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V Ia Shevliagin

Publications and source records attributed to V Ia Shevliagin.

At least 19 recordsLinked to original sources
PubMed1990

[The isolation and characteristics of long-term continuous lymphoid cell lines (IK-10 and IK-12) from patients with mycosis fungoides].

Examinations of 26 cell cultures have detected in two cases continuous IK-10 and IK-12 cell lines from patients with mycosis fungoides. Studies of these cell ultrastructure, carried out by transmission electron microscopy, have shown organoids of all types, that are characteristic of both minor and medium lymphocytes and of lymphoblasts. Markers of T-, B-cells and mononuclears are also detectable in these cell lines. The cells express surface immunoglobulins and interleukin-2 receptors. Noteworthy that association of these cells in a population may be in vitro cultivated for more than 2 years.

Cell Line↗
PubMed1990

[A clinico-experimental study of patients with mycosis fungoides and Kaposi's sarcoma].

Twenty-six cultures were prepared by the cultivation method. Continuous cells lines (IK-10 and IK-12) were obtained in two cases, in the rest short-lived cell cultures were prepared. All the isolated cell cultures were studied by transmission electron microscopy. Abnormal lymphoblasts were detected in some cell cultures isolated from the blood and lymph nodes of mycosis fungoides (IJ-137, IK-140, IK-142, IK-143) and Kaposi's sarcoma patients (IK-145). Cell population consisted of small, medium, and large lymphocytes. Electron microscopic examination has revealed retroviruses in IK-143 culture, isolated from the lymph node of a patient with mycosis fungoides. Morphologically these particles were typical of type C oncoviruses, 120-140 nm in diameter, with a spherical core in the center of the virion.

Adolescent↗
PubMed1990

[Effects of organic fluorosilicone compounds on the development of tumors in mice].

The influence of new synthesized fluoro-silicium-organic complexes on the virus-induced Rauscher leukosis and cell-transferred MX-11 mouse sarcoma was studied. We also studied the cytotoxic effects of these complexes in vitro in the human CaOv cells. Two complexes from seven studied were cytotoxic for CaOv cells. Five complexes from seven studied diminished the mortality of animals with MX-11 tumors on the 27-th day of observation, but the total life duration of the animals in the experimental group was the same as in controls. One complex from seven studied increased the life duration of mice with MX-11 tumors. No effects were noted in relation to mice virus-induced Rauscher leukosis.

Animals↗
PubMed1989

[The effect of gram-negative bacteria lipopolysaccharides on the development of Rauscher leukosis in BALB/c mice].

The dose-dependent action of Shigella sonnei lipopolysaccharide (LPS) on the development of acute erythroleukocytosis, as well as Rauscher chronic myeloid and lymphoid leukosis, in BALB/c mice sensitive to Rauscher virus was shown. Bordetella pertussis LPS in the doses used in this investigation stimulated the development of both acute erythroleukosis and chronic myeloid and lymphoid leukosis in BALB/c mice infected with Rauscher virus. Lipid A isolated from B. pertussis LPS was found to produce a stimulating effect on the development of Rauscher leukosis in mice. After the treatment of B. pertussis LPS with polymyxin B blocking lipid A no stimulating effect of B. pertussis LPS on the development of Rauscher leukosis was observed. A suggestion is made that lipid A is the active principle contributing to the stimulation of the development of Rauscher leukosis in BALB/c mice.

Animals↗
PubMed1989

[The coleukemogenic action of Bordetella pertussis].

Injection of B. pertussis and Rauscher leukemia virus (RLV) in a dose of 4 ID50 to BALB/c mice susceptible to the above virus significantly increases the incidence of leukosis and shortens the average life duration. Injection of B. pertussis to the AKR mice, carriers of the Gross leukosis virus, induces in the first months a greater number of the mice with leukosis and its earlier development.

Animals↗
PubMed1988

[Cloning of terminal fragments of the avian adenovirus CELO genome and isolation of a recombinant plasmid carrying the viral oncogene].

The terminal fragment of avian adenovirus CELO has been cloned in a plasmid vector. The obtained recombinant plasmid pCBE1 carries the terminal BamHI-E fragment of CELO DNA. Transfection of a nonpermissive culture of Rat2 cell line by the plasmid DNA results in formation of transformation focuses. The cloned BamHI-E fragment of CELO DNA is concluded to contain the viral oncogene. Thus, the CELO genome region deriving the BamHI-E fragment is "left".

Adenoviridae↗
PubMed1987

[Release of polyoma virus from transformed human cells].

Virus reproduction and activation were studied in human cells transformed with human polyoma virus. At the stage of spontaneous viral production, such methods of activation as somatic hybridization and DNA transfection were successful. Special methods of viral activation (treatment with mitomycin C and DEAE--dextran, and subsequent homologous DNA transfection) were required when viral production by cells ceased completely.

Cell Line↗
PubMed1986

[Bilayer agar cell cultures for studying cellular growth factors].

The method for biological testing of growth factors (GF) produced by transformed cells is described. The method is suitable for studying the ability of donor cells to release GF that stimulate colony formation of test-cells. Donor cells and test-cells are placed into different semisolid agar layers and separated by intermediate agar layer. The method provides a much more efficient testing of GF biological activity than the use of conditioned cultural fluids of transformed cells. It permits the assessment of GF dialyzation and the role of donor cell proliferation in the production of GF.

Animals↗
PubMed1985

[Genome expression of the DNA-containing human papovavirus in transformed human cells].

In the course of study of the transformed cells of line 63 the phenomenon of cyclically repeated transitory infection of the level of separate cells accompanied by the periodically isolated DNA-containing virus has been shown. Virus reproduction was judged by the determination of the infectious and hemagglutinating activities and radioactivity. The application of activation methods (co-culturing, somatic hybridization and cells treatment with mytomycin C) led to the increase of virus synthesis in the transformed cells of line 63 during spontaneous production of it. We failed to express viral genome in refractor phase.

Cell Transformation, Viral↗
PubMed1985

[Characteristics of a cell line obtained from a human hypernephroma].

A stable tumorigenic cell line has been obtained from the human hypernephrome. The culture grew as monolayers of large polygonal cells with foamy cytoplasm and 1-2 nucleoli. The cells formed monolayer after the third day after planting into flask and then formed multilayered growth. The electron microscopic studies of hypernephrome sections revealed the dark and light cells. The hypernephrome cells have characteristics of tumor cells. On the 200th passage the cells are aneuploid with a model number of 62 chromosomes; an acrocentric chromosome in D group is noted as a marker one. The hypernephrome cells are free of Mycoplasma contamination, able to support the several virus replication.

Animals↗
PubMed1984

[Pathogenic properties of the human papovavirus in the golden hamster model].

Human papovavirus isolated from human malignant paraganglioma was found to have pathologic relevance to golden hamsters of varying age inducing sarcomas in adults, when given intracerebrally, and lesions of central nervous system in newborns inoculated subcutaneously. T-antigen, immunologically related to that of SV40 was detected in tumor cells. Infectious papovavirus, identical to human one, was isolated from brain tissues of sick hamsters.

Animals↗
PubMed1979

[Relationship between Rous sarcoma virus production in golden hamster cells and the cell cycle].

The axon terminals of neurosecretory cells, containing elementary granules of neurosecretory materials, have been described on the basis of light and electron microscopy. No allochthonous neurosecretory elements were found in the sinus gland. The discharge of the granules from some terminals of the sinus gland occur with the changed salinity of the environment. There is a great difference in the structure of the sinus gland when salinity is changed. The structure of the sinus gland and its modification under experimental conditions indicate a possibility of neurohormonal regulation of the hydromineral balance.

Animals↗
PubMed1979

[Effect of mouse antiserum against isologous aggregated immunoglobulins on the transplantation and antitumor immunity].

It was shown that mouse antiserum against isologous thermically aggregated immunoglobulins MAAS injected repeatedly to the animals prolonged the survival of the skin allotransplant, induced Moloney's sarcoma diminished the latent period of the tumour development sharply increased the formation of the tumours which in the majority of cases, led to the death of mice, deranged the normal age barrier in fibrosarcoma induction, and sharply inhibited the intensity of Rauscher's leukemia development. This action of MAAS on the transplantation and antitumour immunity was of immunological nature.

Animals↗
PubMed1978

[Alterations of Rous virus and polyoma virus during their synthesis in the cells of unnatural hosts].

The line of human embryo fibroblasts transformed by Rous sarcoma virus (strain Schmidt-Ruppin) contained and produced Rous sarcoma virus; this was shown by the complement fixation test, immunofluorescent test, electron microscopy and labelling with 3H-uridine peak in sucrose gradient. Biological properties of the new synthesized virus differed from that of the parent Schmidt-Ruppin strain; the range of the sensitive cells and the protein envelope antigens altered in particular. Analogous results by the change of the viral biological properties produced in the unnatural host tissue were obtained for polioma virus synthesized in the human embryo fibroblasts transformed by this virus.

Antigens, Viral↗