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Biomedical subjects

V I Dreval'

Publications and source records attributed to V I Dreval'.

At least 19 recordsLinked to original sources
PubMed1991

[Changes in the lipid and protein components of thymus cell membrane during lipid peroxidation].

Nonenzymatic lipid peroxidation in thymus cell plasma membranes was studied. The composition of lipid and protein components, intensity of fluorescence of the membrane probes (1-anilinonaphthalene-8-sulfonate, 4-dimethylaminochalcon, eosin, pyronin and rhodamine), fluorescence polarization of tryptophan residues of membrane proteins and quenching by acrylamide of intrinsic fluorescence of proteins were determined. Induction of lipid peroxidation by the Fe(2+)-ascorbate system caused changes in the composition and structure of lipids. This was paralleled with changes in the structural-dynamic organization of membrane proteins, transition of some peripheral proteins to the water phase and increased solubilization of integral proteins by Triton X-100.

Animals↗
PubMed1991

[Calcium ion binding by thymocyte plasma membranes].

The equilibrium in calcium ions binding to isolated cattle thymocyte plasma membranes within the concentration range 1.10(-5)--5.10(-4) M has been investigated. The plasma membranes has been shown to possess two kinds of Ca(2+)-binding sites with the association constants 2,35.10(5) and 7.64.10(2) M-1 and numbers of binding sites 3.45.10(-4) and 4.55.10(-3) mole.g-1 protein respectively.

Animals↗
PubMed1990

[The electrochemical stability of erythrocyte membranes].

The temperature measurements of the parameters of the medium kinetic pH curves when realizing the electrical breakdown of the erythrocyte membranes at the expense of the diffusional potential difference have been carried out. The energy values of the activation processes in antiport Cl-/OH- and potassium ions going out of the cells have been calculated. The values of activation energy of the given processes are different in the temperature range lying lower and higher 30-37 degrees C interval what the authors connect with the ratio change of contributions of lipid and protein components into kinetics of the processes. The values of the activation energy processes when erythrocyte mass was previously incubated with pro-oxidants, as well exposed to ultraviolet radiation have been obtained.

Electrochemistry↗
PubMedDate not supplied

[The effect of ionizing radiation on Ca ATPase of erythrocyte membranes].

Erythrocyte ghosts were irradiated with doses of 4 x 10(-3)-10(3) Gy. The activity of Ca(2+)-ATPase, the kinetic characteristics of enzyme reaction, the Hill's coefficient, the association constant and a number of La3+ linking centers were determined. The structural--functional peculiarities of Ca(2+)-ATPase changes under exposure to 4 x 10(-3) and 40 Gy of ionizing radiation have been established.

Calcium-Transporting ATPases↗
PubMedDate not supplied

[Change in the structure of rat liver mitochondrial membranes under the effect of ionizing radiation].

The effect of gamma-irradiation (under 0-10(3) Gy) on the intensity of lipid peroxidation, the microviscosity of annular and free lipids and the polarization of membrane proteins tryptophan fluorescence was studied in the outer and inner mitochondrial membranes. Some specific individual peculiarities of the mitochondrial membranes post-radiation changes were established.

Animals↗
PubMedDate not supplied

[Structure-functional states of liver mitochondrial membranes of rats exposed to irradiation].

The effect of the low dose gamma-irradiation (270 cGy--one-fold; 90 cGy per day during 3 days) on oxidative phosphorylation, lipid peroxidation, microviscosity of the annular and free lipids membrane, and membrane protein structural state was studied. The post-radiation influence on membrane functional activity and structural state in accordance with the irradiation regimes was established.

Animals↗
PubMedDate not supplied

[The effect of ionizing radiation in a wide dosage range on the structural-functional characteristics of the protein and lipid components of erythrocyte plasma membranes].

The erythrocyte ghosts were irradiated with doses of 4 x 10(-3) Gy-10(3) Gy. Changes in the membrane lipid microviscosity, membrane proteins' structural mobility, membrane surface potential and intensity of the lipid peroxidation processes were determined. It has been established that the features of membrane structural changes are characterised, by polyphase changes of examined parameters.

Blood Proteins↗
PubMedDate not supplied

[Radiation-induced changes in the structure of erythrocyte membrane proteins].

Structural changes in proteins of erythrocyte membranes induced by gamma-radiation at doses of 10-10(3) Gy were studied using the method of tryptophan fluorescence quenching by acrylamide. It was found that the exposure to ionizing radiation leads to a decrease in intramolecular dynamics of membrane proteins.

Erythrocyte Membrane↗
PubMedDate not supplied

[Exposure to ionizing radiation decreases hemoglobin binding to erythrocyte membrane].

The influence of gamma-radiation at doses 10, 10(2) and 10(3) Gy on binding of hemoglobin to the erythrocyte membrane by inductive--resonance energy transfer in the donor-acceptor pair anthracene-hemoglobin was studied. It was found that binding of hemoglobin to the erythrocyte membrane decreases with increasing dose of gamma-radiation.

Dose-Response Relationship, Radiation↗
PubMedDate not supplied

[Postradiation changes in Ca2+-ATPase and Mg2+-ATPase in thymocyte plasma membranes].

The rats were irradiated in the doses 1, 5, 4, 7 and 10 Gr and on the 1, 8, 15, 22 and 30 day after the irradiation activity of Ca(2+)-ATPase and Mg(2+)-ATPase and peroxidation lipids in the thymocytes was determined. It was found that postradiation changes in activity of Mg(2+)-ATPase were characterized by a higher sensitivity to the processes of lipids peroxidation as compared to Ca(2+)-ATPase.

Animals↗
PubMedDate not supplied

[The effect of ionizing radiation on the Ca2+, Mg2(+)-ATPase activity of plasma membranes].

A study was made of a change in Ca2+, Mg(2+)-ATPase activity induced by the effect of ionizing radiation (5-10(4) Gy) on a thymocyte plasma membrane suspension. The Michaelis' constant and maximum rate of enzymic reactions were determined. With a dose of 10(3) Gy the structural changes in Ca2+, Mg(2+)-ATPase were shown to reduce the affinity of the substrate to an active enzyme center and to decrease the rate of the enzyme/substrate complex degradation.

Animals↗
PubMedDate not supplied

[Effect of calcium on lipid peroxidation in plasma membranes].

Process of nonenzymatic peroxidation of lipids in the plasma membranes of thymocytes has been studied as affected by Ca2+. It is established that at calcium concentrations to 900 microM peroxidation gets more intensive; at higher concentrations of the intensity of this process falls.

Animals↗