PubMed Health⌕ Search

Biomedical subjects

V Hedouin

Publications and source records attributed to V Hedouin.

7 recordsLinked to original sources
PubMed2001

Morphine extraction in necrophagous insects remains for determining ante-mortem opiate intoxication.

To study the potential use of insects remains as toxicological indicators, we measured morphine in desiccated entomological materials using a radioimmunoassay method (Coat-A-Count Serum Morphine, Dade Behring, France) following enzymatic hydrolysis.First, enzymatic cuticle extraction of morphine (pronase digestion preceded by a 2h incubation with a dithiothreitol solution) was performed on various substrates (Calliphoridae puparial cases and desiccated adults, and desiccated pupae of Dermestidae) which were then tested to identify possible interferences with the radioimmunoassay procedure. This same procedure was performed on puparial cases and desiccated adults of Lucilia sericata (Diptera, Calliphoridae) previously reared on minced meat containing various morphine concentrations. Morphine was detected only in cuticle powder from insects reared on meats containing 100 and 1000mg/kg. Higher concentrations were measured in puparial cases. Rearings on psoas from eight heroin overdose victims confirmed previous experimental results. Remains of necrophagous insects, particularly puparial cases, are often preserved for a long time, and consequently can serve as late alternative specimens for toxicological analysis when suitable tissues are not available.

Animals↗
PubMed1999

Necrophilous insect succession on rabbit carrion in sand dune habitats in northern France.

Six freshly killed rabbit carcasses were exposed in different habitats in the coastal dune massif of Ambleteuse (northern France) during the spring seasons of 1996 and 1997. In total, 66 arthropod species were collected during the decomposition of these carcasses, and particular attention was paid to taxa of necrophilic significance. The pattern of insect activity was recorded and is discussed in relation to meteorological conditions. One significant feature in this study was the delay in initial oviposition by flies during 1996, which demonstrates the need for caution in estimation of postmortem interval by entomological techniques in early spring. Although daily temperatures may be favorable for adult fly activity, flies may be absent because of small population size and low nocturnal temperatures during this period.

Animals↗
PubMed1998

[Infection with hepatitis C virus in a prison environment. A prospective study in Loos-lez-Lille, France].

OBJECTIVES: The aim of this study was to assess the prevalence of hepatitis C virus (HCV) markers, and risk factors of contamination in a prison population. PATIENTS AND METHODS: Eight hundred and six prisoners were prospectively included, at the moment of their imprisonment, between December 1st 1995 and May 31st 1996. Each prisoner was included in a group "drug abusers" or "non drug abusers" based on a clinical examination. Serum anti-HCV antibodies were tested in each group. Other risk factors were also analysed (type of drug abuse, share of syringes and needles, blood transfusion, haemodialysis, and hemophilia). RESULTS: Among the 806 prisoners, 30.3% were anti-HCV positive. Four hundred and thirty nine prisoners (54.4%) were placed in the "drug abuser" group and 367 (45.5%) in the "non drug abuser" group. In the first group, 55.6% were anti-HCV positive (80% of the prisoners who were intravenous drug users and 10.8% for the others) and 4.2% were anti-HCV positive in the second group. CONCLUSIONS: Half of the prisoners entering our center were drug abusers and half were anti-HCV antibody positive. HCV infection is a major public health problem in prison.

Adolescent↗
PubMed1997

[The previous status].

The assessment of the condition prior to the traumatism by the expert is one of the main point of the medico-legal expertise and also one of most difficult. The aim of the report is to inform the settler, magistrate or insurance company, in a field in which he is not competent. He will have particularly to precise what has been the simultaneous evolution of the condition prior to the traumatism and of the traumatism. The consequences will be different according to the legislation.

Disease↗
PubMed1992

Variations in the glycoforms of serum alpha 1-antichymotrypsin in liver diseases and after liver transplantation.

1. Using crossed immunoaffinity electrophoresis with free concanavalin A in the first dimension, we studied the microheterogeneity of alpha 1-antichymotrypsin due to various glycoforms in sera from patients with various liver diseases and after liver transplantation. 2. Studies by isoelectric focusing and immunoblotting and by crossed immunoelectrophoresis without concanavalin A in the first dimension allowed us to show that there is no dramatic variation in electrophoretic heterogeneity of alpha 1-antichymotrypsin in the serum of patients with liver diseases or after liver transplantation when compared with that of normal subjects. Therefore the heterogeneity observed in crossed immunoaffinity electrophoresis is due to various interactions with concanavalin A. 3. The results were expressed as the ratio of concanavalin A non-reactive glycoforms plus concanavalin A weakly reactive glycoforms to concanavalin A reactive glycoforms, called R alpha 1-ACT. R alpha 1-ACT was significantly higher in patients with cirrhosis (n = 53) when compared with normal subjects (n = 30). The median R alpha 1-ACT was 1 (range 0.72-1.25) in normal subjects. It was 1.6 (range 1.18-3.02), 1.45 (range 0.65-4.12) and 2.24 (range 1.03-19) in cirrhosis of Child's grade A, B and C, respectively. There was a dramatic decrease in glycoforms with mostly biantennary glycans in some patients with Child's grade C cirrhosis. Serum levels of alpha 1-antichymotrypsin were lower than normal only in some patients with Child's grade C cirrhosis. 4. Among the patients with acute viral hepatitis studied (n = 17), five were studied longitudinally.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗