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Biomedical subjects

V K Pruthi

Publications and source records attributed to V K Pruthi.

17 recordsLinked to original sources
PubMed2001

Immunohistochemical study of vestibular gingival blood vessel density and internal circumference in smokers and non-smokers.

BACKGROUND: This study was undertaken to determine: 1) if sections stained with monoclonal antibody to CD34 can be used for measurement of gingival blood vessel density and circumference and 2) if long-term smoking affects either of these 2 parameters. METHODS: Vestibular gingival biopsy samples were obtained from 17 adults (5 smokers and 12 non-smokers) who were undergoing routine periodontal surgery independently of this study. Hematoxylin and eosin staining was used for histological assessment of inflammation, while immunohistochemical staining with CD34 mouse monoclonal antibody was used to mark the endothelial cells of the blood vessels within each sample. Stringent selection criteria were applied to the 17 biopsy samples, and 7 (from 3 smokers and 4 non-smokers) were chosen to have their vascular density (number of vessels/unit area) and internal vessel circumference (IC) measured under a blinded protocol. RESULTS: Repeated measurements of vascular density and internal circumference showed small standard deviations, indicating the high reproducibility of the measurement technique. The smoker group showed a higher proportion of small (IC < 50 microns) and a lower proportion of large blood vessels (IC > 100 microns), which was statistically significant (P = 0.0403 and P < 0.001, respectively). The difference between the vascular density of smokers and non-smokers was not significant (P = 0.092). CONCLUSIONS: The present study indicates that the CD34 immunohistochemical staining technique is a simple and reproducible method for the measurement of density and internal circumference of gingival blood vessels. Based on this technique, cigarette smokers show a higher percentage of smaller blood vessels and a lower percentage of larger vessels, but similar vascular density, to non-smokers.

Adult↗
PubMed1995

Prevalence of nifedipine-induced gingival hyperplasia.

This study investigated the prevalence of gingival hyperplasia in subjects who were undergoing treatment with a calcium channel blocker, nifedipine, in dentate and edentulous subjects. In addition, the relationship between gingival hyperplasia and 5 other variables (duration of nifedipine intake, dose, age, oral hygiene index, and number of teeth) was investigated. Results of statistical analyses showed overwhelming evidence that the percent of nifedipine subjects with gingival hyperplasia was greater than that of the control subjects (P < 0.05) and that, across treatment group, the percent of dentate subjects with hyperplasia was significantly higher than that for edentulous subjects (P < 0.05). The chi2 test showed a strong univariate relationship between gingival hyperplasia and, separately, oral hygiene and number of teeth. For 24% of the subjects with gingival hyperplasia index (GHI) values of 2 or 3, gingivectomy was performed, and the tissues were prepared for histological examination. Microscopic examination of the biopsy material showed findings similar to those from previously reported cases of nifedipine- or phenytoin-induced hyperplasia.

Age Factors↗
PubMed1993

The effectiveness of the Nd:YAG laser in the treatment of dental hypersensitivity.

Dentin hypersensitivity is one of the most painful and least predictably treated chronic conditions in dentistry. The effectiveness of laser therapy in the reduction of dentin hypersensitivity and its effects on pulpal vitality were studied. Nineteen subjects participated in the randomized, double-blind study, and were followed up for three months. Two sites were treated. One received helium neon (He:Ne) laser treatment and the other received He:Ne plus Nd:YAG (He:Ne+Nd:YAG) laser treatment. Laser treatment consisted of 30 millijoules (mJ) to 100 mJ per pulse, at 10 pulses per second (pps) in increments of 10-40 seconds each over a total treatment time of less than two minutes, without local anesthesia. Hypersensitivity was assessed by mechanical stimulus (using a sharp explorer), and thermal stimulus (using a blast of cold air from a dental syringe). Pulpal vitality was measured using an electrical stimulus. The results indicate that immediately following laser treatment and for three months thereafter, the subjects' perceived level of discomfort decreased. He:Ne treatment reduced dentin hypersensitivity to air by 63 per cent and to mechanical stimulation by 61 per cent over three months. The He:Ne + Nd:YAG treatment reduced dentin sensitivity to air by 58 per cent and to mechanical stimulation by 61 per cent. All teeth remained vital after laser treatment, with no adverse reactions or complications. He:Ne and He:Ne + Nd:YAG laser treatment can be used to reduce dentin hypersensitivity without detrimental pulpal effects.

Adult↗
PubMed1990

Localized juvenile periodontitis: a case analysis and rational approach to treatment.

This case involves a 14-year-old female patient affected with localized juvenile periodontitis. Treatment consisted of periodontal flap surgery in conjunction with tetracycline therapy. A successful result was obtained and maintained. The case depicts the fact that in many instances localized juvenile periodontitis can be treated with confidence and a high degree of predictability. It should also be noted that, like all treatment modalities, the treatment of this disease as discussed in this case is not the panacea and will undergo considerable changes in the future; however, in light of current knowledge, this combined treatment approach appears to be effective in LJP patients.

Adolescent↗
PubMedDate not supplied

Serological investigation of refractory human adult periodontitis.

This study examined the difference between the serum antibody profiles in refractory adult periodontitis patients (group A), and compared to those (group B) who responded well to conventional periodontal treatment. The levels of specific IgG antibody to Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, Fusibacteriumnucleatum, and Eikenella corrodens were assessed in a group of 19 patients (group A) and 11 patients (group B). Specific IgG serum antibody levels were estimated using biotin-avidin linked immunosorbent assay (BALISA). Results indicated that Actinobacillus actinomycetemcomitans, and Bacteroides gingivalis had very high levels of specific circulating antibody in the sera of both groups of patients; whereas, Fusobacterium nucleatum and Eikenella corrodens showed considerable lower levels of antibody than the other two antigens. However, the differences between the two groups with regard to the antibody levels against different bacterial antigens were not statistically significant.

Actinobacillus↗