Biomedical subjects
Publications and source records attributed to V I Dreval'.
Rats were irradiated at doses 1.5, 4.0, 7.0 and 10 Gy. After 1, 8, 15, 22 and 30 days the effect of blood plasma on activity of Ca(2+)-ATPase and Mg(2+)-ATPase in plasma membrane of thymocytes was investigated. It was found that the raise of irradiation dose leads to increasing of blood plasma effect on membrane-bound enzymes.
The effect of ionizing radiation at doses 10, 10(2), 10(3) and 10(4) Gy on Mg(2+)-binding plasma membrane proteins in thymocytes was studied. Marked changes in the Mg(2+)-binding protein structure-dynamic organization at doses above 10(3) Gy were found.
Rats were daily irradiated at doses 0.5 Gy in period of two weeks. The activity of Ca(2+)-ATPase, Mg(2+)-ATPase and the extent of lipid peroxidation in thymus were determined. The peculiarity of changing of enzymes activity demonstrates the dependence of Mg(2+)-ATPase on lipid peroxidation.
The change of Ca(2+)-ATPase and Mg(2+)-ATPase activity in plasma membranes of thymocytes irradiated with doses of 10(2), 10(3) and 10(4) Gy in the presence of Ca2+, Mg2+ and ATP was studied. Stabilizing effect of Ca2+ and Mg2+ on Ca(2+)-ATPase and ATP on Mg(2+)-ATPase under irradiation was established.
Thymocytes were irradiated by the doses of 4-1000 Gr. Activity of Ca(2+)-ATPase and Mg(2+)-ATP-ase, and lipid peroxidation were determined. It is supposed that changes in Ca(2+)-ATPase activity is connected with the adsorption of irradiation energy, while changes in Mg(2+)-ATPase activity are determined by effect of lipid peroxidation.
Plasma membranes of thymocytes in doses 10(2), 10(3) and 10(4) Gr were irradiated and subsequently illuminated by lambda = 445,5 nm or lambda = 518 nm light. Then the activity of Ca(2+)-ATPase and Mg(2+)-ATPase was determined. The photomodulation of the electron shell of calcium and magnesium atoms is a factor decreasing the effect of radiation on Ca(2+)-ATPase or Mg(2+)-ATPase.
Ca(2+)-ATPase from the meat cattle thymocytes plasma membranes in solutions 70% D2O, pH 4.0, 7.0 and 10.0 was irradiated with the doses of 10-10(4)Gr. It was shown that D2O produced stabilizing action on protein macromolecules. The activity of Ca(2+)-ATPase was changed under the influence of ionizing radiation in the presence of D2O.
Plasma membrane thymocytes were irradiated with doses of 10-10(4) Gy in the presence of 125 and 1125 mu mol of Ca2+ per 1 mg of membrane proteins. Fluorescence of 1-anilinonaphthalene-8-sulphonate and the degree of pyren eximerization were determined. The data obtained indicate that calcium can exert a stabilizing effect on the plasma membrane structure affected by radiation.
Thymocytes were irradiated with doses of 4 to 10(3) Gy. The binding of 1-anilino-naphthalene-8-sulphonate and Ca2+ to plasma membranes; viscosity and lipid peroxidation; Stern-Folmer constant; and the number of SH-groups of membrane proteins were determined. The structural changes in plasma membranes after irradiation of thymocytes were found to be cooperative.
Was determined quantity places and constant binding of calcium with plasma membranes thymocytes at rats, that was irradiation at doses 7 Gr. Was establish that to 22 days after irradiation the binding Ca2+ with membranes was return to control.
A thymocyte plasma membrane suspension was irradiated with a dose of 5 +/- 10(4) Gy. An increase in the rate of lipid peroxidation and a change in the phospholipid composition of membranes were revealed. The alteration of the plasma membrane structure was characterized by a change in the membrane charge and a decrease in the lipid viscosity.
Rats were exposed to radiation doses of 1.5, 4, and 7 Gy. Binding properties of 1-anilinonaphthalene-8-sulfonate, lipid viscosity, membrane protein structure, the activity of Ca(2+)-ATPhase and Mg(2+)-ATPhase in thymocyte plasma membranes were studied were investigated in health and on 1, 8, 15, 22, and 30 days. The character of post-radiation changes in the structure of thymocytes plasma membranes was determined.