SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'.
A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.
Base Sequence↗