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Biomedical subjects

V I Ogarkov

Publications and source records attributed to V I Ogarkov.

At least 19 recordsLinked to original sources
PubMed1989

[The use of passive hemagglutination for determining the fibronectin-binding properties of microorganisms].

The authors present the data that evidence in favour of employing fibronectin sensitized formalin-treated red cells for the indication of fibronectin-binding microorganisms (as exemplified by staphylococci) in the passive hemagglutination test (PHAT). The possibility of a quantitative estimation of fibronectin binding by the PHAT is shown. PHAT will help detect higher numbers of fibronectin-binding staphylococci than flocculation on the glass.

Fibronectins↗
PubMed1989

[An evaluation of the degree of serological affinity of the surface structures in Candida maltosa and Candida albicans].

The complex preparation of surface antigens was obtained by the treatment of C. maltosa whole cells with beta-mercaptoethanol and their separation into 8 fractions by means of ion exchange chromatography on DEAE cellulose. The sensitizing capacity of these fractions was studied in the allergic dermal test on guinea pigs and their immunochemical activity, in the immunodiffusion test with homologous antiserum and with the gamma-globulin fraction of antiserum to C. albicans. All fractions induced delayed hypersensitivity, more or less intensive, in guinea pigs. The agar immunodiffusion test revealed that the complex preparation contained two groups of fractions differing in their antigenic composition. Fractions of group 1 reacted equally well with homologous and heterologous antisera. Fractions of group 2, eluting at NaCl concentrations from 0.1M to 0.4M and having very high precipitation activity in reactions with homologous antiserum, showed considerably lower capacity for reaction with antiserum to C. albicans, which suggested that they contained antigenic structures differing from the antigenic determinants of C. albicans and thus ensuring specific reactions in cases of candidal sensitization induced by C. maltosa.

Animals↗
PubMed1988

[Use of the coagglutination reaction of yeast-like Candida maltosa fungi for detecting fimbriae in intestinal bacteria].

The capacity of nonpathogenic yeast-like C. maltosa strains to coagglutinate Escherichia coli has been studied. C. maltosa cells have also been shown to coagglutinate E. coli possessing mannose-sensitive adhesins in a wide range of their concentrations (5-140 bacterial cells per C. maltosa cell). Strains belonging to types CFA/I and CFA/II with fimbriae, similarly to their corresponding paired genetically related strains without these adhesins, are practically incapable of agglutinating C. maltosa cells, while strains K88 and B41 react with them. The reaction occurs at a concentration of 9.5-37.0 and 38.0-55.5 bacteria respectively per C. maltosa cell and is not inhibited by 1% d-mannose. The suggestion that C. maltosa cell surface glycoproteins contain not only receptors for E. coli fimbriae, type I, but also components similar in their structure to receptors specific to the mannose-resistant adhesins of strains K88, K99 and 41, has been confirmed by hemagglutination inhibition with C. maltosa surface antigens as inhibiting agents.

Adhesins, Escherichia coli↗
PubMed1988

[Fibronectin-binding capacity of staphylococci].

The fibronectin-binding capacity of clinical isolates of the genus Staphylococcus has been studied in the flake-formation test on glass and by inoculation into agar with human fibronectin added. Most of 58 S. aureus strains have been found capable of binding fibronectin. None of coagulase-negative staphylococci has given flake formation with fibronectin. The possibilities of the quantitative evaluation of the fibronectin-binding by the above-mentioned methods have been shown. In the analysis of the monomers of bovine fibronectin its capacity for inducing the growth of compact colonies in semiliquid agar has been shown.

Animals↗
PubMed1988

[Effect of anabol on the functional activity indices of mononuclear phagocytes].

In this investigation the influence of anabol, a biopolymer preparation incorporated into a complex of extracted components obtained from the surface layer of Lactobacillus bulgaricus cell wall, on some functional activity characteristics of mononuclear phagocytes has been studied. The injection of the preparation in a dose of 20 mg/kg of body weight 24 hours prior to the experiment leads to a decrease in the time necessary for the elimination of one-half of the total amount of ink from the blood stream and to an increase in the content of fibronectin in the blood plasma of rats. The activation of the metabolic activity of resident and induced macrophages, evaluated by the nitro blue tetrazolium reduction test, is observed. On the next day after the injection of anabol the sharp increase of the pool of granulocyte and microphage precursors is observed; the stimulating effect may be retained as long as 3 days. The results thus obtained indicate that anabol acts on different elements of the system of mononuclear phagocytes and can be effective in the complex therapy aimed at the increase of the body resistance to any toxic substances and bacterial infection.

Animals↗
PubMed1988

[Effect of anabol on the functional activity indices of mononuclear phagocytes].

Anabol is a biopolymer from the complex of components extracted from the surface layer of the Lactobacillus bulgaricus cell wall. Its effect on certain indices of the functional activity of mononuclear phagocytes was studied. Administration of the preparation in a dose of 20 mg/kg 24 hours before the investigation resulted in lowering of the stain half-life in the general blood flow and increasing of the fibronectin levels in blood plasma of rats. Activation of the metabolic activity estimated by nitroblue tetrazolium test as well as resident cells and induced macrophages was observed. 24 hours after the anabol administration there was noted a marked increase in the pool of the precursors of granulocytes and macrophages, the stimulating effect being preserved for up to 3 days. The results showed that anabol had an effect on various elements of the system of mononuclear phagocytes. It may be useful in complex therapy aimed at increasing the host resistance to diverse toxic substances and bacterial infection.

Animals↗
PubMed1988

[Induction of immunologic deficiency by benzene and its correction by administration of anabol].

Cellular immunity and activity of enzymes of xenobiotic metabolism have been investigated. It has been shown that administration of benzene induces a stable immune deficiency syndrome characterized by a decrease in the quantity if antibody-forming cells, T-killers and T-suppressors. The activity of enzymes (cytochrome P-450 and cytochrome C reductase) was also inhibited. It has been shown that anabol can stimulate the parameters of cellular immunity and enzyme activity. Benzene intoxication was demonstrated to be a model of immune deficiency syndrome similar to the clinical pattern. Anabol was shown to be an effective immunomodulator.

Adjuvants, Immunologic↗
PubMed1987

[Cellular immunity and enzyme activity of xenobiotic metabolism in the lymphocytes of normal minipigs and in alcohol and anabol exposures].

Immunological indices and activity of xenobiotic metabolism enzymes in lymphocytes were studied on minipigs under normal conditions, under conditions of chronic alcoholic intoxication and after administration of anabol (an immunomodulator) to normal healthy animals and to animals with alcohol intoxication. Age-related differences with respect to the number of T-lymphocytes and activity of lymphocyte glutathione S-transferase were observed in the normal animals, the other indices such as activity of natural killer cells, K-cells, blast cell transformation with concanavalin A and activity of cytochrome c-reductase being independent of the age. Administration of anabol to healthy animals did not alter their immunoenzymatic status. Chronic alcohol intoxication was accompanied by development of secondary immune deficiency characterized by lower immunological indices and lower activity of xenobiotic metabolism enzymes in lymphocytes. Daily exposure to 0.8 g of anabol for 12 days at this background resulted in normalization of the above indices.

Adjuvants, Immunologic↗
PubMed1987

[Effect of blastolysin on the ultrastructure and functional activity of mast cells and eosinophils].

Blastolysin is shown to cause degranulation of mast cells, resulting in a reduction of their histamine content and in their release of mediators that activate eosinophilic leukocytes (EL). These accelerate the deamination of the histamine released by mast cells to the extracellular medium. It is possible that blastolysin acts directly on EL granules which are largely responsible for histamine inactivation.

Animals↗
PubMed1986

[Standardization of the direct hemagglutination reaction induced by bacteria of the genus Lactobacillus].

L. bulgaricus, L. casei var. rhamnosus, and L. plantarum cells grown in agar-containing (but not liquid) thioglycol medium for 3 days have been shown to be capable of inducing mannose-resistant agglutination of human and guinea-pig erythrocytes, while giving a considerably weaker reaction with erythrocytes from animals of other species (ox, sheep, dog, rabbit, rat and mouse). The optimum conditions for the test are presented.

Animals↗
PubMed1986

[Characteristics of blastolysin preparations in their ability to inhibit hemagglutination caused by bacteria of the genus Lactobacillus].

Anabol and blastolysin preparations obtained from L. bulgaricus may contain surface structural components of the initial strain with adhesion activity; of these, one is similar in specificity to L. casei adhesin and the other, to L. plantarum adhesin. The antigenic activity of anabol and blastolysin, evaluated in the immunodiffusion test, does not correlate with their capacity for binding the receptors of susceptible bacterial cells, determined in the Lactobacillus-induced hemagglutination inhibition test.

Anti-Bacterial Agents↗
PubMed1985

[Immunochemical study of blastolysin].

The immunochemical properties of blastolysin (BL), a preparation obtained from the cell walls of Lactobacillus bulgaricus with immunostimulating and antitumor activity were studied. The experiments on dogs and rabbits showed that the immunogenic effect of BL was not high. However, after parenteral administration to the dogs and rabbits it induced production of the specific antibodies detected with the Ouchterlony test of direct hemagglutination and immunodiffusion. This should be taken into account in development of the schemes for its use as a pharmacological agent. With the procedures of gel filtration on the columns with Sephadex G-75 and immunodiffusion the fraction composition of BL was determined and the use of the immunochemical procedures for standardization of the BL preparations was shown to be possible.

Animals↗
PubMed1985

[Effect of blastolysin on the ultrastructure and phagocytic activity of peritoneal macrophages].

Morphology and functional activity of peritoneal macrophages were studied at different intervals after the administration of blastolysin to Wistar rats. Alteration of the macrophage ultrastructure and their functional activity proving the stimulatory effect of the preparation on cells is shown. The signs of stimulation were revealed 30 minutes after the administration of the drug, reached a maximum on the 7th and 14th days and was manifested by structural changes of the cell surface, lysosomal apparatus, mitochondria and by activation of phagocytosis.

Adjuvants, Immunologic↗