Fractions of acid and base blood serum proteins, separated by ion exchange chromatography on QAE-Sephadex (but not the proteins of the whole blood serum, which had the same charge), reacted with DNA preparations. Separate fractions of blood serum proteins were able to react with DNA after electrophoretic separation. Binding of blood serum proteins with DNA did not depend on ion strength within the range of NaCl concentration from 0.1 M to 0.5 M; it was also stable at pH 5.5 = 8.0. Interaction between acid DNA-binding proteins and native DNA was inhibited by denatured DNA, polyguanilic and polyinosinic acids and by other polyanions: dextran sulfate, polyvinyl sulfate, polyanetol sulphonate, polystyrol sulphonate and heparin. Acid DNA-binding proteins showed only a slight affinity to polyadenilic, polyuridilic and polycytidilic acids. The acid and base DNA-binding proteins appear to be contained in blood serum in the form of a loosely bound complex.

Blood Proteins↗

PubMedDate not supplied

[Antibodies to double-helical RNA and several viruses in medical personnel caring for systemic lupus erythematosus patients].

An average level of antibody to double-stranded RNA in patients with systemic lupus erythematosus and medical staff taking care of these patients is higher than that in physicians from other institutions and donors. The level of antibodies to antigens of various viruses in lupus patients is elevated in comparison with that in the medical staff. No statistically significant differences in the levels of antibody to viruses in the staff of various institutions were found.

Adult↗

PubMedDate not supplied

[A study of the reaction between antibodies and DNA by the method of immune complex formation on the membranes of nitrocellulose filters].

The conditions were developed to measure antibodies to denatured and native DNA. It was shown that membrane filters treated with alkali adsorbed denatured DNA complexed with antibodies. Utilizing this method it was shown that antibodies to denatured DNA react with pyrimidines (probably thymine). This assay provides proof of the wide existence antibodies to native DNA in the sera of patients with systemic lupus erythematosus. DNA-binding of sera was significantly depended on reaction condition.

Animals↗

PubMedDate not supplied

[Study of nucleic acid structure by immunochemical methods. I. Antibodies specific for 6-sulfo-5,6-dihydro-4-methoxyaminopyrimidinone-2].

Immunization of animals with DNA modified by a mixture of bisulphite and O-methylhydroxylamine and methylated bovine serum albumin results in production of antibodies mainly reacting with modified DNA. Antibodies that react with denatured DNA were produced in minute quantity. It was shown that elicited antibodies possess a high specificity and have the ability to recognize only nucleotides with a double modification. The immune sera were fractionated by Sephadex G-200 column chromatography and the antibody activity was demonstrable in the 19S and 7S fractions. The attempts to induce synthesis of antibodies by injection of DNA modified by O-methylhydroxylamine failed.

Animals↗

PubMedDate not supplied

[Antibodies to the thyroid microsomal antigen in children and adolescents subjected to radiation exposure as a result of the accident at the Chernobyl Atomic Electric Power Station].

For evaluation of the possibility of the appearance of autoimmune thyroiditis in children and juveniles lived in the areas of Kaluga Province [correction of region] suffered from the Chernobyl accident the content of antibodies to human thyroid microsomal antigen was investigated. Percentage of positive sera varied from 4.8% to 1.2% during 6 years. There is significant difference in the frequency of the antibody appearance between persons suffered from radioactive iodine and unsuffered ones. Correlation between levels of antimicrosomal antibodies and radiation dose obtained from incorporated radioactive iodine was not estimated.

Accidents, Occupational↗

PubMedDate not supplied

[Anion exchange chromatography of proteins leads to induction of DNA-binding activity in them].

The present work is devoted to investigation of the acquired DNA-binding activity of several proteins, such as bovine serum albumin, myoglobin, and collagens in different conformational states (native, denatured, and gelatin) following their anion exchange chromatography. Induction of DNA-binding activity, as shown, is natural phenomenon common in protein treatment on positively charged matrix. Obviously, anion exchange chromatography of proteins results in their structural conformational changes. It was suggested that the DNA-binding activity of activated proteins is based on the ability of the protein molecule to accept additional protons upon contact with the anion exchanger. This hypothesis is confirmed by the inhibition of the interaction of activated protein with DNA in the presence of Na-EDTA.

Anion Exchange Resins↗

PubMedDate not supplied

[Immunologic methods in epidemiologic monitoring of population, affected by radioactive iodine as a result of the accident at the Chernobyl nuclear plant].

Ultrasound investigations of the thyroid gland and determinations of microsomal antibodies have been performed in persons who lived in the town of Korosten (Zhitomir Region) during the Chernobyl accident. A high correlation has been found between ultrasound and immunological results. The immunological screening of the population suffered from the Chernobyl disaster might be successfully used for the autoimmune thyroiditis detection. These data complete those obtained by the ultrasound tests.

Accidents, Occupational↗

PubMedDate not supplied

[The probable sequelae of thyroid damage from radioactive iodine during the Chernobyl accident].

An important peculiarity of the Chernobyl catastrophe is the discharge into the atmosphere of tremendous amount of radioactive iodine and, as a result, selective damage of the thyroid in children from the affected areas. The most dangerous consequence is the thyroid cancer. The analysis of the situation when children's thyroids were subjected to irradiation shows that tumors can most frequently develop as late as 20-30 years after irradiation. There are reasons to believe that tumors are induced by low dose of irradiation. The most important factor in development of pathologies is for sure the age of the children of the moment of irradiation. A well-known consequence of the impact of radiation on the thyroid is the lymphocyte thyroiditis. The interest to this pathology is determined by the fact that it substantially increases the probability of development of various haematologic diseases (lympho- and myeloproliferative neoplasms).

Adolescent↗