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V I Romanenko

Publications and source records attributed to V I Romanenko.

31 records · Page 2Linked to original sources
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[Development of bacteria and their activity in an aqueous surface film under experimental conditions].

The formation and number of bacteria in the surface film of water depend on the content of organic substances in water. In flasks containing distilled water after 1-2 days, a bacterial film 5-7 mcm thick is formed at the surface; in flasks with water taken from reservoirs, the film is 5-15 mcm thick; in flasks with water containing organic substances, the film is 15-50 mcm thick. A distinct bacterial band of growth is formed on glasses semisubmerged in water in this layer. At the beginning, bacterial rodshaped cells are oriented by their side surface to the surface layer of water. Judging by the assimilation of protein labeled hydrolysate, the total activity of bacterial population in a film is much higher than in deep water layers. In the course of time, the number of bacteria changes, they begin to die off, and their species are substituted by other bacterial species. Numerous motile forms and various Caulobacterial forms are found in the surface layer of water. The dimensions of bacterial cells growing on glass are much larger than in dry preparations obtained from this film on membrane filters.

Bacteria↗
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[Specific gravity of the dried biomass of pure bacterial cultures].

The weight of wet and dry biomass taken from colonies after growth on MPA was determined in pure bacterial cultures of Pseudomonas denitrificans and Brevibacterium imperiale, and the number and dimensions of bacterial cells in dry preparations of the same colonies were measured by means of membrane filters. The average specific weight of dry biomass was found to be 1.68 and 1.62. In calculating the weight of bacterial biomass in pure cultures and natural cenoses, its volume should be assayed taking into account the number and dimensions of the cells in dry preparations on membrane filters and multiplying by a coefficient 1.6.

Bacteria↗
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[Bacterial destruction of algal organic matter with glucose formation].

The increase in the number of bacteria, the rate of destruction of organic matter, and the rate of glucose assimilation with analysis according to Wright and Hobbie were determined during 205 days in two bottles containing water from the Rybinsk Reservoir; one of the bottles contained also 25 mg of dry algal cells per one litre. During this period in the control, 39% of the initial amount of the organic matter was destructed (12 mg C per litre), and 4.6% of glucose was formed from this quantity. In the experimental series, 85.6% of the initial amount of the organic matter underwent destruction (from 12.5 mg C per litre), and 48.5% of glucose was formed from this amount.

Bacteria↗
PubMedDate not supplied

[Pure culture of bacteria using chromates and bichromates as hydrogen acceptors during development under anaerobic conditions].

A pure bacterial culture utilizing chromates and bichromates as oxygen donors during growth on organic substances in anaerobic conditions was isolated from active ooze of sewage and industrial wastes containing a weak solution of chromates. A medium for growth of the bacterium was selected, and its morphological and cultural properties were studied. Chromates and bichromates are reduced only in anaerobic conditions by the bacterium or active ooze containing it, the hexavalent chromium becoming trivalent. The culture does not belong to any known species and has been therefore classed as Pseudomonas dechromaticans.

Anaerobiosis↗