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Biomedical subjects

V Ia Karmysheva

Publications and source records attributed to V Ia Karmysheva.

At least 19 recordsLinked to original sources
PubMed2003

[Cytopathological changes in human and animal brain in prion diseases].

The study of brain histological sections of humans and animals afflicted by prion diseases has shown that neuronal vacuolization began in and intensely spread from the distal portions of dendrites. Two types of neuronal cell body death were demonstrated, including cytolysis and pyknosis, the latter being usually associated with dendrite vacuolization. The involvement of major glial types in the pathogenesis of prion diseases was established, which argues against the concept of astrocytosis in favor glyosis. The accumulation of phagocytes (microgliocytes, blood macrophages, pericytes) and emigration of lymphocytes from the blood vessels with diffuse infiltration of brain tissue and formation of aggregates around degenerating neurons, were noted.

Animals↗
PubMed1992

[The morphology of cell lines suitable for vaccine production studied by scanning electron microscopy].

Five nontumorogenic cell lines suitable for vaccine production were studied by SEM. It was shown that diploid cell line 41 originated from sheep embryo kidney and also two heteroploid cell lines, line 4921 originated from embryo skin and muscles of the African green young monkey and line 4647 from kidney of the adult monkey, maintained normal cell morphology and normal growth pattern in early and in later passages in cultures. Some alterations in epithelial dense monolayer formation were revealed in the heteroploid cell lines: in line 455 originated from spleen of the adult African green monkey, and in line 4184 originated from line 41. The revealed alterations can be considered as the early morphological signs of the transformation of epithelial cells in culture. These cell lines also retained the stability of their morphological characteristics at the earlier and later passages. All the studied cell lines were free of contaminating agents.

Animals↗
PubMed1987

[Pathogenesis of persistent and chronic forms of tick-borne encephalitis (experimental study)].

A long-term experiment was conducted to study various aspects of the pathogenesis of persistent and chronic tick-borne encephalitis (TBE). Virological, serological, pathomorphological, electron microscopic and immunofluorescent techniques have been utilized in this study. Persistent TBE infection of Syrian hamsters examined over the period from 40 days to 2 years was characterized by the presence of virus-specific antigens in the organs and of specific antibodies in the blood serum. The persisting TBE virus was found to be predominantly localized in the central nervous system and spleen. Nerve cells underwent ultrastructural changes which were characteristic of flavivirus infection and related to the morphogenesis of viral particles. The authors have developed an experimental model of a primary progressive form of TBE with early and late manifestations of clinical symptoms of the disease.

Animals↗
PubMed1986

[Characteristics of human-mouse hybrid cell lines and the changes induced in them by the poliomyelitis virus].

Cells of the four hybrid lines between continuous mouse cells Rag and human diploid embryonal fibroblasts were polymorphic and had mitotic activity in fully formed monolayers. Most of the these mitoses were pathological. Hybrid cells examined 8 months after hybridization were susceptible to the poliomyelitis virus infection with partial cytopathologic effect, they produced virus antigens and the infectious virus. Small hybrid cells displayed a more pronounced cytopathologic effect than did big, polynuclear and mitotic cells. Hybrid cells that were passaged 1.5 months after infection did not excrete any infectious poliovirus but contained poliovirus antigens.

Animals↗
PubMed1985

[Cytotoxic activity of the serum of schizophrenic patients].

The action of blood serum obtained from 38 schizophrenic patients and 28 control subjects was studied on new cells: Chinese hamster kidney, diploid human cells and glial cultures of immature ependymoblastoma of type 5 mice (IE-5) and neurinoma of rat trigeminal ganglion. Sera of schizophrenic patients--especially of those with a continuous course and disease standing over 5 years and with a recurrent course and disease standing less than 5 years--were found to be significantly more toxic. In the culture of IU-5, sera from schizophrenic patients of a younger age (the mean age 27 years) exerted the most pronounced cytolytic action. Toxic activity of the serum of schizophrenic patients persisted for 14 days at +4 degrees C and 60 days at -30 degrees C but was reduced following thermal treatment (+56 degrees C, 30 min).

Adolescent↗
PubMed1983

[Serous meningitis in patients with herpes zoster].

CSF was studied in 110 patients with herpes zoster. The whole complex of symptoms helped to diagnose serous meningitis in 62% of cases and so-called "asymptomatic meningitis" in 18% of cases. CSF cells were characterized by lymphocyte transformation and the presence of antigen to Varicella zoster virus determined by the fluorescent antibody method. Specific antibodies titer appeared to be at zero or minimal level. The disease pathogenesis is discussed.

Adolescent↗
PubMed1980

[Inhibitory effect of homologous antiviral antibodies on viral cell destruction].

Experiments were performed on the model Chinese hamster cell culture -- Sindbis virus in which there develops an acute infection accompanied by active virus reproduction, cell rounding and detachment from the glass for 24--48 h. Addition in the cultural medium of a 0.5% immune serum to the Sindbis virus after its adsorption by the monolayer culture fully prevents the development of cytopathic changes and favours obtaining chronically infected cultures that produce the infection virus. The decreased production of intracellular and surface viral antigens in the infected cells seems likely to be accounted for by blocking the function of viral genome by homologous antibodies that join virus-induced antigens in the cellular plasmalemma.

Animals↗
PubMed1978

[Determination of the quality of fluorescent antibodies against human globulins using an antinuclear antibody standard].

The authors present data concerning the standardization of the assessment of fluorescent antibodies against human globulins. Fluorescent emision proved to depend on the biochemical indices of the conjugates and on the quality of the initial raw material of crude serum. Materials of quantitative recording of the strength of fluorescence of the nuclear antigen treated with fluorescent antibodies are presented. A difference between the activity and the specific activity (with respect to normal human serum) of the preparations was noted.

Animals↗
PubMed1977

[Preparation of a standard for antinuclear antibodies].

The authors prepared a laboratory standard of antinuclear antibodies from the serum pool of patients suffering from systemic lupus erythematosis. This standard was tested in respect to normal human serum and an international standard of antinuclear antibodies BRS ANA 66/233. A possibility of quantitative determination of the usefulness of the sera of patients suffering from systemic lupus erythematosis for the preparation of the standard was demonstrated.

Animals↗