[Social conflict and tumor growth].
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Aggression↗
Biomedical subjects
V I Kaledin
Publications and source records attributed to V I Kaledin.
[Effectiveness of a liposomal form of rifampicin in the treatment of experimental tuberculosis in mice].
The therapeutic effectiveness of rifampicin as a free form and a component of multilamellar phosphatidylcholine cholesterol liposomes was studied on a model of generalized tuberculosis in BALB/c mice. Rifampicin as a liposomal form was found to have no advantages over its free form. Possible mechanisms of the phenomenon and prospects for using liposomes in the chemotherapy of tuberculosis are discussed.
Animals↗
[Effects of indomethacin and BCG vaccine on growth of transplanted tumors in mice].
Continuous administration of indomethacin (IM) in drinking water (20 micrograms/ml) causes moderate inhibition of the growth of transplantable tumors in control mice but significantly increases the antitumor effect of BCG vaccine inoculated in combination with transplanted tumor cells. Hepatoma 29, insensitive to the vaccine becomes highly sensitivity to it under IM administration.
Adenocarcinoma↗
[Reciprocal differences in the susceptibility to induction by ortho-aminoazotoluene of liver and vascular tumors in A/HeJ and CC67BR/Mv mice].
When administered subcutaneously, ortho-aminoazotoluene induces predominantly liver tumours in A/HeJ mice but vascular tumours (located mainly in the interscapular brown fat pads) in CC57BR/Mv mice. Liver tumours are more frequent in females, while vascular tumours--in males. Under crossing, the susceptibility to induction of the both types of tumours in male F1 hybrids is inherited intermediately, whereas in females the resistance to induction of vascular tumours predominates.
Animals↗
[Comparative evaluation of the glycosaminoglycan composition in plasma membranes of normal and transformed mouse liver cells].
Using electrophoresis in agarose gel, a comparative study of composition of membrane-bound glycosaminoglycans (GAG) from normal mouse liver cells and from o-aminoazatoluene-induced mouse hepatoma cells was carried out. Differences in the composition and localization of GAG were revealed. The plasma membrane fraction of hepatoma cells contained no GAG; the bulk of GAG (approximately 98%) was localized in the plasma membrane free fraction. Within this fraction GAG contained no heparan sulfates with a high electrophoretic mobility that were detected in plasma membranes of normal liver cells. The possible involvement of proteoglycans bound to cell surface in transmembrane signal transfer is discussed.
Animals↗
[Different predisposition to the development of spontaneous and induced liver tumors in mice of different strains and their hybrids].
No correlation between the sensitivity to induction of liver tumours by ortho-aminoazotoluene and predisposition to spontaneous development of these tumours was found in mice. Under crossing the predisposition to spontaneous tumours was inherited dominantly, while sensitivity to their induction was inherited by the intermediate type. Spontaneous hepatomas were more frequent in males, whereas induced onesin females of the same genotype.
Animals↗
[Suppression of tumor growth in the liver by cis-diamminedichloroplatinum as large oligolamellar liposomes prepared by freezing and thawing].
A/HeJ mice with experimental metastases of HA-1 tumor in the liver and other organs were given therapeutic doses of cisplatin, free or encapsulated in phosphatidylcholine cholesterol freeze-thawed liposomes, intravenously. Free cisplatin treatment was found to decrease the growth rate of liver metastases by half and to extend survival by 1.5 times as compared to controls. The inhibitory effect of liposome-encapsulated cisplatin on hepatic metastases growth was more pronounced than that of the free drug although it weakly affected metastatic growth in other organs.
Animals↗
[Effectiveness of cycloplatam in the free form and included in liposomes during metastasis of GA-1 tumors in A/He mice].
The 6-10-fold freezing and thawing of routine multilamellar phospholipid vesicles in cycloplatam solution give rise to liposomes which entrap in their aqueous phase up to 10 mg of the drug per 100 mg of lipids. When injected intravenously to mice with HA-1 tumour metastases in the liver, liposome-encapsulated cycloplatam increased their survival rate by 112-123%, whereas free cycloplatam--only by 39-61% as compared to the control. The intramuscular transplants of the tumour were affected similarly by both liposome-encapsulated and free cycloplatam (the tumour growth was inhibited by 67.1 and 75.6%, respectively).
Animals↗
[Decrease in the antimetastatic effect of vinblastine administered in liposomes].
The model of experimental metastases in the HA-1 tumour in the liver of A/He mice was used to show that the anti-tumoural effect of cis-dichlorodiamminoplatinum being used in the liposomes increases, while that of vinblastine (VB) decreases. It is suggested that the low activity of liposome-encapsulated VB as to its influence on the tumour growth in the liver is a result of preferable uptake of liposomes by Kupffer cells and hepatocytes from where VB cannot diffuse into tumour cells since it binds to intracellular tubulin.
Animals↗
[Increased content of nucleotide sequences in transcriptionally active DNA and poly(A)+-mRNA of the rat liver and a rise in its translation activity as affected by inducers].
Cortisol and 3'-methyl-4-dimethyl-amino-azobenzene induce an increase in the content of repeated sequences (RS) in transcriptionally active (TA) DNA, while the content of respective RS in potentially active DNA fractions enriched with regulatory regions of the genome decreases. RS content in induced poly A+-mRNA also rises, as determined by the nature of hybridization of respective c DNA with total DNA. The translation of induced poly A+-mRNA rises essentially, with the qualitative distinctions in in vitro synthesized protein product spectrum being absent. Inducible RS with unstable chromatine conformation are thought to provide a universal system of rapid response of the genetic apparatus to extreme situations, serving as transcription intensifiers in TA DNA and as translation intensifiers in induced poly A+-mRNA.
Adrenal Glands↗
[Detection of DNA sequences sensitive to glucocorticoids in the thymus of AKR mice and their inactivation in thymoma].
A high increase in transcriptionally active DNA fraction (TA DNA) has been detected in the thymus of young AKR mice and in thymoma. Hybridization of TA DNA with [32P] cDNA synthesized on poly A+-mRNA of cortisol-induced animals has shown that TA DNA of young AKR mice contains 40 times as much cortisol-activated repeated sequences (RS) as that of thymoma. The decrease of cortisol-activated (RS) in AKR thymoma TA DNA was found to be the result of their transition into transcriptionally inactive (TI) DNA. It is concluded that chromatin conformation changes take place in cortisol-activated RS DNA region of AKR mouse thymus before transformation. Unstable conformation of RS DNA in the thymoma can possibly promote their transition into TI DNA.
Animals↗
[Selective adhesion of tumor cells to the endothelium of the target organ in metastasis].
The fragments of cell plasma membranes of two lines of metastatic tumour of A/He mice (lung adenocarcinoma--LA-cells and hepatoma HA-1--HA-cells) were used to prepare "hybrid" vesicles (LA- and HA-liposomes). Incorporation of the fragments of LA-cells into the bilayer vesicle increased the binding of LA-liposomes with lung almost by an order of magnitude, their trapping by liver being noticeably decreased. HA-liposomes were retained in the liver in larger amounts than the model phospholipid liposomes. It is assumed that the specificity of metastatic spreading into an organ is associated with the interaction of the plasma membrane of the tumour cells with the apical membrane of the target organ endothelium.
Adenocarcinoma↗
[Possible mechanism of the recognition of the target organ by tumor cells during metastasis].
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Adenocarcinoma↗
[Ratio of spontaneous and o-aminoazotoluene-induced hepatocarcinogenesis in mice].
No correlation was found between the frequency of spontaneous and o-aminoazotoluene (o-AAT)-induced hepatic tumours in mice. High susceptibility to tumour induction with o-AAT in mice both genetically susceptible (strains CBA and A/He) and resistant (strains DBA/2J and DD) to spontaneous hepatocarcinogenesis was detected. The CC57BR mice predisposed to spontaneous hepatomas were insensitive to their induction with o-AAT. The model described may be helpful in studies of the nature of the initiation and promotion stages of hepatocarcinogenesis.
Animals↗
[Characteristics of alpha-fetoprotein synthesis in the early stages of hepatic carcinogenesis in mice with a various sensitivity to ortho-aminoazotoluene].
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Animals↗
High level of alphafetoprotein (AFP) and persistence of hemopoiesis in the liver of nude mice.
Using indirect immunofluorescence, radial immunodiffusion and radioimmunoassay methods, changes in the number of AFP-synthesizing cells in the liver and in the serum AFP concentration during postnatal development were studied in C3H/HeSto and BALB/cLacSto mice (normal and nu/nu (nude) mutants). From the age of 2 weeks, serum AFP levels were significantly higher in nude mice as compared to normal mice. AFP-positive cells also disappeared later from the liver of nude mice compared to normal. Morphological analysis and liver smear cell counts demonstrated that hemopoietic foci persist in the liver of nude mice during their entire life. The relationship between AFP synthesis and hepatic hemopoiesis is discussed.
Animals↗
[Increase in the concentration of alpha-fetoprotein in the blood of mice following a single dose of cyclophosphane].
Activation of extramedullar hemopoiesis in the liver together with an appreciable elevation of the alpha-fetoprotein (alpha-FP) level in the blood were observed in CBA, C3H/HeJ, DD, C57BL/6 and CC57BR/Mv mice after a single injection of cyclophosphane in a dose of 200 mg/kg bw. The alpha-FP concentration reached maximum days 2-4 after cyclophosphane administration, amounting of 290-560 ng/ml which surpasses 2-5-fold the basal level of the protein. In mice with an earlier serum alpha-FP elevation (CBA, C3H/HeJ and DD), the liver hemopoiesis also started earlier than in those with a delayed alpha-FP maximum (C57BL/6 and CC57BR/Mv).
Animals↗
[Mechanism of a decrease of inducibility of tyrosine aminotransferase by carcinogenic aminoazo dyes. Absence of direct influence of o-aminoazotoluene on the enzyme molecules].
The effect of o-aminoazotoluene (OAT) on the activity of tyrosine aminotransferase (TAT) from mouse liver cytosol under its incubation in the presence of the systems providing for the metabolic activation of the cancerogen (liver microsomes and NADPH2) and dephosphorylation of TAT molecules (light mitochondria and ATP) was studied. It was shown that OAT has neither direct nor indirect (via the phsophorylation--dephosphorylation systems) effect on the activity of TAT. It was concluded that the decrease of TAT induction by hydrocortisone in vivo resulting from injection of OAT to the mice is not due to the direct influence of the cancerogen on the enzyme molecules.
Animals↗