[Study of the possibility of obtaining Pseudomonas vignae cell walls under the influence of ultrasonics and the adsorption on them of bacteriophage].
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Biomedical subjects
Publications and source records attributed to V I Ruban.
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Electron microscopic examinations showed the moderate phage 59 Erwinia carotovora to consist of a head of icosahedral shape 50.0+/- +/-2.5 nm in diameter, a noncontractile process 150.0+/-3.5 nm in length and 8.2+/-1.0 nm in diameter with a basal plate of 20.0+/-1,5 nm. The head of the virion of 45.9 X 10(3) nm3 in volume is formed by 812 morphological subunits about 3.0 nm in diameter; the number of structural subunits is 4860 on the basis of triangulation number (81). The process of the virus is cross-striated, the pattern being formed by 35+/-1 rows (43+/-5 A) of subunits 3.0 nm in diameter. The total number of subunits in the process is 210+/-6. DNA of the virus under study has a trend of ring formation. Its modal length is 14.61+/-0.53 micron corresponding to a molecular weight of 28.66+/-1.04 X 10(6) daltons. The volume of DNA molecule is 45.9 X X 10(3) nm3, indicating that a portion of phage DNA is in the virion, probably in denaturated state. An electron microscopic model of the organization of the virus under study is proposed.
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The method of electron microscopy has been used to study adhesion of the microbic cells of standard strains of Staphylococcus aureus, Escherichia coli and fungi of genus Candida on the organosilicon sorbent polymethylsiloxane (PMS) and medicamentous complex containing it. This complex contains furazolidone and metronidazole immobilized on silver ions-modified PMS. It is shown that the adhesion of microorganisms is accompanied by their destruction whose rate on pure PMS and medicamentous complex is different. Using experimental data the assumptions are advanced concerning the mechanism of the PMS interaction with Gram-positive and Gram-negative microorganisms as well as with fungi of genus Candida.
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