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V I Evtushenko

Publications and source records attributed to V I Evtushenko.

42 records · Page 3Linked to original sources
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[Comparative study of low molecular weight RNA in various taxonomic groups].

snRNAs of rat, mouse, hamster, chicken, frog, Drosophila and yeast were studied. Mammals are characterized by the highest content of snRNAs. Most evolutionary conservative are 8SII and 8SIII RNAs which were present in all organisms studied. On the contrary 4.5S RNA was found only in mammals and birds. These data accord with the notion that the eldest in the family of snRNAs are U3 RNA involved in pre-rRNA processing and 8S polysomal RNA, while U5-U6 RNAs are more recent evolutionary achievements. In the frog at different stages of development the relative amount and the number of fractions of snRNAs differ significantly. In embryonic and regenerating rat liver the differences are less pronounced. Extra fractions of snRNAs were found in virus transformed chick fibroblasts and in ascite hepatomas of rat.

Animals↗
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[Hybridization of low molecular weight nuclear RNA with nuclear RNA and DNA restriction fragments].

Blot hybridization of 125I or 32P labeled 4.5S, U1 or U2 RNAs with EcoRI, HindIII, BamHI or SalG1 restriction fragments of high molecular weight DNA was performed. All these RNAs hybridized with fragments of ribosomal DNA and with 5.55-kb BamHI lines of rat. U1 ind 4.5S RNAs hybridized also with 3.6-kb HindIII lines. 125I labeled U1 RNA was hybridized with nuclear RNA in formamide. The hybrid molecules were formed which migrate in polyacrylamide gel as a broad peak slower than 28S rRNA. Our data indicate that snRNAs may participate in processing and/or splicing of hnRNA and in some other still poorly understood processes.

Animals↗