is using materials that easily slough off accumulated material (i.e., fouling release surfaces). The compounds currently used for this purpose rely on low surface energy to inhibit strong attachment of organisms. In this study, we examined the possible use of environmentally responsive (or "smart") polymers as a new class of fouling release agents; a surface-grafted thermally responsive polymer, poly(N-isopropylacrylamide) (PNIPAAM), was used as a model compound. PNIPAAM is known to have a lower critical solubility temperature of approximately 32 degrees C (i.e., it is insoluble in water at temperatures above 32 degrees C and is soluble at temperatures below 32 degrees C). Under experimental conditions, >90% of cultured microorganisms (Staphylococcus epidermidis, Halomonas marina) and naturally occurring marine microorganisms that attached to grafted PNIPAAM surfaces during 2-, 18-, 36-, and 72-h incubations were removed when the hydration state of the polymer was changed from a wettability that was favorable for attachment to a wettability that was less favorable. Of particular significance is the observation that an organism known to attach in the greatest numbers to hydrophobic substrata (i.e., H. marina) was removed when transition of PNIPAAM to a more hydrated state occurred, whereas an organism that attaches in the greatest numbers to hydrophilic substrata (i.e., S. epidermidis) was removed when the opposite transition occurred. Neither solvated nor desolvated PNIPAAM exhibited intrinsic fouling release properties, indicating that the phase transition was the important factor in removal of organisms. Based on our observations of the behavior of this model system, we suggest that environmentally responsive polymers represent a new approach for controlling biofouling release.

Acrylamides↗

PubMed1995

Investigating the relationship between surface chemistry and endothelial cell growth: partial least-squares regression of the static secondary ion mass spectra of oxygen-containing plasma-deposited films.

The relationship between endothelial cell growth and surface properties of plasma-deposited films (PDFs) was investigated using partial least-squares regression (PLS). PDFs of oxygen-containing precursors were prepared under various conditions, and bovine arterial endothelial cells (BAECs) were grown on these substrates. Secondary ion mass spectrometry (SIMS) in the static mode was used to characterize the surface chemistry of these substrates. The growth of BAECs on the PDFs was correlated to the positive and negative static SIMS spectra of the PDFs by PLS. A good correlation between the SIMS spectra of PDFs and endothelial cell growth was obtained. Qualitative information was also extracted from the multivariate model, giving some information as to the most important variables influencing BAEC growth.

Animals↗

PubMed1994

Developing correlations between fibrinogen adsorption and surface properties using multivariate statistics. Student Research Award in the Doctoral Degree Candidate Category, 20th annual meeting of the Society for Biomaterials, Boston, MA, April 5-9, 1994.

A multivariate model based on the partial least squares algorithm (PLS) was constructed in order to establish a correlation between the surface properties of common polymeric materials and the amount and retention of fibrinogen absorbed from a complex mixture. Surface characterization was performed by means of static secondary ion mass spectroscopy (SIMS), electron spectroscopy for chemical analysis (ESCA), and by contact angle measurements of several liquids on those materials. 125I-fibrinogen was adsorbed from a 1% plasma solution in buffer and the amount adsorbed after 2 h was determined. After 5 days of residence time in buffer, the adsorbed fibrinogen was eluted with a 1% solution of the surfactant sodium dodecyl sulfate (SDS). The percent of fibrinogen that remained on the surfaces after elution is referred to as fibrinogen retention. Correlations between surface properties and the amounts of fibrinogen adsorbed or fibrinogen retention were established. These models also show the most important variables that are related to the protein behavior on these surfaces.

Adsorption↗