PubMed Health⌕ Search

Biomedical subjects

V I Kapliev

Publications and source records attributed to V I Kapliev.

9 recordsLinked to original sources
PubMed1992

[Stability of bacterial aerosols in static conditions].

The influence of acid exopolysaccharides of some Pseudomonas species on the viability and fractional disperse composition of Salmonella marcescens-9 aerosol under static conditions has been studied. Acid exopolysaccharides have been shown to form membranes around bacteria. These membranes, due to their adsorptive and water-retaining properties, seemingly enhance the viability of bacteria in aerosol, both in the process of its generation and during its suspension in the air under static conditions with different values of relative humidity.

Aerosols↗
PubMed1991

[The role of the surface antigens of Pseudomonas pseudomallei in the pathogenesis of melioidosis].

As established with the use of electron-immunochemical techniques, glycoprotein antigen 6 is the outer membrane component of P. pseudomallei cell wall, while glycoprotein antigen 8 is localized on the cell surface as a capsule-like formation. Antigen 6 plays no perceptible role in the realization of the pathogenic properties of the infective agent, but serves as a reliable sign in the differentiation of P. pseudomallei strains into serovars. Subcultures, defective in the synthesis of antigen 8, have sharply reduced virulence for laboratory animals. As revealed in this study, the pathogenetic action of antigen 8 is linked with its pronounced antiphagocytic function. Thus, antigen 8 is considered to be one of the key pathogenicity factors of P. pseudomallei.

Animals↗
PubMed1990

[The morphological characteristics of mucoid variants of Pseudomonas pseudomallei].

Two types of mucoid colonies formed by P. pseudomallei cultivated on solid culture media have been studied by the method of electron cytochemistry. The intercellular material of primary mucoid colonies has been found to positively react with ruthenium red, which is indicative of its polysaccharide nature. Slime is the product secreted by P. pseudomallei and participates in the formation of the pseudocapsule and colonies. The intercellular material seems to be a sum of biopolymers based on the products of destroyed bacterial cells.

Bacterial Proteins↗
PubMedDate not supplied

[The determination of the location of the antigens of the causative agent of melioidosis at the ultrastructural level by using monoclonal antibodies].

The indirect immunoperoxidase method with the use of monoclonal antibodies has been employed to determine the localization of antigens of melioidosis agents. The formed reaction products were then detected by the electron microscopic analysis. The most important in melioidosis pathogenesis antigens 6 and 8 (AG 6 and AG 8) were determined to localize on the cell wall and in the capsule-like mucous layer, respectively. Monoclonal antibodies to AG 8 due to their marked cross-linking properties promote stabilization, preservation and detection of labile melioidosis agent. At the same time the mucous layer prevents monoclonal antibodies to AG 6 to access to the corresponding antigen, localized on the cell wall and makes its detection difficult.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Ultrastructural immunochemical study of pathogenic Burkholderia capsule].

The capsular structures of Burkholderia pseudomallei, B. mallei, B. cepacia and their avirulent noncapsular mutants were studied with the use of electron ahd immunocytochemical techniques. For this purpose, antimelio-idosis monoclonal antibodies (McAb) G11 and 1 G2, epitope-aimed at capsular glycopyotein of 200 kD and outer-membrane proteins of 42 and 39 kD, were used. As revealed in this study, the typical causative agents of melioidosis and glanders formed the capsule and exhibited high virulence due to the antiphagocytic activity of 200 kD glycoprotein, whose epitopes were found to be incorporated into the capsule, in contrast to avirulent variants and B. cepacia, found to have no such structure. The recognition of the membrane determinants of McAb 1 G2 on the outer-membrane surface of the non-capsular variants of microbes known to be the causative agents of melioidosis and glanders was indicative of absence of the capsule in these microbial cells. These data concerning the role of 200 kD antigen in virulence, its structural and functional characteristics may be efffectively used in the study of the pathogenetic mechanisms of melioidosis and glanders, as well as in the construction of preparations for their immunodiagnostics and prophylaxis.

Arabinose↗
PubMedDate not supplied

[The level of spontaneous phage production and sensitivity to melioidosis phages of museum cultures of Pseudomonas pseudomallei].

The phage-producing activity and sensitivity of museum cultures of a melioidosis agent to melioidosis phages have been studied. Most cultures show spontaneous phage-production, though its level differs in strains. Some of the melioidosis cultures demonstrate lack of phages. The fact that these strains have different sensitivity to chloroform lysate of cultures producing phages indicates their possible lysogenic state. Electron microscopy has shown the presence of at least tow morphological types of phages in P. pseudomallei C-141. These data confirm existence of polysogeny in the melioidosis agent.

Bacteriophage Typing↗
PubMedDate not supplied

[The isolation and characteristics of cloned strains of Pseudomonas pseudomallei phages].

On the basis of museum strains of the melioidosis agent Pseudomonas pseudomallei, 14 pure lines of bacteriophages were isolated belonging to morphological groups IV and V according to the classification by A. S. Tikhonenko. These phages show a broad bacteriolytic range as far as museum P. pseudomallei cultures are concerned. It was determined that some phages isolated from the same melioidosis culture and belonging to the same morphological type display different bacteriolytic activity. It is demonstrated that some strains of melioidosis agent are characterized by polylysogeny.

Bacteriological Techniques↗
PubMedDate not supplied

[Thermostable Pseudomonas pseudomallei hemolysin].

The chemical nature of thermostable P.pseudomallei hemolysin has been determined. This substance is glycolipid, synthesized by bacteria at the stationary phase of growth under the conditions of the prevalence of the source of carbon over the source of nitrogen in the nutrient medium. The hemolytic activity of thermostable hemolysin has a delayed character and is more pronounced in P.pseudomallei R-forms.

Burkholderia pseudomallei↗