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Biomedical subjects

V Hasková

Publications and source records attributed to V Hasková.

15 recordsLinked to original sources
PubMed1978

Human lymphoid cells release a lymphocyte-attaching and stimulating product.

In 4-h cultures human lymphoid cells release into culture supernatants substances capable of stimulating the proliferation of lymphocytes different at HLA system from cells which released the stimulating substances. Culture supernatant components responsible for stimulation are attached to lymphocytes allogeneic to their producers and on incubation with them can be removed from culture supernatants.

Animals↗
PubMed1978

Circulating antigen-antibody complexes in patients with immune complex disorders and following kidney transplantation.

The presence of circulating soluble complexes antigen-antibody was investigated by means of our simple test (1) in small serum samples of patients before and after kidney transplantation. We found a higher incidence of positive test results in patients with an original diagnosis of chronic glomeruleonephritis following transplantation in 37.1%, than before transplantation (in 28.5%). In both instances, however circulating immune complexes were less frequent than in a previously studied group of patients with chronic glomerulonephritis (66.6%), or particularly in glomerulonephritis cases with the characteristics of disease activity (100%) positivity. In patients suffering from pyelonephritis, a diseases not due to immune complexes, 18% of sera were positive. After transplantation the number of positive sera rose to 35.5% in 13 patients. The reasons for this finding are being analysed.

Antigen-Antibody Complex↗
PubMed1978

Simple method of circulating immune complex detection in human sera by polyethylene glycol precipitation.

A rapid test for detection of circulating immune complexes in a small serum sample was developed to facilitate clinical diagnosis of immune complex disorders. The test is based on a selective precipitation of soluble circulating complexes of antigen-antibody in 3.75% concentration of high-molecular polyethylene glycol. Precipitation is followed photometrically at 450 nm, 1 cm cuv. after 1 h incubation at room temperature. Comparison of E450 values in groups of patients with immune complex disorders, such as rheumatoid arthritis, systemic lupus erythematosus and glomerulonephritis, with healthy controls or patients with non-immunological disorders revealed highly significant differences. Sera of all patients with high clinical activity of disease exhibited positive reaction. In 121 human sera the results of this examination were compared with the results of C 1 q binding test. There was 73.5% agreement between the results of both methods. Our test is more rapid, suited for routine clinical use.

Antigen-Antibody Complex↗
PubMed1977

Changes in peripheral blood lymphocyte nucleoli in a follow-up of renal allograft recipients. Correlation with the response to PHA.

Changes in the morphology of nucleoli in peripheral blood lymphocytes and the reactivity to PHA were used in the immunological follow-up of renal allograft recipients. The increased numbers of "active" lymphocytes containing compact nucleoli or nucleoli with nucleolonemas, characteristic for cells actively synthesizing RNA, preceded 21 out of 28 rejection episodes. The results of the nucleolar test mathematically correlated with the results of the test of lymphocyte transformation to PHA. The described testing of lymphocyte activation according to the morphology of the nucleoli may serve as a rapid and economical method for the prediction of impending allograft rejection crises in the clinic.

Azathioprine↗
PubMed1977

Immunoprecipitation and radioimmunological studies of a lymph node activating factor released during contact of H-2-or HLA-different lymphocytes.

Immunoprecipitation studies showed that the supernatants from the 4-hour mixed cultures of allogeneic mouse lymphoid cells contained a specific component with the alpha2-globulin electrophoretic mobility which can correspond to, or to which a "lymph node activating factor" can be bound. In addition, during allogeneic cell culture serum proteins with the prealbumin mobility were released into the culture medium and in the control culture of cells from only one donor proteins with the albumin mobility predominated. In the supernatants from the 4-hour cultures of human HLA-identical and HLA-diferent lymphocytes beta2-microglobulin was determined by immunodiffusion and radioimmunologically.

Alpha-Globulins↗
PubMed1977

Effect of calf thymus histone and its fractions F2 and F3 on human lymphocyte stimulation by phytohaemagglutin.

The effect of exogenous calf thymus histone and its fractions F2 and F3 on blastic transformation of human lymphocytes stimulated by phytohaemagglutinin was investigated. It was found that histones can not only inhibit, but also potentiate phytohaemagglutinin-induced blastic transformation of lymphocytes. This effect depends on the amount of histones in the medium and most probably also on individual sensitivity of lymphocytes. The arginine-rich histone fraction (F3) displayed the greatest inhibitory effect on lymphocyte stimulation.

Animals↗
PubMed1976

Asimple test for immunogenicity of colloidal infusion solutions- the draining lymph node activation.

The increasing number of lymphocytes with nucleoli synthesizing RNA in the mouse lymph nodes, draining the site of injection of infusion solutions, was used as a marker for their immunogenicity. The percentage of "active" lymphocytes significantly increased 3 days after the administration of preparations based on bovine serum or human haemoglobin which were found immunogenic when testing for antibody formation. Such a reaction was not elicited in mice treated with Physiogel, Dextran and Duxon which do not cause any production of antibodies. The described test may serve as a rapid and economical assay for the immunogenicity of infusion solutions and other substances.

Animals↗
PubMed1975

Mouse lymph node activating factor released during contact of HL-A-different blood lymphocytes.

Ten-fold concentrated supernatants from the 4-hr mixed cultures of lymphocytes from two humans different at two haplotypes were inoculated s.c. into the hind footpad of mice. Twenty-four to forty-eight hours later, the cellularity and the synthesis of ribonucleic acid increased significantly in the popliteal lymph nodes draining the site of injection. The supernatants from cultures of HL-A-identical cells of cells different at one haplotype lacked this activity. The results suggest that human imcompatible lymphoid cells upon mutual contact liberate a soluble mediator capable of activating the draining lymph nodes; i.e., it is able to trap cells from the circulation and to stimulate them. The mediator is species-nonspecific; the activity produced by human lymphocytes can be tested on mice.

Animals↗
PubMed1975

Effects of various immunosuppressive drugs on the development of experimental renal infection in rats.

The effect of azathioprine, hydrocortisone and antihymocyte serum on the development of experimental colibacillary renal infection in rats was assessed by macroscopical findings in the kidneys, especially by determining the number of microbes in the infected organ, on day 7 after injection of the infectious agent. The greatest multiplication of bacteria and so the most pronounced macroscopical changes occurred after ALS treatment. There was statistically significant difference between ALS-treated group and the control group, whereas the differences between controls and the other experimental groups (Imuran, hydrocortisone) were non-significant.

Animals↗
PubMed1975

Transplantation models using the regional lymph node.

Several transplantation models, using the regional lymph node, to study the transplantation reaction in strictly defined and simple conditions were devised. Lymphoid cells were transplanted to inbred rats and mice at the sites drained by one regional lymph node; the experimental design was chosen so as to permit theoretically a one-way reaction, either the host-versus-graft (HVG) or the graft-versus-host (GVH) reaction. The changes in the lymph nodes draining the site of cell injection (weight increase, histology, lymphocyte activation) were very similar in both transplantation reactions. They were ascribed to a humoral mediator released upon the contact of lymphoid cells from two genetically different individuals. The direct demonstration of a mediator which is capable of activating the regional lymph node in vivo introduces some new aspects into the process of sensitization after transplantation.

Animals↗