PubMed Health⌕ Search

Biomedical subjects

V I Novikova

Publications and source records attributed to V I Novikova.

18 recordsLinked to original sources
PubMed1979

[Role of T- and B-lymphocytes in the heterogeneity of human cell-mediated reactions to bacterial antigens].

Leucocytes from 30 patients with allergy to tuberculin and bacterial antigens were treated with antithymus (ATS) and anti-immune globulin (AIGS) sera. The leucocyte migration inhibition test (LMIT) was performed with these antigens. ATS abolished the LMIT induced by tuberculin and sometimes by bacterial antigens (staphylococcal, streptococcal etc.). AIGS frequently abolished the LMIT induced by bacterial antigens, but not by tuberculin. In some cases the treatment with any serum abolished the LMIT induced by the antigens, or, on the contrary, it was abolished only by a successive treatment with both sera. The lymphocyte types (T or B) determining the secondary immune response to the same antigen are different in various patients, as well as they differ in the same patients in relation to diverse antigens. Five types of lymphocyte - antigen interrelation in the LMIT have been distinguished.

Adult↗
PubMed1978

[Detection of lymphokines in patients' blood serum].

A factor suppressing the migration of donor leukocytes and macrophages of guinea pigs in vitro was revealed in the blood serum of patients suffering from chronic inflammatory diseases (pneumonia, rheumatism, tuberculosis) and carcinoma. A factor stimulating the leukocyte migration was sometimes revealed in the blood sera of the patients. In chromatography of the blood sera on sephadex G-100 the activity of both factors proved to localize in fractions with the mol wt of 15000--45000 dalton. Depression of stimulation of leukocyte migration could be also caused by immunoglobulin fractions (mol wt--150000 dalton) of the blood sera of patients suffering from acute pneumonia, apparently on account of the presence in them of the antigen-antibody complex; however, these sera contained no migration suppression factor. The blood serum fractions with the mol wt of 15000--45000 dalton, including those containing the migration suppression factor inhibited the inhibited the spontaneous and induced by phytohemagglutinin blast transformation lymphocytes, and the immunoglobulin ones--the latter only. Apparently the migration suppression factor of the blood serum served as the product of activated lymphocytes.

Acute Disease↗
PubMed1976

[Determining hypersensitivity to antigens by computing cells migrating from capillary tubes].

The test technique of inhibition of leukocyte and macrophage migration from the capillaries in the presence of a specific antigen is described. The method is highly sensitive and permitted to use a small amount of cells and many (up to 600 and more) capillaries. Sodium azide, phitohemagglutinin and incubation at 4 degrees C inhibited the cell migration from the capillaries. Dead cells did not pass from the capillaries into the medium. Tuberculin and BCG vaccine inhibited the migration of macrophages of guinea pigs immunized with BCG, but not with staphylococcus allergen.

Animals↗