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Biomedical subjects

V I Fedenkov

Publications and source records attributed to V I Fedenkov.

13 recordsLinked to original sources
PubMed1990

[Solid-phase immunoenzyme analysis of apoprotein A-1].

The authors have optimized indirect solid-phase enzyme immunoassay for measuring human blood serum apoA-1. Immunochemical identification of the obtained apoA-1 and specific antibodies has been carried out, working regimens for the components and conjugates employed have been selected. Relationship between the method of preliminary treatment of the serum and the completeness of detected apoA-1 antigenic determinants is demonstrated. Normal blood serum apoA-1 level is 1.06 +/- 0.1 g/l in females and 1.05 +/- 0.1 g/l in male subjects.

Adult↗
PubMed1990

[Determination of the rate of utilization of glucose in a suspension of erythrocytes].

The authors suggest a new method for measuring the rate of glucose utilization in a cell (red cell) suspension, making use of interferometry. This method allows an easy examination of glucose utilization kinetics in time with but 1000 cells. Thermostatic control of the sample in the cuvette of the device permits detecting the temperature effects on glucose metabolism kinetics and thus define the temperature optimal for the process. A correlation was revealed between the point of structure phase transition in the red cell membrane and the optimal temperature for glucose utilization within the range of physiologic temperatures. This method may be useful in medical, biochemical, physiologic, and hematologic studies. The relative error of the technique is 3 percent.

Blood Glucose↗
PubMed1983

[Rheological properties of erythrocyte suspensions in acute myocardial infarct in the physiological temperature range].

A study was made over time of the relationships between the viscosity of red cell suspensions and temperature [eta (T)] in normal subjects and patients with acute myocardial infarction. In normal subjects, the curves eta (T) have a bend within the temperature range 37-40 degrees C, which apparently reflects the phasic transition. In patients with myocardial infarction, the bend on the curve eta (T) either disappears or is undemonstrable during the acute period within the temperature range indicated. During transition to the subacute period (after 7-10 days), the bend on the curves reappears and then only variation of its pattern follows. The changes seen in the viscosities of red cell suspensions or red cell "shades" in the phasic transition area might be used for defining the stages, the time course, and prognosis of myocardial infarction.

Adult↗
PubMed1979

[Mechanisms of the structural changes in erythrocyte membranes under the action of a shift in pH].

The effect of pH shifts (5--10) on erythrocyte "ghosts" has been studied using the IR spectroscopy. The process of regulation has been shown to take place within pH 7.15--8 range involving both protein (transition testified by absorption band changes 1520, 1540, 1635, 1645, 1585, 1696, 3305 CM-1) and lipid membrane components testified by absorption band splits 1745, 1470, 1380, 720 AND 1242 cm-1. The transition from a lesser to a higher regulating state of membrane structure happens unevenly, since the above changes take place within the narrow pH 7.15--8 interval, near the homeostase limit, being never observed at pH 5--7.15 or 8--10. It is supposed that the increase in regulation of both protein, phospholipid and lipid membrane fields is of phasic nature which presumably constitutes the basis for pH action mechanism as a regulation factor.

Animals↗
PubMed1977

[Microthermal method of separating red cells into groups according to age].

The microthermal method of red blood cells' division according to their age is based on the formation of a temperature gradient with the aid of microthermoresistor. The red blood cells of different age fractions are consecutively localized and selected in the zone of microthermoresistor. They differ from one another according to their biochemical indices. The microthermal method of erythrocytes' division offers a possibility of differential studying the blood cells.

Animals↗