Biomedical subjects

V I Litvinov

Publications and source records attributed to V I Litvinov.

At least 19 recordsLinked to original sources

PubMed1991

[The characterization of the spectrum of the antibody response to Mycobacterium tuberculosis antigenic determinants by immunoblotting].

The spectrum of antibody response to M. tuberculosis antigenic determinants H37Rv and M. bovis antigenic determinants BCG was studied in serum samples from 33 healthy donors and 31 patients with infiltrative pulmonary tuberculosis by the method of immunoblotting. The study revealed that most frequently tuberculosis patients showed response to Ag-H37Rv with molecular weights of 52, 39, 35, 21, 31, 68 kD (44.4-22.2%) and Ag-BCG with molecular weights 60, 58, 50, 25, 54, 70 kD. (33.3-22.2%). By month 9 of effective chemotherapy binding predominantly with Ag-H37Rv determinants of 31, 62, 35, 75, 56, 28, 19, 5, 13 kD (75-37.5%) and Ag-BCG determinants of 13, 34, 38, 44, 19, 36, 45, 52, 58, 60 70 kD (37.5-25%) were registered. Some differences in the spectra of antibody response to Ag-H37Rv and Ag-BCG determinants were noted.

Antibodies, Bacterial↗

PubMed1991

[Anti-tuberculosis antibodies in the immunoenzyme test in relation to the effectiveness of chemotherapy of tuberculosis].

A method of indirect solid-phase EIA was used to detect serum antibodies in 110 patients with destructive tuberculosis. The preparation with a molecular mass of 38-42 kD isolated from the mycobacteria H37Rv was used as an antigen. Changes in the level of antituberculous antibodies were found during treatment, which are most manifested in 4-6 months of antituberculous therapy and depend on treatment efficiency. The effective therapy is accompanied by a decrease in the level of antituberculous antibodies by that time, while in ineffective therapy it remains high. Hence, changes in the level of antituberculous antibodies may serve as an additional criterion of chemotherapy efficiency.

Adolescent↗

PubMed1991

[Antituberculosis antibodies detected by an immunoenzyme test in patients with pulmonary tuberculosis].

The method of indirect solid-phase enzyme immunoassay (EIA) was used to detect antibodies in the sera of 166 pulmonary tuberculosis patients and 56 healthy donors. A preparation with a mol. mass of 38-42 kD was used as an antigen which was isolated from the mycobacteria H37Rv by a consecutive separation under high pressure, extraction of KCl cellular membranes and gel-filtration in the gel Toyopearl HW 55F. Antituberculous antibodies (AtAb) were detected by the EIA method in 94% of pulmonary tuberculosis patients which was much higher as compared to the same parameter in healthy subjects (10.7%). Hence, AtAb detection by this method can serve as an additional criterion for tuberculosis diagnosis. The detection rate and AtAb level are higher in fibrocavernous tuberculosis than those in infiltrative tuberculosis. The AtAb detection rate is higher in manifested intoxication than in moderate one or its absence. AtAb are more often detected in chronic than in newly diagnosed tuberculosis, in the disseminated forms than in the limited forms, in pronounced infiltration in the lungs as compared to a moderate form, and also in patients with bacillary excretion than in those whose sputum had no M. tuberculosis.

Adolescent↗

PubMed1991

[Genetic engineering production of H37Rv M. tuberculosis proteins].

A complete library of M. tuberculosis H37Rv genes was produced by incorporating the DNA fragments of M. tuberculosis H37Rv into the lambda pSI phasmid. For this, DNA isolated from the mycobacteria was treated by EcoRI restrictases and the fragments of 8-17 thousand nucleotide pairs were crosslinked with the phasmid DNA. Hybrid DNA molecules were packed into the caspids from the proteins of E. coli BHB2688 and BHB2690 strains. By estimates, this library contained 98% of M. tuberculosis H37Rv genome so that any required gene can be found at 0.99 probability. The needed genes were sought by monoclonal antibodies against a protein with a molecular mass of 17-19 kDa (IT-12, IT-51, IT-54) obtained from the WHO. The protein gene was also produced by the method for raising the end sequences using synthesis of two oligonucleotides SP30 complementary to segments that limit this gene in the presence of Taq-DNA-polymerase and DNA of M. tuberculosis H37Rv. Copies of a gene (MT-1) were produced by denaturation, firing and raising. This gene was incorporated in the puC119 plasmid and expressed in E. coli cells, the direction of reading being checked up. A protein with a molecular mass of 19 kDa was detected in E. coli extracts with the expressed pMT-2 plasmid using monoclonal antibodies IT-12 and IT-54 in enzyme-linked immunoassay and immunoblotting.

Antibodies, Monoclonal↗

PubMed1990

Tuberculosis in patients with various HLA phenotypes.

Tuberculosis patients and healthy subjects from six ethnic groups of the Soviet Union were HLA-A, -B, -C, and DR typed. The frequencies of the HLA-A, -B and -C antigens differed amongst the ethnic groups. With all groups, however, patients with tuberculosis showed a significantly increased frequency of HLA-DR2 and a reduced frequency of HLA-DR3 type. Unfavourable dynamics of tuberculosis was significantly associated with an increased incidence of B15 and DR2 and a reduced incidence of B27 and DR3. Family studies revealed that the inheritance of susceptibility to tuberculosis (from parent to offspring) is associated with the inheritance of certain HLA haplotypes. Tuberculosis patients bearing the DR2 antigen had increased levels of IgG antibodies to PPD and the frequency of B7 and, more particularly, DR2 was higher in anergic patients.

Antibodies, Bacterial↗

PubMed1990