PubMed Health⌕ Search

Biomedical subjects

V K Vasil'ev

Publications and source records attributed to V K Vasil'ev.

At least 19 recordsLinked to original sources
PubMed1987

[Effect of the level of free-radical lipid peroxidation on the ribonucleotide reductase activity of liver tissue].

The dynamics of RNA and DNA synthesis as well as the activity of free radical processes in rat liver during the first 40 hours after partial hepatectomy was studied. It was shown that RNA synthesis activation follows the activation of lipid peroxidation (LPO), whereas the DNA synthesis activation follows the decrease of the LPO level. These facts are suggestive of the dependence of the ribonucleotide reductase (RNR) activity on free radical processes. This observation was confirmed by a RNR activity analysis of regenerating liver homogenates. The activity peak was shown to precede the peak of DNA synthesis. Evidence for free radical RNR suppression was also obtained in direct experiments, using intact animal liver homogenates, to which a natural antioxidant (tocopherol) was added.

Animals↗
PubMed1984

[Age-related characteristics of poststress DNA repair in the myocardium].

It has been demonstrated that young and adult rats had age-associated differences in stress-induced lesions and in poststressor repair of DNA synthesis in the myocardium. In both age groups, the DNA repair systems perform an identical scope of work aimed at abolition of poststressor lesions with the difference that in young rats, the repair is completed 2 days and in adult rats 3 days after exposure to stress is discontinued.

Aging↗
PubMed1983

[Changes in the primary structure of DNA in rat organs during the aging process].

Thin-layer chromatography was used to study the composition of bases and pyrimidine sequences of varying lengths (isopliths) in DNA of different organs of rats aged 1, 12 and 30 months. It was found that with age the isoplith composition of DNA in the organs under study changed towards the chaotic distribution. The composition of DNA bases changed with age towards enrichment with guanine-cytosine-vapors, which was particularly pronounced in organs marked by an age-related decrease of 5-methylcytosine in DNA.

Aging↗
PubMed1982

[DNA changes in a nonischemic area of the rat myocardium in experimental myocardial infarct and its prevention].

DNA damage and repair in the nonischemic area of the heart were analyzed after experimental myocardial infarction to evaluate the effect on these processes of the beta-blocker inderal and lipid peroxidation inhibitor ionol. It was found that in the nonischemic area of the heart, DNA damage was manifested by the decreased polymerism averted to a significant degree by inderal and ionol which interfered with postinfarction activation of lipid peroxidation. Accordingly, inderal and, to a greater degree, ionol reduced postinfarction activation of DNA repair synthesis in the nonischemic area of the heart.

Adrenergic beta-Antagonists↗
PubMed1981

[Myocardial DNA damage and repair in emotional-painful stress].

[Partial depolymerization of myocardial DNA was found to ensue within the first hours after termination of stress exposure. The depolymerization was accompanied by DNA synthesis repair manifesting both in in-vivo and in-vitro experiments. With time the repair of DNA synthesis decreased while its polymerism got recovered. The data obtained provide evidence in favour of the relationship between the stress-induced free radical activation and damage and subsequent DNA synthesis repair.

Animals↗
PubMed1981

[Cephalexin pharmacokinetics].

The pharmacokinetics of cephalexin monohydrate after its oral administration in a single dose was studied on rats and dogs. The analysis of the pharmacokinetic data obtained with the one-compartmental model showed that the rate of the antibiotic absorption in the rats was higher than that in the dogs. The periods of the cephalexin half-absorption and maximum concentration were 0.2 and 1.1 hour in the rats and 0.64 and 1.9 hours in the dogs respectively. The period of the antibiotic half-life was almost the same in both animal species. Selective localization of cephalexin in the kidney and liver tissues was noted. The antibiotic was mainly excreted by the kidneys (98.8 per cent for 24 hours).

Animals↗
PubMed1979

[Bioavailability of ampicillin suspensions].

The rate of ampicillin transfer into solution from suspensions of 2 types, i.e. suspensions of anhydrous ampicillin and suspensions of ampicillin trihydrate was studied. Conditions for estimation of the rate of ampicillin dissolution from the suspensions were developed. The pharmacokinetics of the pharmaceutical forms was studied on dogs. Definite advantages of the anhydrous ampicillin suspension were shown as compared to the ampicillin trihydrate suspension.

Ampicillin↗
PubMed1978

[Experimental study of a gentamycin ointment].

Technology of 0.1 per cent gentamicin ointment production was developed. The ointment base consisted of vaseline and parafin (95:5). Pharmacokinetics and innocuousness of the gentamicin ointment were studied. It was shown that the ointment provided gentamicin diffusion through the skin utegument during a long period of time. Histological studies showed no local irritating effect of the ointment on the skin in its local use.

Administration, Topical↗
PubMed1977

[Study of the pharmacokinetics of dicloxacillin used parenterally in an experiment].

Dicloxacillin was superior to oxacillin in the maximum levels and retention time in the blood of dogs and rats. Dicloxacillin was intensively excreted by the kidneys for 3 hours. For 24 hours 62 per cent of the drug was excreted with the urine. In intravenous administration to animals the peaks of the antibiotics levels in the blood and internal organs were achieved at earlier periods, while the circulation time was shorter than in intramuscular administration. The intramuscular use of the drugs had no advantages in the kinetics against the oral use.

Animals↗
PubMed1976

[Pharmacokinetics of penicillins in the blood of dogs administered the combination preparation, ampiox, internally].

The pharmacokinetics of penicillins in the blood of dogs treated with ampiox, a combination of ampicillin and oxacillin at a ratio of 1 : 1 was studied. The drug was administered orally in single or repeated doses of 25, 50 and 100 mg/kg. The maximum levels of ampicillin in the blood serum were observed 1 hour after a single administration of the drug. The therapeutic concentrations of the antibiotic were preserved for 6 hours, its value being depended on the dose used. The maximum concentration of oxacillin was detected 1 hour after the drug administration in various doses and it was preserved in the blood at the therapeutic levels for 3 hours. The dynamics of circulation of ampicillin and oxacillin administered separately did not differ from that established for the use of ampiox. The regularities of the pharmacokinetics of ampiox on its repeated use remained practically unchanged.

Administration, Oral↗
PubMed1976

[Results of an experimental study of the pharmacokinetics of polymyxin B sulfate].

Pharmacokinetics of polymyxin B sulfate of Soviet production was studied in various species of animals with the use of different administration routes and dosage. After a single intramuscular administration of the drug to dogs in doses of 1.1 and 2.2 mg/kg the antibiotic was detected within 5 hours at the maximum level during the 1st hour. A two-fold increase of the dose was accompanied by 1.5 times increase in the antibiotic level. Repeated administrations of polymyxin B sulfate in a dose of 4.5 mg/kg did not result in an increase in the blood level as compared to a single use of the drug. When polymyxin B sulfate was administered intravenously, the concentration peak was observed in 15 minutes independent of the dosage. Later the antibiotic level decreased. The maximum level of the drug in the mice was observed 1 hour after its intramuscular administration in a dose of 8 mg/kg, the highest levels being registered in the kidney tissues and urine.

Animals↗
PubMed1976

[Pharmacokinetic characteristics and the chemotherapeutic effectiveness of a 2% ditetracycline-erythromycin eye ointment].

The study of the antibacterial activity of ditetracycline combination with erythromycin (ratio 1 : 1) showed that the effect of the antibiotics increased with respect to most of Staphylococcus and Coli bacteria. The chemotherapeutic effect of the ointment in treatment of experimental staphylococcal conjunctivitis of rabbits was also studied. The clinical signs of the disease disappeared earlier than in the experiments with the use of ditetracycline and erythromycin ointments alone. The experiments with the rabbits revealed a prolonged effect of the ointment: ditetracycline and erythromycin in therapeutic concentrations were detected in the conjunctiva and cornea after a single application for 24--48 and 8 hours respectively.

Animals↗