PubMed Health⌕ Search

Biomedical subjects

V I Korinteli

Publications and source records attributed to V I Korinteli.

14 recordsLinked to original sources
PubMed1990

The virulent bacteriophage IRA of Salmonella typhimurium: cloning of phage genes which are potentially lethal for the host cell.

Morphological and biological properties of Salmonella typhimurium phage IRA were studied. The phage is a member of the Styloviridae family and exhibits a very wide spectrum of lytic activity. Molecular cloning of phage genes whose expression is lethal for the host cell has been performed and consequences of gene expression have been investigated. Expression of recombinant plasmid pKI71 causes structural changes of the cell wall and also degradation of host DNA while plasmid DNA remains intact. Expression of pKI72 blocks normal cell division. The possibility of applying such recombinant clones in marking pathways of microbial contamination in water areas is proposed.

Bacteriolysis↗
PubMed1989

[A study of the subcellular structure of the rabbit liver during the development of a bacterial infection].

Lysosomal, microsomal and peroxisomal fractions have been isolated from the liver cells of rabbits infected with Staphylococcus aureus pathogenic strain 274 by differential centrifugation in the density gradient of sucrose. The study of the fractions of cell organelles by electron-cytochemical and biochemical methods in the dynamics of the development of infection has revealed changes in the activity of marker enzymes, which is indicative of structural and functional shifts occurring in subcellular structures.

Animals↗
PubMed1987

[Morphological changes in the liver cells of rabbits with a bacterial infection].

In the process of bacterial infection in rabbits, their livers have been studied by means of light and electron microscopy, as well as by morphometric analysis. The results evidence pathomorphologic and ultrastructural changes occurring in liver cells. These changes, probably, cause different metabolic disturbances and thus facilitate the development of destructive processes in vital organs.

Animals↗
PubMed1987

[Changes in the functional activity of the liver cells in rabbits during a bacterial infection].

In the process of the development of bacterial infections, the activity of marker enzymes in the membrane structure of liver cells has been studied in rabbits by biochemical and electron-histochemical methods. The results of these studies have shown that, simultaneously with the progressing course of cell pathology and inhibition of the function of mitochondria, microsomes and peroxisomes, the lysosomes activate for the realization of the protective function formed in the course of evolution. At the same time the excessive labilization of lysosomal membranes facilitates the development of cell pathology, which is confirmed by the accumulation of residual bodies in the cytoplasm.

Animals↗
PubMed1985

[Role of lysosomes during the development of a fibroblast staphylococcal infection].

The study of staphylococcal infection in chick embryo fibroblasts, carried out by electron-histochemical and morphometric methods, has revealed the presence of correlation between the degree of fibroblast destruction and the permeability of lysosomal membranes. The development of bacterial infection, depending on the presence of homologous bacteriophages in the medium, has also been studied.

Animals↗
PubMed1984

[Fibroblast phosphatase activity in the staphylococcal infection process].

The study of the phosphatase activity of chick fibroblasts in the process of staphylococcal infection by the electron-histochemical method has revealed the presence of correlation between the degree of cytoplasmic destruction in fibroblasts and the level of acid phosphatase activity. Changes in the phosphatase activity of fibroblasts in the process of bacterial infection, depending on the presence of homologous bacteriophages in the system used as a model, have also been studied.

Acid Phosphatase↗
PubMed1979

[Electron microscopic study of experimental infection. II. A study of the dynamics of Shigella infection].

The dynamics of experimental Shigella infection of chick embryo fibroblasts was studied with the use of electron microscopy. The antibiotic-resistant forms of Sh. sonnei 1,188 was found to be incapable of invasion into the fibroblast cytoplasm and intracellular proliferation. The destruction of fibroblasts observed during the infection was seemingly caused by the action of bacterial endotoxins.

Animals↗