PubMed Health⌕ Search

Biomedical subjects

V I Konenkov

Publications and source records attributed to V I Konenkov.

At least 37 records · Page 2Linked to original sources
PubMed1994

[The DNA typing of the HLA-B27 gene among individuals with joint lesions].

To determine the HLA-B27 gene subtypes associated with joint diseases we examined 55 patients (35 with ankylosing spondylarthritis (AS), 13 with rheumatoid arthritis (RA), 5 with Reiter's syndrome, 2 with psoriatic arthritis). DNA amplification combined with sequence specific oligonucleotide hybridization were used to identify six HLA-B27 gene alleles. In the AS patients group the frequency of HLA-B2705 variant was found to be different significantly from that in healthy West Siberia Caucasians and distribution of HLA-B27 gene allele variants among AS patients in East Russia differed from that in West regions of Russia. DNA typing data can be useful together with other criteria for diagnosis of AS in a early onset.

Adult↗
PubMed1994

[A clinico-immunological analysis of clinical variants of connective tissue dysplasia].

The clinico-immunogenetic analysis of 47 patients with Marfan's syndrome and 83 patients with chest deformities combined with valvular prolapses and other connective tissues dysplasias elucidated a close relation of HLA-antigens allele variants with clinical dysplasia variants and specific immune alterations. The findings support the suggestion that clinical heterogeneity of connective tissue dysplasia forms results from their pathogenetic heterogeneity.

Adolescent↗
PubMed1993

[Immunogenetic methods in the prognosis of the efficacy of using a method of transfusing extracorporeally irradiated autologous blood for treating patients with rheumatoid arthritis].

HLA antigens distribution among subgroups of rheumatoid arthritis (RA) patients was compared with reference to the results achieved after the treatment with transfusion of extracorporeally irradiated autologous blood (TEIB). The treatment efficacy was found to be in general 66% and to depend on HLA phenotype, age of the patients, the RA activity. The analysis of associations of clinical significance exhibited by clinical, laboratory and immunogenetic signs made it possible to derive a formula for individual prognostic criterion capable of raising TEIB efficacy to 89%.

Adult↗
PubMed1993

[Frequency distribution of HLA phenotypes, haplotypes and alleles in ethnic groups of Taĭmyr and Chukotka].

HLA typing data of two little populations living in Tajmyr peninsula (Dolgans and Nganasans) and Chukotka peninsula (Chukchas and Chuvantses) were presented. Our data were compared with the International standard distribution of class I HLA antigens in orients and its distribution in orients who live in the Asian part of Russia. Indexes of genetic distribution and genetic likeness were calculated.

Alleles↗
PubMed1993

[Features of the distribution of alleles of the HLA-DRB1 04 and HLA-DQB1 03 genes among healthy people of European origin in Western Siberia].

The allelic HLA-DRB1 04 and HLA-DQB1 03 polymorphism in caucasians living among the West Siberia Mongoloid aborigenes was studied. As a result of our studies, it was shown that the HLA-DRB1 0403/07 predominates and HLA-DRB1 0404/08 is absent in the Russian population of West Siberia, in contrast to those among Caucasians living in West Europe and North America. The frequencies of HLA-DQB1 03 alleles are similar to those observed among the all Caucasians. Gametic association HLA-DR4 - HLA-DQw was found for the first time in Caucasians of West Siberia.

Adult↗
PubMed1992

[Comparative analysis of indicators of the immune status of population of the Eastern regions of the USSR].

Altogether 1,500 healthy residents of seven cities situated in the Asian part of the USSR were examined. In Novosibirsk, Tomsk, Tyumen, Norilsk, Magadan, Yakutsk and Ussuriisk, the people were examined for the blood levels of T and B lymphocytes, the ratio of regulatory subclasses of T lymphocytes, the concentration of IgG, IgM and IgA, and for the content of immune complexes. Analysis was made of the general and regional regularities in changes seen in the immune system depending on climatic and geographic factors. Parameters of the populations similarity in the regions with different environmental conditions were delineated.

Adolescent↗
PubMed1992

[Evaluation of the effectiveness of various methods of the treatment of tick-borne encephalitis in the acute period].

To reveal the dependence of the efficacy of the treatment of tick-borne encephalitis (TBE) on the patient's HLA phenotype and to elaborate recommendations for individual treatment, 98 subjects who suffered TBE and who received different types of therapy in the acute period (isolated or combined use of specific homologous immunoglobulin and RNase) were subjected to immunogenetic examinations. The clinical follow-up and immunological examination of the subjects indicated made it possible to distinguish groups with a favorable and unfavourable outcome due to the treatment carried out in the acute period. Computer processing of the immunogenetic data on the patients with TBE allowed the deriving of individual predictors of the efficacy of the symptomatic and etiotropic therapy of TBE. The final effect due to the application of agents possessing an immunomodulating effect on specific humoral immune response is determined not only by the gravity of viral infection but is also associated with a number of allele variants of HLA complex genes which the patient has.

Acute Disease↗
PubMed1990

[Immunogenetic methods for predicting the clinical course of tick-borne encephalitis].

Distribution of HLA antigens, haplotypes and phenotypes of the histocompatibility complex was studied and compared in 110 patients with tick borne encephalitis, living in Novosibirsk. The disease patterns and varieties were many and varied. Distribution of HLA antigens in 140 healthy subjects also living in Novosibirsk served as control. Based on the results of the immunogenetic examination of patients with tick borne encephalitis, the methods were elaborated, enabling one to predict with a high accuracy of probability the development of the feverish, meningeal or focal forms of tick borne encephalitis, one- or two-wave disease variety within the first days of disease. Concurrent analysis of the sex of the person bitten by the virulent tick and combination in one genotype of several allelic forms of the main histocompatibility complex genes allows one to raise appreciably the prognostic significance of the immunogenetic examination and to elaborate high-informative individual diagnostic criteria.

Adolescent↗
PubMed1990

[A clinico-immunogenetic method of prognosis in retrobulbar neuritis as an initial manifestation of multiple sclerosis].

Examination of 166 patients with optic neuritis revealed that 65 of them developed multiple sclerosis (MS) at different times after optic neuritis. The observation period was 9.4 years on the average. Using the clinical, laboratory, immunogenetic methods the clinico-immunogenetic heterogeneity of optic neuritis was discovered, the highly informative specific clinical signs and immunogenetic markers suitable as criteria for predicting different variants of optic neuritis outcomes were defined. Using a heterogeneous successive procedure a method of individual ++pre-nosological prediction of MS in persons with a history of optic neuritis was devised.

Adolescent↗
PubMed1989

[Clinico-immunologic parallels in multiple sclerosis].

HLA antigens were compared in groups of patients with disseminated sclerosis and healthy donors. In remission group as compared with controls, increased were the rates of A9, B7, B13, B35, A0/B17, A1/B7, A9/B7, A3/B35, A11/B7, A11/B8, B5, B7, 15, and in the progredient group--B7, Bw22, B35, A1/B7, A2/B7, A2/B35, A9/B7, B7, 12. These groups were different only in A11/B7 rates. Remitting and progredient course of the disease in women differed in A3 and A10 rates, in men in A2 and A11. In men with remitting course the A2 antigen rate was substantially lower than in any other group. With respect to the disease duration and the degree of patients's invalidization, the groups of benign, malignant, and intermediate course were singled out differing from each other and controls also in their HLA composition.

Adolescent↗
PubMed1989

[Immunologic and immunogenetic heterogeneity of systemic and discoid lupus erythematosus].

The immune and endocrine systems and HLA genotype were subjected to a comparative study in patients with systemic and discoid lupus erythematosus (SLE, DLE). The patients suffering from these diseases were found to differ in a number of the parameters of the immune status including the content in blood serum and supernatant of the cultivated mononuclear cells of the soluble molecules HLA-A, HLA-B and HLA-DR. The degree of the SLE and DLE association with the genes and haplotypes of class I HLA complex was different as was the character of the association of HLA-A and HLA-B specificities with the activity of the immune system cells and with hydrocortisone content in plasma. The common immunogenetic syndrome characteristic of SLE and DLE patients has been identified.

Adolescent↗
PubMed1988

[Immunogenetic markers of disseminated sclerosis in western Siberia].

The contents of class 1 HLA antigens was compared in patients with disseminated sclerosis, their first-grade relatives and healthy donors. Patients, as compared to healthy persons, had higher occurrence rate of A1, A9, B7, A0/B0, A9/B7, A1/B7, A1/B12, A2/B7, B7, 27, B5, 0, B7, 12, B7, 15, B5, 7, and lower A28, B13, Bw22, B35, 40, B7, 35, A2, w19, A0/B35, A0/B13, A2/B15, A3/B35. In male patients, as compared to female patients, B40 and A1/B15 were more frequent, and B12 less frequent. The subgroup of patients with the diseases onset before 20 years considerably differed from other patients in their rates of A1, A1, 2, A1w19, A1/B0 increased and A3 and A9/B17 decreased. Compared to donor group, the patients' relatives had differences similar to those between patients and relatives. High relative risk values were characteristic of some of the above associations.

Adult↗
PubMed1988

[Study of the changes in the expression of histocompatibility antigens on the monocyte surface in the evaluation of the macrophage component of the immune system in rheumatoid arthritis].

Peripheral blood monocytes from 23 healthy persons and 25 RA patients were studied by cytohistochemistry, indirect immunofluorescence and cytophotometry to reveal interrelationship between cellular function of the system of mononuclear phagocytes and the expression of HLA-complex antigens in health and in RA. An increase in the phagocytic and metabolic activity of circulating monocytes was shown to be associated with a degree of the activity of an inflammatory process. A decrease in the level of the expression of HLA antigens class I was established in parallel with the rearrangement of the numerical ratio of cells expressing low and high levels of class II HLA-molecules. A degree of disorders of the expression of HLA-molecules and monocytic function was associated with a degree of RA activity.

Adolescent↗
PubMed1987

[Time dependence of the shedding of class I and II HLA antigens. Interrelation of the fluctuations of HLA-antigen shedding and 51Cr-labeled macromolecules].

Soluble HLA-A, -B and -DR antigens were determined every 10 min with a rate nephelometry method in supernatants of human mononuclear cells cultured for 3 hrs. The time-dependent oscillations in the quantity of soluble HLA antigens with ultradian (circadian) rhythm characteristics were found. Quantity oscillations of HLA-A antigens were synphase to HLA-B ones and antiphase to HLA-DR antigens. Amplitude characteristics were found to depend on the temperature and cytoskeleton activity.

Chromium Radioisotopes↗
PubMed1986

[Changes in the levels of soluble HLA antigens and their light chains (beta 2 microglobulin) in rheumatoid arthritis and systemic lupus erythematosus].

An increase in the level of soluble HLA-A and B antigens in the blood serum of patients with rheumatoid arthritis and systemic lupus erythematosus was observed using IKO-1 monoclonal antibodies and polyspecific alloantisera. This increase was accompanied by the elevation of the concentration of beta 2-microglobulin (the light chain of the molecules of HLA-antigens of the 1st class). A degree of the concentration of soluble HLA-antigens and beta 2-microglobulin depended on a degree of disease activity, a variant of a clinical course and the presence of visceral involvement.

Adolescent↗