Biomedical subjects

V I Zlobin

Publications and source records attributed to V I Zlobin.

25 records · Page 2Linked to original sources

PubMedDate not supplied

[The use of molecular hybridization with synthetic deoxyoligonucleotides for differentiating strains of the tick-borne encephalitis virus].

Synthetic deoxyoligonucleotides complementary to different regions of genome RNA of tick-borne encephalitis (TBE) Sofjin virus strain were used to differentiate TBE virus strains. Nine TBE strains isolated in different geographical areas from different sources and several viruses of the TBE subgroup were tested. The probes revealed genetic heterogeneity of TBE strains. The probes complementary to different regions of the genome had different specificity. The pattern of hybridization of TBE virus strains with a panel of 11 oligonucleotide probes correlated significantly with the source of the virus strain and to a smaller extent with the geographical isolation site.

Animals↗

PubMedDate not supplied

[Comparison of 3 express-methods of detection of tick-borne encephalitis virus].

Comparative studies of the diagnostic value of three express methods for detection of tick-borne encephalitis virus in ticks (fluorescent antibody technique (FAT) enzyme immunoassay (EIA), and molecular hybridization of nucleic acids) and the traditional method (bioassay in white mice) showed all the three express methods to be rapid, specific, sensitive, and useful for large-scale epidemiological surveys. Notable was the high effectiveness of the method of nucleic acids hybridization which was not inferior to bioassays in suckling mice and exceeded FAT and EIA. The results of the latter seem to be affected by antigenic variations among tick-borne encephalitis virus strains.

Animals↗

PubMedDate not supplied

[Genomic changes in strains of the tick-borne encephalitis virus as a result of passages in mice].

Changes in genomes of TBE strains isolated from various sources at early stages of laboratory adaptation to white mice brain were demonstrated by molecular hybridization of nucleic acids with synthetic oligonucleotide probes, complementary RNA sites of reference TBE strains Sofyin and Neudorf. Of the 4 TBE strains passed 6 times through white mice brain in only 1 the level of RNA hybridization with 2 oligonucleotide probes was found changed, whereas in all the 3 tested strains the level of RNA hybridization with 1-2 oligonucleotide probes complementary to strain Sofyin was increased after 7-16 passages in the same system. The most noticeable changes in the genome were detected during readaptation to white mice of a TBE variant selected in passages on H. marginatum marginatum ticks.

Adaptation, Physiological↗

PubMedDate not supplied

[The electrophoretic mobility of the virus-specific proteins of tick-borne encephalitis virus strains isolated in different geographical regions of the CIS Nations].

Tick-borne encephalitis (TBE) virus strains isolated in different geographic areas were characterized by changes in electrophoretic mobility (EPM) of virus-specific proteins. Analysis of EPM of intracellular virus-specific protein E and other high molecular proteins of 42 TBE virus strains revealed similarity among the majority of them, with the exception of several strains including the Sophyin strain (Far-Eastern subtype). The most marked variability in the electrophoretic behavior of virus-specific proteins was observed with low molecular proteins prM, ns4b, ns2a.

Animals↗

PubMedDate not supplied

[The genetic characteristics of the Vergina serotype of the tick-borne encephalitis virus and its pathogenetic traits].

The Greek Vergina strain of tick-borne encephalitis (TBE) virus was studied in comparison with 7 other strains by molecular hybridization of nucleic acids and by clinicomorphological markers of pathogenicity for monkeys and Syrian hamsters. By the genetical features the Vergina strain differed from the eastern and western TBE subtypes but was found to be similar to the strains of other subtypes of the Urals-Siberian, east-Siberian (Aina-1448) and Central Asian antigenic variant. This group of strains hybridized with cDNA at 65 degrees C only in the absence of 50% formamide, reacted with probe 1115 complementary to protein E gene, with 1-3 probes complementary to the conservative region of the genome but did not react with the probes corresponding to the variable regions of the genome. The Vergina strain is close to TBE genotype III. The Vergina strain was found to be virulent inducing subacute meningoencephalomyelitis which developed slowly and was accompanied by less marked morphological lesions in the cerebral cortex than those induced by the eastern subtype. The Vergina strain was demonstrated to persist in the brain, liver, spleen, and lymph node tissues.

Animals↗

PubMedDate not supplied

[Vergina-like strains of tick-borne encephalitis in Russia].

The Greek strain Vergina representing an individual third serotype of tick-borne encephalitis (TBE) virus has been compared with 13 TBE strains isolated on the territory of Russia and Central Asia (in Kirghizia). A kit of deoxyoligonucleotide probes complementary to genome sites of Neudorfle strain of the TBE Central European subtype (protein C and prM genes) and of strain Sofyin of the Eastern subtype (protein E, C, M, prM, ns 1, ns 2b, ns 4b genes) was used in molecular hybridization of nucleic acids. Vergina strain was referred to the genetic variant VI prevalent in the western part of the East European plain, in Udmurtia, in the Altai mountains, and in West Siberia. By its antigenic properties Vergina strain is most close to strain Yar-90 isolated in the Yaroslavl district from Ixodes persulcatus ticks.

Animals↗

PubMedDate not supplied

[Possibility of occurrence of mild spinal forms of poliomyelitis in subjects immunized repeatedly with live poliomyelitis vaccine].

The results of clinical, epidemiological, serological and virological examinations of patients with mild spinal forms of poliomyelitis occurring under conditions of mass immunization with oral live poliomyelitis vaccine are presented. The etiological role of poliomyelitis virus types I and III in these diseases has been established. It has been shown that multiple vaccination does not always prevent diseases but in these cases they are milder. Serological examination of patients and normal subjects suggests inadequacy of seroconversion to poliomyelitis viruses under conditions of mass vaccination of the population against poliomyelitis.

Child, Preschool↗