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Biomedical subjects

V K Podgorodnichenko

Publications and source records attributed to V K Podgorodnichenko.

At least 19 recordsLinked to original sources
PubMed2001

[Functional suitability of iodinated casein in the prevention of iodine deficiency].

At least 1.5 billion in the world and over 100 million Russians live in iodine-deficiency areas. The sequelae of this condition are well known. The paper discusses the properties of the iodinated lactoprotein caseoiodine designed by the MRRC researchers and its capacities and advantages for using in the prevention of iodine deficiency. The assimilation of iodine from caseoiodine corresponds to the body's status: it significantly accumulates more frequently in the thyroid in dietary iodine deficiency (Group 1) and to a lesser degree in adequate dietary iodine intake (Group 2) and, by hour 72, amounts to 36.3 and 31.4% of the ingested amount, respectively (P < 0.05). The findings are in agreement with the data available in the literature on the involvement of hepatic enzymes in regulating the metabolism of iodine during its intake in the protein (casein)-bound form and in increasing its fecal excretion (up to 40% in Group 2) whereas 95% iodine excrete with urine when inorganic iodine is consumed.

Animals↗
PubMed1996

Prognostic significance of S-phase fraction detected by antithymidine antibodies in epidermoid cervix carcinomas.

PURPOSE: To assess the predictive value of pretreatment proliferative activity of epidermoid cervix carcinoma cells with respect to short- and long-term results of radiotherapy. METHODS AND MATERIALS: The proliferative activity of 25 epidermoid cervix carcinomas was evaluated as the immunofluorescent labeling index (LI) by rabbit antithymidine antibodies reacting specifically with single-stranded DNA of replication forks in S-phase cells. The short-term clinical outcome was estimated at 3-6 months after treatment by visual and palpatory examination. Three-year follow-up data were obtained through hospital charts and correspondence with referring physicians for only 19 patients. RESULTS: There was no statistically significant association between LI and such conventional prognostic factors as clinical stage. The LI value of cervix carcinomas was significantly associated with complete regression at 3-6 months after radiotherapy and 3-year disease-free survival. Complete regression at 3-6 months was observed in 87.5% patients with fast proliferating tumors (LI > 7.0%), and only in 41.2% patients with slowly proliferating tumors (p = 0.03). Probability of 3-year disease-free survival was 85.7% in patients with fast proliferating tumors and 50.0% in those with slowly proliferating tumors (p = 0.05). CONCLUSIONS: The immunofluorescent LI of epidermoid cervix carcinoma is able to provide prognostic information on short-term tumor response to radiotherapy and disease-free survival.

Adult↗
PubMed1991

A monoclonal antibody to DNA modified with osmium tetroxide/2,2'-bipyridine.

A murine monoclonal antibody (IgG) has been generated that binds to DNA modified with osmium tetroxide in the presence of 2,2'-bipyridine and does not interact with unmodified DNA. Reactivity of the antibody was tested by gel retardation assay, ELISA, dot-binding assay and immunoblotting. The results obtained suggest that the antibody does not cross-react with modified or unmodified RNA or proteins. The high specificity of the binding reaction is due to the specific recognition of modified deoxythymidine residue by the monoclonal antibody. A possible way of using the antibody produced is discussed.

2,2'-Dipyridyl↗
PubMed1990

[Clinico-diagnostic significance of fibronectin determination in diabetes mellitus in children].

Immunoelectrophoresis was used to measure fibronectin content in blood plasma of 111 children with insulin-dependent diabetes mellitus and in 20 normal children. In patients with diabetes mellitus, the content of plasma fibronectin turned out to be higher depending on the disease standing, the presence of microangiopathies, and status of compensation. It has been noted that fibronectin was detectable in urine in diabetic angionephropathy.

Adolescent↗
PubMed1986

[Determination of the DNA-synthesizing cell count by the immunofluorescence method].

In DNA-synthesizing cells DNA is partially single-stranded. Anti-thymidine antibodies, while specifically reacting with this DNA, form a complex which may be revealed using indirect immunofluorescent technique. A comparative determination of DNA-synthesizing cell number in tumor tissue (larynx squamous cell carcinoma) was performed using immunofluorescent technique and radioautography. The former method showed the labeling index (LI) to vary from 1.2 to 9.9%, while the latter showed it to vary from 1.0 to 8.2%. The correlation ratio between the LI values obtained by the two techniques was 0.79. To eliminate a possible reaction of anti-thymidine antibodies with cellular RNA, specimens were preincubated in solutions with RNAase. No more than 6 hours were required to stain specimens using this LI estimation technique. This investigation allows to reveal DNA synthesizing cells not only in the periphery of a histological section, as does routinely radioautography, but also in its centre.

Animals↗
PubMed1985

[Spontaneous secretion of antinuclear factors in systemic lupus erythematosus, rheumatoid arthritis and rheumatism].

The authors studied the ability of non-stimulated short-term cultures of peripheral lymphocytes of SLE, RA and rheumatic fever patients to spontaneously produce antibodies to DNA and other antinuclear factors. To demonstrate antibodies to DNA, use was made of the radioimmunoassay and the passive hemagglutination test. ANF-test of indirect immunofluorescence. The nosological and clinical features of secretory ANF were explored in the diseases in question. It was established that patients with the main rheumatic diseases are different as regards the rate of demonstration, level and spectrum of secretory ANF. They also differ from the group of donors in terms of the same characteristics. The culture of peripheral lymphocytes of SLE patients has the greatest ability to secrete antibodies to DNA and ANF of the primarily marginal and homogenous types of fluorescence. This characteristic correlates with SLE activity before the disease onset and over time as well as with lupus nephritis. In RA and rheumatic fever, this characteristics correlates with the disease activity, the presence of visceritis in RA. The clinicopathogenetic importance of secretory ANF in rheumatic diseases is discussed.

Antibodies, Antinuclear↗
PubMed1984

[Transport of 125I-poly I : poly C incorporated in liposomes from the enteric cavity to the small intestine mucosa. An electron microscopic autoradiographic study].

Transport of 125I-poly(I) : poly(C) incorporated into liposomes trough the small intestine mucose was investigated by electron microscopic autoradiography. With the migration of liposomes into the mucous layer on the luminal surface of the intestine up to the glycocalix level of microvilli these undergo degradation with the formation of monolayer liposomes from which polynucleotide is released. Later on the poly(I) : poly(C) or its fragments transported through the enterocytes to be accumulated in cells of the connective tissue stroma of the small intestine mucose. Part of polynucleotide was incorporated up to the arterial and lymphoid capillary level. Apparently, on the way of its transport the polynucleotide is affected by pancreatic and tissue nucleases. The accumulation of polynucleotide in macrophages, fibroblasts, lymphocytes, plasma cells and smooth muscle cells was traced. It is supposed that the polynucleotide accumulated in stroma of the small intestine mucose may preserve its interferon inducing activity.

Animals↗
PubMed1982

[Induction of interferon production with dextran sulfate].

Interferon-inducing activity of polyanions (dextran Sulfate, native DNA, polyphosphate, polypentose, polyanetole sulfate, heparin) was studied. Dextran sulfate applied parenterally and enterally was found to be capable of inducing endogenous interferon production in white mice. The maximal titers of interferon (160 units/ml) were seen in the blood serum of mice upon administering the preparation in a dose of 20 mg/kg body mass. No substantial differences were discovered in the time course of interferon production whatever the route of administration.

Animals↗
PubMed1980

Antibodies to natural double-stranded RNA and to synthetic polyribonucleotides in the sera of patients with systemic lupus erythematosus.

A parallel testing of antibodies to double-stranded ribonucleic acid in 80 sera from patients with systemic lupus erythematosus by the membrane binding method using natural 3H-dsRNA preparation and synthetic 125I-poly I. poly C preparation revealed a good correlation (r = +0.81). In a selected set of patients with SLE we observed a slight tendency to preferential binding of the natural preparation and a lower frequency of anti-dsRNA antibodies when compared to that reported by other investigators. The presence of anti-dsRNA did not correlate with the presence of anti-dsDNA.

Antibodies↗
PubMed1979

Clinical importance of antibodies against double-stranded ribonucleic acid in systemic lupus erythematosus and similar diseases of connective tissue.

Prolonged repeated analyses of sera from 98 patients with systemic lupus erythematosus (SLE) and 76 patients with similar diffuse diseases of connective tissue by a radioisotope membrane binding method for the assessment of antibodies against double-stranded ribonucleic acid (anti-ds-RNA) revealed that these antibodies are most frequently encountered in patients with the evolutive form of SLE (53%), but also in approximately one fifth of patients with non- evolutive SLE and in patients with similar diffuse diseases of connective tissue. Anti-ds-RNA are not linked with stages of clinical activity; their occurrence is on the whole independent on the occurence of antibodies against ds-DNA and nuclear ribonucleoprotein. There is a very close association with the incidence of antibodies against denatured DNA and a substantially small association with antibodies against acid nuclear antigen (Sm). The occurrence of anti-ds-RNA is a favourable prognostic sign, as in the majority of patients who had such antibodies at any time in their serum, later the disease can be suppressed by treatment, while patients who never had anti-ds-RNA in serum remain for a long time in the active stage of the disease.

Autoantibodies↗
PubMed1978

[Induction of antibodies to native DNA by components of Bordetella pertussis].

Administration of Bordetella pertussis and some of their components to mice induced an increase of DNA-binding activity of the sera revealed under ionic strength conditions of physiological saline, mostly on the 14th day. It was shown by the inhibition method that interaction between mouse sera and native DNA was specific. Maximum increase in the quantity of mouse sera proteins reacting with DNA under low ionic strength condition of physiological saline (0.05 M NaCl) is revealed on the 7th day. However, in administration of Bordetella pertussis and their cytoplasmic membrane the elevated DNA-binding proteins content persisted up to 14 days.

Animals↗