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Biomedical subjects

V Krumphanzl

Publications and source records attributed to V Krumphanzl.

17 recordsLinked to original sources
PubMed1987

New trends in microbial technology.

Microbial technology includes not only the production of materials in bioreactors, or the production of new catalysts by genetic engineering but extends to aspects of both human and animal health care, waste and pollution management, enhanced oil recovery, mineral leaching, advanced plant breeding, diagnostics and analytical equipment, biosensors, bioelectronics and renewable energy system based on biomass feedstocks. National strategies of industrialized countries are being developed which identify microbial technology as a substantial factor in the attainment of industrial and economic goals. Although extremely promising microbial technology is not a quick fix and its application will only arise as a result of systematic programme of research and development. Such programme requires a broad base of disciplinary underpinning in molecular biology, genetics and bioengineering. The development of expertise of this kind in the tertiary educational institutions is the essential starting point. It should be developed by appropriate programmes and networking systems.

Animals↗
PubMed1986

Biosynthesis of monensins A and B: the role of isoleucine.

Isoleucine added to the cultivation medium of Streptomyces cinnamonensis C-100-5 induced a relative increase of the production of monensin B at the expense of monensin A. U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of 1-13C-2-methylbutyrate into monensins A and B showed the label to be evenly incorporated in both products at carbon atoms originating from C(1) of propionate. In regulatory mutants insensitive to 2-amino-3-chlorobutyrate isoleucine influenced the production of monensins only slightly but strains resistant to 2-aminobutyrate and norleucine decreased their total production by 2-12% in the presence of isoleucine which was associated with a decrease of monensin A content by 14-52%. The inhibitory effect of isoleucine on the biosynthesis of valine, a specific precursor of the butyrate unit of monensin A, is discussed.

Aminobutyrates↗
PubMed1985

New bacteriophage-like particles in Corynebacterium glutamicum.

Three new phage-like particles (CG1, CG2, and CGK1) were isolated from Corynebacterium glutamicum CBII. Particles CG1 and CG2 are DNA phages with long, noncontractile tails, CGK1 is a killer particle according to electron microscopy. A heat-stable low-molecular-weight bacteriocidal substance affecting various coryneform bacteria was observed to be joined to the killer particle CGK1.

Bacteriocins↗
PubMed1985

Effect of precursors on biosynthesis of monensins A and B.

Precursors of monensins (acetate, propionate, butyrate, isobutyrate) affect the total production and the relative proportion of monensins A and B. Addition of propionate into the fermentation medium causes a prevalence of monensin B whereas butyrate and isobutyrate stimulate the production of monensin A and suppress the production of monensin B.

Acetates↗
PubMed1984

Improving the production of monensin by Streptomyces cinnamonensis.

The production of monensin by Streptomyces cinnamonensis was increased by genetic improvement of the strain and by modification of cultivation conditions. The selection of a suitable strain and optimization of the fermentation process (temperature, aeration, addition of esters of oleic acid) resulted in a 30 fold increase of the monensin production.

Anti-Bacterial Agents↗
PubMed1982

Anaerobic degradation of cellulose and formation of methane.

The existing knowledge of anaerobic digestion of cellulose-containing wastes and methane formation is reviewed. Mutual relationships between the individual phases of this complex process and the mechanism of methane biosynthesis are discussed in three sections: (1) Non-methanogenic phase and digestion of cellulose; (2) methanogenic phase and methanogenesis; (3) mixed cultures and their advantages.

Anaerobiosis↗
PubMed1980

The extracellular system of beta-1,3-glucanases of Alternaria tenuissima and Aspergillus vesicolor.

During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affinity for yeast glucan were isolated by affinity chromatography and further characterized.

Alternaria↗