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V I Iliukhin

Publications and source records attributed to V I Iliukhin.

At least 19 recordsLinked to original sources
PubMed2005

[Molecular-genetic approaches to diagnosis and intraspecific typing of causative agents of glanders and melioidosis].

Pathogenic Burkholderia--Burkholderia mallei and Burkholderia pseudomallei--are causative agents of glanders and melioidosis, severe infectious diseases of man and animals. They are regarded as potential agents of bioterrorism. The existing bacteriological and immunological methods of identification of B. mallei and B. pseudomallei are not efficient enough for the rapid diagnosis and typing of strains. Described in the paper are molecular methods of detection of the agents by PCR, hybridization and strain typing made on the basis of bacterial total cell protein profiles, RAPD, ribotyping as well as of plasmid and DNA microrestriction analyses.

Burkholderia mallei↗
PubMed2005

[Comparative analysis of total cell protein electrophoregram of pathogenic Burkholderia].

Whole-cell proteins of 22 strain of Burkhoderia pseudomallei, including 13 B. mallei, 5 B. cepacia strains and 14 strains of opportunistically pathogenic Pseudomonas defined by 1D SDC-PAAG electrophoresis. Electrophoregrams contained 35 to 45 protein fractions sized 19 to 130 kDa, which were highly reproductive. On the basis of computer-aided comparative analysis of protein patterns the interspecies and intraspecies grouping of studied microorganisms was made. The cluster analysis of the similarity matrix of protein spectra made it possible to allocate two groups of strains at the level of similarity of 78%. Group I was formed by Burkholderia species that previously belonged to the II RNA-DNA homology group of Pseudomonas: B. pseudomallei, B. mallei, B. cepacia. All Pseudomonas species were added to the 2nd Group: P. aeruginosa, P. stutzeri, P. testosterone, P. fluorescens, P. putida, P. mendocina. Four phenons were isolated among the strains of B. pseudomallei and 2 phenons--among the strains of B. mallei at the threshold similarity level (89%). The authors conclude that the comparative analysis of electrophoregrams of whole-cell proteins can be useful in the identification and typing of pathogenic Burkholderia.

Bacterial Proteins↗
PubMed2004

[Use of PCR for identification of Burkholderia mallei].

Stimuli of glanders belong to the potential agents of biological terror. The possibility to use various primers in the identification of B. mallei was investigated and the significance of polymerase chain reaction (PCR) was defined within the scheme of laboratory glanders diagnosis in the offered paper. The constructed amplifying test-systems can be used to detect the glanders both in the environmental objects contaminated with B. mallei and in experimental clinical material.

Base Sequence↗
PubMed2003

[Identification of the causative agents of glanders and melioidosis by polymerase chain reaction].

Burkholderia mallei and B. pseudomallei are causative agents of glanders and melioidosis, respectively, i.e. severe and fatal infection diseases of man and animal. The computer-based analysis of the 23S rRNA gene sites was used for selecting the primers. Two pairs of primers were chosen for the identification of B. mallei and Bpseudomallei. DNAs from 48 B. pseudomallei and 15 strains of B. mallei, unlike from other geterological bacteria, were positively amplified. Therefore, the method of polymerase chain reaction can be used in laboratory diagnosis of glanders and melioidosis.

Animals↗
PubMed2002

[Burkholderia thailandensis: biological properties, identification and taxonomy].

Burkholderia pseudomallei-like microorganisms have been isolated from soil and water in regions with endemic melioidosis. These strains have biochemical and antigenic profiles identical to melioidosis agents, except that they differ by virulence and L-arabinose (vir-, ara+). There are minor differences between these species by rRNA sequence. DNA hybridization and, more so, positive transformation of DNA auxotrophic mutants of B. pseudomallei by cell lysates of B. thailandensis and B. mallei confirmed the homology of these species' genomes. These members of the Burkholderia genus (pseudomallei, mallei, and thailandensis) can be regarded as a supraspecies taxon: pseudomallei group. B. thailandensis strains are not virulent for guinea pigs and slightly virulent for golden hamsters. Immunization with live cultures of B. thailandensis protected more than 50% guinea pigs challenged with 200 LD50 B. pseudomallei 100. B. thailandensis is suggested as a potential melioidosis vaccine.

Animals↗
PubMed1998

[Isolation and primary characteristics of a Pseudomonas (Burkholderia) pseudomallei plasmid].

Plasmid screening of reference Pseudomonas pseudomallei strains isolated from patients and animals revealed cryptic plasmids with different molecular weights in 30 strains. Plasmids were investigated by restriction analysis and DNA-DNA hybridization. Cryptic plasmids were denoted as pPM1, pCM2, pCM3, and pCM4. The presence of plasmids in melioidosis agent permits their use for intraspecies typing of strains and for genetic studies.

Animals↗
PubMed1997

[Effect of medium temperature and pH on the sensitivity of pathogenic pseudomonads to chemotherapeutic agents].

Antibiotic susceptibility of four Pseudomonas species i.e. P.aeruginosa, P.cepacia, P.mallei and P.pseudomallei was studied under conditions of different temperature and pH of the medium (within the physiologically possible deviations). It was shown that along with the general tendencies (lowered MICs at the medium alkalization) there were obvious species differences in the effect of the physicochemical factors on the Pseudomonas antibiotic resistance. The results of the study may to a certain extent serve as an explanation of the lack of coincidence of the chemotherapy efficacy with the data on the drug in vitro estimation.

Anti-Bacterial Agents↗
PubMed1996

[Recovery rate in chemotherapy of glanders].

It was shown that out of all the studied methods for the estimation of the recovery rate in animals with experimental malleus the solid-phase enzyme immunoassay with parallel growing of the test material and subsequent isolation of the antigens and L-forms proved to be the most efficient. In addition to the routine criteria of the recovery the malleinic test was a valuable diagnostic means for the estimation of the recovery rate and the efficacy of the antibacterial drugs in a model of guinea pigs. The immunological investigations revealed a dependence of the host sanation on the term of the animal chemotherapy. There was detected no L-transformation of Pseudomonas mallei during the treatment of the animals with experimental malleus with combinations of sulfanilamides with trimethoprim (biseptol and sulfaton) or rifampicin which was evident of their high sanation activity and safety in comparison to sulfamonomethoxin used alone.

Animals↗
PubMed1995

[Laboratory control of effectiveness of chemotherapy in melioidosis].

An undoubtable role of the melioidosis germ persistence in the form of L-variants with the development of DTH in the relapses of the disease was stated. Serological reactions such as CFT and PHAR were shown to be of the diagnostic value but it was by far lower than the allergotest. No correlation between the antibody titers in the tested sera and the isolation of the bacterial or L-variants of the melioidosis germ was observed. The criteria of the recovery from melioidosis were: stable normalization of the patient temperature, positive time course of the body weight changes, negative tests for the type and morphologically changed forms of the melioidosis germ and no allergic reactions.

Animals↗
PubMed1991

[Problems of resistance of the causative agent of melioidosis to antibiotics].

It was shown that the pathogen causing melioidosis was highly resistant to antibiotics including beta-lactams. Antibiotic sensitive mutants of P. pseudomallei were isolated after mutagenesis induced by nitrosoguanidine. Permeability for 3H-tetracycline and tetracycline sensitivity of the mutant cells was respectively 3 and 20 times as high as those of the initial parent strain. Gas liquid chromatography revealed quantitative and qualitative changes in separate sugars of the lipopolysaccharide structure in antibiotic sensitive mutants as compared to the initial strain.

Anti-Bacterial Agents↗
PubMed1991

[Sensitivity of Pseudomonas to currently used antibacterial drugs].

Sensitivity of 184 strains of four Preudomonas species that are of importance from the medical viewpoint was studied. It was shown that all the studied strains were resistant to cefazolin and ampicillin. Sulfomonomethoxine was active only against P. mallei. Gentamicin inhibited the growth of all the strains of P. mallei and 60 per cent of the strains of P. aeruginosa. Among the four studied strains, the causative agent of glanders was the least resistant to the chemotherapeutic drugs. Eleven out of the 16 studied drugs were active against P. mallei. The strains of P. pseudomallei, the organism causing melioidosis were sensitive to 6 drugs: ceftazidime, imipenem, doxycycline, minocycline, chloramphenicol and biseptol. The dime, ceftriaxone, cefsulodin, cefoperazone, cefotaxime, piperacillin and imipenem. The strains of P. cepacia were the most resistant to all the chemotherapeutic drugs studied and only biseptol was active in the concentrations attained in blood of patients (MIC50 12.5 mg/l). 10 to 70 per cent of the strains were sensitive to ceftazidime, imipenem, doxycycline, minocycline and rifampicin. Therefore, imipenem and ceftazidime were the most active drugs with respect to the causative agents of pseudomonoses. Biseptol, doxycycline and minocycline are also worthy note.

Anti-Bacterial Agents↗
PubMed1983

[The nature of changes in the antigenic spectrum of the causative agent of melioidosis during animal passage].

The comparative analysis of the antigenic spectra of Pseudomonas pseudomallei museum and subcultured strains, carried out by the method of immunoelectrophoresis, has revealed that, along with an essential increase in the virulence of P. pseudomallei for white mice and changes in the morphology of colonies, a decrease in the amount of detected precipitinogens occurs in the process of subculturing. The immunoelectrophoregrams of the subcultured variants show the absence of antigens 5, 6 and the simultaneous increase of the production of antigen 8, one of the components of mucoid (in the pseudocapsule).

Animals↗
PubMed1981

[Antigenic structure of Pseudomonas pseudomallei].

The antigenic scheme has been proposed on the basis of the immunoelectrophoretic study of aqueous-saline extracts from acetone-dried microbial cells belonging to 29P. pseudomallei strains. The proposed scheme consists of 19 precipitinogens; of these, 13 have been shown to be common to all strains under study, the remaining precipitinogens being variable. All antigens are characterized by their charge and the degree of mobility in the electric field, their molecular weight, thermal resistance and chemical nature. In future this scheme is likely to be used for reference in the study and isolation of different antigenic structures of the infective agent of melioidosis.

Antigens, Bacterial↗
PubMed1980

[Postinfection immunity in melioidosis].

Experiments on the reinfection of laboratory animals with melioidosis revealed that guinea-pigs and white mice developed pronounced resistance to the disease, accompanied by the objective [corrected] signs of immunologic transformation (allergization, the presence of specific humoral antibodies, and increase in the phagocytic activity of the cells of the macrophage system). In golden hamsters, highly sensitive to infection with Pseudomonas pseudomallei culture, the process of the "all or nothing" type occurs, and the degree of resistance depends on the fluctuations of the individual sensitivity level. No phenomena of immunologic transformation were observed in reinfected golden hamsters.

Acute Disease↗